Nfatc1 antibody

RANKL was purchased from PeproTech (London, UK). Dulbecco's modified Eagle's medium (DMEM) was purchased from Welgene (Daejeon, Korea). Cell medium minimum essential medium-α (α-MEM) and fetal bovine serum (FBS) were both purchased from Gibco (Gaithersburg, NY, USA). Penicillin/streptomycin was purchased from Invitrogen (Carlsbad, CA, USA). Dulbecco's phosphate-buffered saline (DPBS) was obtained from Gibco. An aqueous non-radioactive cell proliferation kit (for MTS assay) was purchased from Promega (Madison, WI, USA). A TRAP staining kit was purchased from Sigma-Aldrich (St. Louis, MI, USA). An Osteo Assay Stripwell plate was purchased from Corning Inc. (New York, NY, USA). We obtained the reverse transcription kit from Invitrogen. Taq polymerase was obtained from Kapa Biosystems (Woburn, MA, USA). PCR primers were from Genotech (Daejeon, Korea). Primary antibodies against c-Fos (Cat. no. sc-447) and β-actin (Cat. no. sc-8432) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA) and NFATc1 antibody (Cat. no. 556602) was obtained from BD Pharmingen (San Diego, CA, USA). Peroxidase IgG secondary antibody (Cat. no. 115-035-062) was purchased from Jackson ImmunoResearch (West Grove, PA, USA). Protease inhibitor cocktail and phosphatase inhibitor cocktail were both purchased from Sigma-Aldrich.