Immune cells from BAL fluid or lung tissue cells were stained for 20 min at 4°C with Biotin rat anti‐mouse CD45 (clone 30‐F11; BD). Thereafter, cells were labelled for 20 min at 4°C with APC‐A750 Streptavidin (BD), APC rat anti‐mouse CD11c (clone N418; Thermo Fisher Scientific), APC‐A700 rat anti‐mouse CD11b (clone M1/70, Thermo Fisher Scientific), FITC rat anti‐mouse Ly6G (clone RB6‐8C5; Thermo Fisher Scientific), PE rat anti‐mouse Siglec F (clone E50‐2440, BD), PE‐CF594 rat anti‐mouse CD3 (clone 145‐2C11; BD), CD19 (clone 1D3, BD) and V500 IA/IE (clone M5/114.15.2; BD). The gating strategy is depicted in Figure S1 .
Pe rat anti mouse siglec f clone e50 2440
Manufactured by BD
PE rat anti‐mouse Siglec F (clone E50‐2440) is a flow cytometry antibody that binds to the Siglec F receptor on the surface of mouse cells. Siglec F is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family and is expressed on eosinophils and alveolar macrophages. This antibody can be used to identify and quantify Siglec F-positive cells.
Automatically generated - may contain errors
2 protocols using pe rat anti mouse siglec f clone e50 2440
1
Multiparametric Flow Cytometry of Lung Immune Cells
2
Multiparameter Flow Cytometry Profiling
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