TS, VS, total ammonia nitrogen (TAN), and total alkalinity were analyzed according to Standard Methods.31 VFAs (acetate, propionate, and butyrate) were measured with high-performance liquid chromatography (HPLC, Agilent 1260, USA) as previously described.30 (link) Elemental composition (carbon and nitrogen) of freeze-dried CM and CS were measured using an elemental analyzer (Vario EL cube, Germany). A pH-meter was used to measure pH (HACH, USA). Electrical conductivity was measured with a conductivity meter (Mettler Toledo, USA). Free ammonia nitrogen (FAN) was calculated from TAN concentrations according to a previously described formula.32 (link) Gas volume from the gas collection bag was measured with a digital mass flow meter (Omega, USA) every 24 hours. Biogas composition (CO2, CH4) was determined by a 7890B gas chromatograph system (Agilent, USA) equipped with a thermal conductivity detector.
7890b gas chromatograph system
Manufactured by Agilent Technologies
Sourced in United States
The 7890B gas chromatograph system is a versatile analytical instrument designed for the separation and detection of chemical compounds. It employs gas chromatography to provide efficient and reliable separation of complex mixtures, enabling the identification and quantification of individual components. The system is capable of handling a wide range of sample types and can be configured with various detectors to suit the specific needs of the application.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using 7890b gas chromatograph system
1
Comprehensive Biogas Production Analysis
2
SCFA Analysis by GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For SCFA analysis, 50% H2SO4 (50 μL) was added to serum (50 μL) for acidification, and an internal standard (2-Methylvaleric acid) was used for gas chromatography-mass spectrometry (GC-MS) analysis. An Agilent 7890B gas chromatograph system coupled with an Agilent 5977B mass spectrometer fitted with a capillary column (HP-FFAP 30 m × 250 μm × 0.25 μm) was used for GC-MS analysis. An Agilent chemstation was used for chromatographic peak extraction and quantitative analysis. The standard curve of SCFA concentration was calculated according to the ratio of SCFAs and the internal standard chromatographic peak area. Seven SCFAs, acetic acid, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid, and hexanoic acid were quantified.
3
Comprehensive GC-MS Analysis of Metabolic Profiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The analysis of all samples was performed using a 7000°C mass spectrometer coupled with a 7890 B gas chromatograph system from Agilent Technologies (CA, United States). The separation of serum, BAT, WAT, liver, spleen and kidney samples was performed using an HP-5MS fused silica capillary column. Using helium gas as a carrier, a 1 µL aliquot of the derivative solution was processed in split mode (50:1), with the front inlet purge flow rate set to 3 mL/min and the gas flow rate set to 1 mL/min. The temperatures for the administration, transfer line, and ion source were maintained at 280°C, 250°C, and 230°C, respectively. The GC temperature program started at 60°C for 4 min, followed by an increase to 300°C at a rate of 8°C/min, and then held at 300°C for 5 min. For ionization, the voltage of the electron impact was set to −70 eV, and data acquisition occ1urred at a rate of 20 spectra per second. MS identification was performed using electrospray ionization (ESI) in full scan mode, with a mass-to-charge ratio (m/z) range of 50–800.
We have effectively uploaded the source data from our GC-MS analysis to MetaboLights. The identifier MTBLS8334 has been exclusively assigned to our research project. To access our study, simply follow this link:https://www.ebi.ac.uk/metabolights/MTBLS8334 .
We have effectively uploaded the source data from our GC-MS analysis to MetaboLights. The identifier MTBLS8334 has been exclusively assigned to our research project. To access our study, simply follow this link:
4
Measuring Cecal and Fecal SCFA Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SCFA measurements were performed at TechnoSuruga Laboratory Co. Ltd. (Shizuoka, Japan). We analyzed 100 mg of the cecal content or fecal samples using a gas chromatography–flame ionization detector system (7890B Gas Chromatograph System, Agilent Technologies, Santa Clara, CA, USA) fitted with a DB-WAXetr column (30 m, 0.25-mm internal diameter, 0.25-μm film thickness; Agilent Technologies) to measure SCFA levels, as previously reported (65 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!