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Murine 3t3 l1 cells

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Murine 3T3-L1 cells are a well-established cell line derived from mouse fibroblasts. These cells are commonly used as a model for adipocyte (fat cell) differentiation and metabolism studies.

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Lab products found in correlation

Exo red, by System Biosciences (2 mentions) Pkh67, by Merck Group (2 mentions) Sp5 tandem scanner spectral 2 photon confocal microscope, by Leica (2 mentions) Differentiated human adipocytes, by Lonza (2 mentions) Exo green, by System Biosciences (2 mentions) Isobutylmethylxanthine, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Troglitazone, by Merck Group (1 mentions) 3t3 l1 cells, by Merck Group (1 mentions)

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3T3-L1 cells (146 citations) 3T3 cells (18 citations) Murine preadipocyte (3T3-L1) cells (2 citations)

4 protocols using murine 3t3 l1 cells

1

Exosome Labeling and Uptake Assay

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Purified exosomes from either human or mouse plasma samples were labeled with green fluorescent linker PKH67 (Sigma-Aldrich, St. Louis, MO), Exo-Red, and Exo-Green (System Biosciences, Mountain View, CA), and further incubated at 37°C for 10 min, until the reaction was stopped by adding ExoQuick-TC reagent. Labeled exosomes were added to either differentiated human adipocytes (# PT-5006, Lonza, Walkersville, MD) or murine 3T3-L1 cells (ATCC, Manassas, VA) for 4 hours in a cell culture incubator at 37°C. Labeled exosomes were monitored for delivery into target cells using a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) with a 63× oil-immersion lens.
Khalyfa A., Gozal D., Masa J.F., Marin J.M., Qiao Z., Corral J., González M., Marti S., Kheirandish-Gozal L., Egea C., Sánchez-Quiroga M.Á., de Terreros F.J, & Barca F.J. (2018). Sleep-Disordered Breathing, Circulating Exosomes, and Insulin Sensitivity in Adipocytes. International journal of obesity (2005), 42(6), 1127-1139.
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2

Exosome Labeling and Uptake Assay

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Purified exosomes from either human or mouse plasma samples were labeled with green fluorescent linker PKH67 (Sigma-Aldrich, St. Louis, MO), Exo-Red, and Exo-Green (System Biosciences, Mountain View, CA), and further incubated at 37°C for 10 min, until the reaction was stopped by adding ExoQuick-TC reagent. Labeled exosomes were added to either differentiated human adipocytes (# PT-5006, Lonza, Walkersville, MD) or murine 3T3-L1 cells (ATCC, Manassas, VA) for 4 hours in a cell culture incubator at 37°C. Labeled exosomes were monitored for delivery into target cells using a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) with a 63× oil-immersion lens.
Khalyfa A., Gozal D., Masa J.F., Marin J.M., Qiao Z., Corral J., González M., Marti S., Kheirandish-Gozal L., Egea C., Sánchez-Quiroga M.Á., de Terreros F.J, & Barca F.J. (2018). Sleep-Disordered Breathing, Circulating Exosomes, and Insulin Sensitivity in Adipocytes. International journal of obesity (2005), 42(6), 1127-1139.
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3

3T3-L1 Adipocyte Differentiation

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Murine 3T3-L1 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured in DMEM with 10% fetal calf serum. Confluent 3T3-L1 cells were treated with a cocktail of 0.5 mM isobutylmethylxanthine, 1 mM dexamethasone, 5 mg/ml insulin, and 5 mM troglitazone (all from Sigma–Aldrich, St Louis, MO, USA) to induce differentiation. After 2 days of differentiation, the cells were maintained in a medium with insulin until harvest. Experiments were conducted using differentiated adipocytes (10–14 days).
Hu W., Xiong H., Ru Z., Zhao Y., Zhou Y., Xie K., Xiao W., Xiong Z., Wang C., Yuan C., Shi J., Du Q., Zhang X, & Yang H. (2021). Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer cachexia. Cell Death & Disease, 12(1), 134.
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4

Murine 3T3-L1 Cell Differentiation

Cited 1 time
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Murine 3T3-L1 cells (American Type Culture Collection) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum and 100 μg/mL penicillin/streptomycin in a humidified atmosphere of 5% CO2 at 37°C. Cells were differentiated and analyzed as previously described [10 (link)]. Immunostaining was performed on differentiated day 8.
Shong K.E., Oh C.M., Namkung J., Park S, & Kim H. (2020). Serotonin Regulates De Novo Lipogenesis in Adipose Tissues through Serotonin Receptor 2A. Endocrinology and Metabolism, 35(2), 470-479.
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