We preliminary tested 67 SSR markers that originated from mango. Of those, 21 were excluded because of no amplification, unstable amplification of the target band or the presence of monomorphic fragments. We used the remaining 46 SSR markers (Table 2 ), comprising 26 from Ravishankar et al. (2011) (link), 6 from Schnell et al. (2005) , and 14 from Viruel et al. (2005) .
SSR markers were amplified in a 5-μL reaction mixture, containing 2.5 μL of Multiplex PCR Master Mix with HotStar Taq DNA Polymerase (Qiagen), 5 pmol of each primer (forward, fluorescently labeled with FAM or HEX; R, unlabeled), and 5 ng of genomic DNA. The PCR profile consisted of initial denaturation for 15 min at 95°C; 35 cycles of denaturation for 60 s at 94°C, annealing for 60 s at 55°C, and extension for 60 s at 72°C; and a final extension for 7 min at 72°C. The amplified PCR products were separated and detected in a PRISM 3130xl DNA sequencer (Applied Biosystems, USA). The sizes of the amplified bands were scored against internal standard DNA (400HD-ROX, Applied Biosystems) in GeneScan software (Applied Biosystems).
SSR markers were amplified in a 5-μL reaction mixture, containing 2.5 μL of Multiplex PCR Master Mix with HotStar Taq DNA Polymerase (Qiagen), 5 pmol of each primer (forward, fluorescently labeled with FAM or HEX; R, unlabeled), and 5 ng of genomic DNA. The PCR profile consisted of initial denaturation for 15 min at 95°C; 35 cycles of denaturation for 60 s at 94°C, annealing for 60 s at 55°C, and extension for 60 s at 72°C; and a final extension for 7 min at 72°C. The amplified PCR products were separated and detected in a PRISM 3130xl DNA sequencer (Applied Biosystems, USA). The sizes of the amplified bands were scored against internal standard DNA (400HD-ROX, Applied Biosystems) in GeneScan software (Applied Biosystems).