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Goat anti human igg hrp secondary antibody

Manufactured by Southern Biotech
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The Goat anti-human IgG-HRP secondary antibody is a laboratory tool used in immunoassays. It is a conjugate of a goat-derived antibody that binds to human immunoglobulin G (IgG) and the enzyme horseradish peroxidase (HRP). This antibody-enzyme complex can be used to detect and quantify the presence of human IgG in samples.

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Lab products found in correlation

3 3 diaminobenzidine tablets, by Merck Group (1 mentions) Sigmafast opd peroxidase substrate, by Merck Group (1 mentions)

Close spelling variants of this product

Goat anti-human IgG-HRP (33 citations) Anti-human IgG-HRP (13 citations) HRP-conjugated goat anti-human IgG secondary antibody (3 citations) Goat anti-human IgG HRP antibody (2 citations) Goat anti-Human IgG (monkey adsorbed)-HRP secondary antibody (2 citations)

2 protocols using goat anti human igg hrp secondary antibody

1

Viral Vector Antigenicity Evaluation

Cited 1 time
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To analyze both the exposure and antigenicity of V2 on viral vectors, vectors (7 × 109 VP for Ad, Ad-HVR1-20aa-V2 and Ad-HVR1-V2, and 2.1 × 1010 VP for Ad-pIX-V1V2) were mixed with 4x native loading buffer without boiling, and resolved on native PAGE gels, followed by transfer and blocking on PVDF membrane. Blotting was performed with HIV-1 205 F strain-derived hMAb 6.4 C (1:1,000), and with goat anti-human IgG-HRP secondary antibody (1:2000; Southern biotech, Birmingham, AL). The proteins were detected by using 3′3′-diaminobenzidine tablets (Sigma-Aldrich, MO) (Gu et al., 2013 (link)).
Gu L., Krendelchtchikova V., Krendelchtchikov A., Farrow A.L., Derdeyn C.A, & Matthews Q.L. (2015). Adenoviral vectors elicit humoral immunity against variable loop 2 of clade C HIV-1 gp120 via “Antigen Capsid-Incorporation” strategy. Virology, 487, 75-84.
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2

Quantifying Adenoviral Capsid Antigenicity

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The whole virus ELISAs were performed as described elsewhere (Gu et al., 2013 (link)), in order to investigate both the exposure and antigenicity of V2 on surface of capsid. Briefly, different amounts of vectors (2 × 109 VP/well for Ad-HVR1-20aa-V2 and Ad-HVR1-V2, and 6 × 109 VP/well for Ad and Ad-pIX-V1V2) were immobilized and blocked. The immobilized vectors were incubated with hMAb 6.4 C (1:1,000), followed by incubation with goat anti-human IgG-HRP secondary antibody (1:2000; Southern biotech, Birmingham, AL). ELISAs were developed with the SIGMAFAST OPD peroxidase substrate (Sigma-Aldrich, MO) and measured at OD 450 nm.
Gu L., Krendelchtchikova V., Krendelchtchikov A., Farrow A.L., Derdeyn C.A, & Matthews Q.L. (2015). Adenoviral vectors elicit humoral immunity against variable loop 2 of clade C HIV-1 gp120 via “Antigen Capsid-Incorporation” strategy. Virology, 487, 75-84.
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