Log In Sign up
The largest database of trusted experimental protocols

Histamine

Manufactured by Beckman Coulter
Visit Supplier
Sourced in United States

Histamine is a laboratory equipment product used for the detection and quantification of histamine, a chemical compound involved in various physiological and pathological processes. This product provides a reliable and accurate method for analyzing histamine levels in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Rectal thermometer, by Physitemp (1 mentions) Cholera toxin, by List Biological Laboratories (1 mentions) Mmcp 1, by Thermo Fisher Scientific (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Histamine, by Merck Group (1 mentions) Immunotech im2562, by Beckman Coulter (1 mentions)

4 protocols using histamine

1

Peanut Allergy Induction and Challenge in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
4–6 week old female mice underwent weekly sensitization with 2 mg peanut extract and 10 μg Cholera toxin (List Biological laboratories, Campbell, CA) in 200 μL volume for three weeks by oral gavage followed by 1 week of 5 mg peanut extract and 10 μg Cholera toxin by oral gavage. One week after this final sensitization, mice were bled by submandibular bleed to collect serum for immunoglobulin quantification. The following day, mice undergoing an oral challenge were gavaged with 9 mg peanut extract while mice undergoing IP challenge received 200 μg peanut extract. Core body temperatures were monitored every fifteen minutes using a rectal thermometer (Physitemp, Clifton, NJ). For serum MMCP-1, histamine, and Ara h 1, 2, and 3 measurements, blood was collected 60 min after oral challenge. Serum levels of MMCP-1 (eBioscience, San Diego, CA), histamine (Beckman Coulter, Brea, CA) and Ara h 1, 2, and 3 (Indoor Biotechnologies, Charlottesville, VA) were measured by ELISA according to manufacturer’s instructions
Orgel K., Smeekens J.M., Ye P., Fotsch L., Guo R., Miller D.R., Pardo-Manuel de Villena F., Burks A.W., Ferris M.T, & Kulis M.D. (2018). Genetic Diversity Between Mouse Strains Allows Identification of CC027/GeniUnc as an Orally Reactive Model of Peanut Allergy. The Journal of allergy and clinical immunology, 143(3), 1027-1037.e7.
+ Open protocol
+ Expand
2

Quantification of Inflammatory Mediators

Check if the same lab product or an alternative is used in the 5 most similar protocols
After sensitization and stimulation of BMMCs with 100 ng/mL anti-DNP-IgE and DNP-HSA, respectively, the supernatants were collected and the amounts of MCPT1, IL-6, TNF-α (eBioscience), LTC4, PGD2 (Cayman, Ann Arbor MI, USA), and histamine (Beckman Coulter, Brea, CA, USA) were calculated using ELISA kits consistent with manufacturer's instructions. At 100 min following the DNP-HSA challenge in the PSA model, we collected serum samples from the mice, and the levels of histamine, MCPT1, and IL-6 were determined using ELISA.
Kim H.W., Ryoo G.H., Jang H.Y., Rah S.Y., Lee D.H., Kim D.K., Bae E.J, & Park B.H. (2022). NAD+-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice. Theranostics, 12(7), 3316-3328.
+ Open protocol
+ Expand
3

Histamine Degradation by rhDAO Variants

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Degradation of 100 ng/mL histamine (Sigma-Aldrich, St. Louis, MO, 53300) by 6 nM (1 µg/mL) rhDAO variants was measured in duplicate in phosphate buffered saline (PBS) with 1% human serum albumin (HSA; Albunorm; Octapharma, Vienna, Austria) or in EDTA plasma from a healthy volunteer, with or without 600 nM HMWH added (heparin, 1000 IU/mL equivalent to 2 mg/mL, Gilvasan, Vienna, Austria), using a Cisbio HTRF histamine 500 test kit (Biomedica, Vienna, Austria).
Plasma of seven mastocytosis patients and three healthy volunteers was spiked with 6 nM rhDAO-WT, 545 nM histamine, and 20 µM of the potent DAO-inhibitor diminazene aceturate (DIMAZ; Sigma-Aldrich, D7770) and histamine concentrations were determined in duplicate with a histamine ELISA (Immunotech IM2562, Beckman Coulter, Brea, CA) as described previously (Boehm et al., 2019 (link)).
Gludovacz E., Schuetzenberger K., Resch M., Tillmann K., Petroczi K., Schosserer M., Vondra S., Vakal S., Klanert G., Pollheimer J., Salminen T.A., Jilma B., Borth N, & Boehm T. (2021). Heparin-binding motif mutations of human diamine oxidase allow the development of a first-in-class histamine-degrading biopharmaceutical. eLife, 10, e68542.
+ Open protocol
+ Expand
4

Vitamin D Regulation of Mast Cell Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
BMCMCs and CBMCs or PBMCs were pre-incubated in 10% charcoal-stripped-FCS complete medium for 72 h, supplemented with the vitamin D3 metabolites and sensitized with IgE as outlined above for mast cell activation in vitro. Following the 16 h IgE-sensitization BMCMCs or CBMCs or PBMCs (106 cells/mL) were re-suspended in Tyrodes buffer and then activated with DNP-HSA (10 ng/ml; BMCMCs) or anti-human IgE Ab (1 μg/mL; CBMCs or PBMCs) for 30 min at 37°C in the presence of 1α,25(OH)2D3 (10−8 – 10−6 M) or 25OHD3 (10−8 – 10−6 M) or EtOH (0.03%). histamine or Cys-LT levels in supernatants and corresponding cell lysates (histamine only) were measured using histamine (Beckman Coulter) or Cys-LT EIA (Cayman Chemical) kits according to manufacturers’ instructions.
Yip K.H., Kolesnikoff N., Yu C., Hauschild N., Taing H., Biggs L., Goltzman D., Gregory P.A., Anderson P.H., Samuel M.S., Galli S.J., Lopez A.F, & Grimbaldeston M.A. (2014). Vitamin D3 represses IgE-dependent mast cell activation via mast cell-CYP27B1 and -vitamin D receptor activity. The Journal of allergy and clinical immunology, 133(5), 1356-136514.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!