Human gastric mucosa cell line (GES-1) used in this study was procured from BeNa Culture Collection (Beijing, China). Human GC cell lines (HGC-27, AGS) were bought from Cell Bank of the Chinese Academy of Sciences (Shanghai, China); both MKN-7 and MKN-45 cell lines were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China). RPMI-1640 commercially acquired from Thermo Fisher Scientific (Waltham, MA) was used to culture GES-1, HGC-27, MKN-7, and MKN-45 cells under 37°C and 5% CO2. AGS cells were cultivated in F12K medium (Gibco). In addition, 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) both served as medium supplements. SF1670 (1 μM), an inhibitor of PTEN (phosphatase and tensin homolog), was purchased from MedChemExpress (South Brunswick, NJ).
Sf1670
Manufactured by MedChemExpress
Sourced in United States
SF1670 is a laboratory instrument designed for the analysis and separation of chemical compounds. It utilizes a specialized technique called size exclusion chromatography to separate molecules based on their size and molecular weight. The core function of SF1670 is to facilitate the purification and isolation of target compounds from complex mixtures, enabling researchers to obtain high-purity samples for further analysis and experimentation.
Automatically generated - may contain errors
Lab products found in correlation
Ges 1, by BNCC (1 mentions)
Mkn45, by Thermo Fisher Scientific (1 mentions)
Hgc 27, by Thermo Fisher Scientific (1 mentions)
Ges 1, by Thermo Fisher Scientific (1 mentions)
F 12k medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Mkn 45, by Procell (1 mentions)
Hgc 27, by National Collection of Authenticated Cell Cultures (1 mentions)
Mkn 7, by Procell (1 mentions)
Lxr 623, by MedChemExpress (1 mentions)
Chloroquine, by MedChemExpress (1 mentions)
Sc 365230, by Santa Cruz Biotechnology (1 mentions)
Rabbit anti 4 hne antibody, by Bioss Antibodies (1 mentions)
Fer 1, by MedChemExpress (1 mentions)
Sc 166570, by Santa Cruz Biotechnology (1 mentions)
Liproxstatin 1, by MedChemExpress (1 mentions)
Glutathione (gsh), by MedChemExpress (1 mentions)
Erastin, by Selleck Chemicals (1 mentions)
5 protocols using sf1670
1
Cultivation of Gastric Cell Lines
2
Purchasing LXR-623 and SF1670 from MedchemExpress
3
Lipid Peroxidation Inhibitor Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Erastin, RSL-3, and necrostatin-1 (Ner-1) were purchased from Selleck Chemicals (Houston, USA). Tannic acid (TA), ferric chloride hexahydrate (FeCl3.6H2O), DMSO, and Ac-DEVD-CHO (Apo) were purchased from Macklin (Shanghai, China). EDTA and urea were purchased from Solarbio (Beijing, China). Fer-1, DFO, GSH, BSO, Liproxstatin-1, SF1670, and Chloroquine were purchased from MedChemExpress. Branched polyethyleneimine (PEI, Mw = 25,000) and 2′,7′-Dichlorofluorescin diacetate were purchased from Sigma-Aldrich. Antibodies against HA (MMS101P, Covance), FLAG (F1804, Sigma), UHRF1 (D6G8E, CST), HBP1 (11746-1-AP, Proteintech), CDO1 (12589–1 AP, Proteintech), PTEN (9188, CST), p-AKT (4060, CST), AKT (9272, CST), GPX4 (sc-166570, Santa cruz), ACSL4 (sc-365230, Santa cruz), TFRC (sc-32272, Santa cruz), β-actin (AC026, ABclonal). Rabbit anti-4-HNE antibody (Bioss). The following secondary antibodies were used: anti-mouse IgG antibody DyLight 800 (610-145-121, Rockland) and anti-rabbit IgG DyLight 800 (611-145-002, Rockland).
4
Investigating the Role of Nrf2 in NOD Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Non-obese diabetic (NOD) mice and Nrf2−/− mice were obtained from the Model Animal Research Center of Nanjing University (Nanjing, China). C57BL/6 mice were from Comparative Medical Center of Yangzhou University (Yangzhou, China). Experiments used 6–12-week-old and sex-matched mice, unless otherwise indicated. All animal care and handling procedures were conducted in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at Yangzhou University (Yangzhou, China).
The following reagents were used: Astilbin (HY-N0509); 1-aminobenzotriazole (ABT, HY-103389), a nonspecific and irreversible inhibitor of CYP enzymes; etomoxir (HY-50202), an inhibitor of CPT1α; SF1670 (HY-15842), an inhibitor of PTEN; N-Acetyl-L-cysteine (NAC, HY-B0215), a GSH-related antioxidant; GW9662 (HY-16578), a potent and selective PPARγ antagonist; and dorsomorphin (Compound C, HY-13418A), a selective and ATP-competitive AMPK inhibitor (MedChemExpress, NJ, United States).
The following reagents were used: Astilbin (HY-N0509); 1-aminobenzotriazole (ABT, HY-103389), a nonspecific and irreversible inhibitor of CYP enzymes; etomoxir (HY-50202), an inhibitor of CPT1α; SF1670 (HY-15842), an inhibitor of PTEN; N-Acetyl-L-cysteine (NAC, HY-B0215), a GSH-related antioxidant; GW9662 (HY-16578), a potent and selective PPARγ antagonist; and dorsomorphin (Compound C, HY-13418A), a selective and ATP-competitive AMPK inhibitor (MedChemExpress, NJ, United States).
5
Naringenin Effects on RCC Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RCC cells were cultured with naringenin (0, 4 or 8 µm) for different periods of time (0, 24, 48, 72 or 96 h), or, after pretreatment with a PTEN inhibitor (SF1670, MedChemExpress)/activator (Oroxin B, MedChemExpress) for 24 h in a 37°C CO2 incubator, cells were treated with 4 µm naringenin for 24 h. Subsequently, cells were incubated with 10 µl CCK-8 at 37°C for 2–4 h. Finally, cell proliferation was determined by measurement of the absorbance at 450 nm on a spectrophotometer.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!