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Migration or invasion chambers

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Migration or invasion chambers are lab equipment used to study the movement and behavior of cells. These chambers allow for the observation and quantification of cell migration or invasion through a barrier, such as a membrane or matrix. The core function of these chambers is to provide a controlled environment for the study of cellular processes related to movement and invasion.

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Countess, by Thermo Fisher Scientific (1 mentions)

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Invasion chambers (38 citations) Migration chambers (33 citations)

4 protocols using migration or invasion chambers

1

Cell Migration and Invasion Assay

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In brief, 200 μL cells (2×105 cells) resuspended with free medium, were added to the upper compartment of migration or invasion chambers (BD Biosciences). The bottom chamber was filled with 500 μL cell culture medium with 10% FBS as an attractant. After 24 h, cells were fixed and stained with Wright-Giemsa and counted at ×200 magnification in 10 randomly chosen fields. Experiments were repeated three times.
Chen L.Y., Wang L., Ren Y.X., Pang Z., Liu Y., Sun X.D., Tu J., Zhi Z., Qin Y., Sun L.N, & Li J.M. (2020). The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation. Molecular Cancer, 19, 164.
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2

Cell Migration and Invasion Assay

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Briefly, transfected U-CH1 and U-CH2 cells and their negative control were resuspended in serum-free medium. Then, 200 mL (5 × 104 cells) was seeded in the upper chambers of migration or invasion chambers (BD Biosciences). The bottom chamber was filled with 600 mL culture medium with 20% FBS. After 48 h, the cells in the upper chamber were removed with a swab, and the cells that migrated to the lower layer and attached to the membrane were stained with 0.1% crystal violet and were counted in five fields per well under a microscope. Experiments were repeated three times.
Zhang H., Yang K., Ren T., Huang Y., Tang X, & Guo W. (2018). miR-16-5p inhibits chordoma cell proliferation, invasion and metastasis by targeting Smad3. Cell Death & Disease, 9(6), 680.
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3

Radiation Response Modulation in MDA-MB-231 Cells

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MDA-MB-231 cells were plated as for the clonogenic assay. Cells were treated with 15 mM NAC and 100 µM BSO for 24 hours, 500 nM Au was added during the last hour of treatment and then culture dishes were irradiated with 2 Gy. The media was then refreshed and the plates were placed back in the incubator for 2.5 hours. Cells were then trypsinized and live cells were determined using the trypan blue assay via Countess (Invitrogen) and reseeded at 2.5 × 104 live cells in migration or invasion chambers (BD Biocoat) in serum free media. Media containing FBS was placed in the bottom chamber. After 16 hours incubation, non-invading cells were scrubbed from the top surface and the membranes stained with Giemsa violet. Cells were counted on four distinct quadrants of each chamber and averaged and the experiment was repeated at least four times.
Rodman SN I.I.I., Spence J.M., Ronnfeldt T.J., Zhu Y., Solst S.R., O’Neill R.A., Allen B.G., Guan X., Spitz D.R, & Fath M.A. (2016). Enhancement of Radiation Response in Breast Cancer Stem Cells by Inhibition of Thioredoxin and Glutathione Dependent Metabolism. Radiation research, 186(4), 385-395.
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4

Cell Migration and Invasion Assay

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PVNS FLS were treated according to the different experimental conditions in serum-free DMEM medium and seeded into the upper chamber of the migration or invasion chambers (BD Bioscience, California, USA) in 24-well cell culture plates (2 × 104 cells/well). The lower chamber contained DMEM medium with 10% FBS. After a 48 h incubation, the cells that had not penetrated the filter were removed from the top of the filter using cotton swabs, and the remaining cells were fixed in 4% (w/v) paraformaldehyde for 20 min, stained with 0.1% (w/v) crystal violet, and counted. Migration values were expressed as the number of migrated cells counted under a microscope. Three microscopic fields per membrane in triplicate experiments were counted. The cells lines used in each test were taken from three different patients, each patient's sample test was performed in triplicate.
Cao C., Wu F., Niu X., Hu X., Cheng J., Zhang Y., Li C., Duan X., Fu X., Zhang J., Zhang X, & Ao Y. (2020). Cadherin-11 cooperates with inflammatory factors to promote the migration and invasion of fibroblast-like synoviocytes in pigmented villonodular synovitis. Theranostics, 10(23), 10573-10588.
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