HT29 cells were cultured overnight in 96-well plates (3.0×103 cells per well) and then treated with various concentrations of compounds in the normal or glucose-free medium for 48 hours. Then the medium was replaced with fresh normal medium, and the cells were cultured for a further 16 hours. Subsequently, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich) solution (0.5 mg/mL) was added to the culture medium. After a 4-hour incubation at 37°C, the medium was removed and DMSO was added (100 μL/well); then, absorbance of each well was determined at 570 nm by Multiskan JX plate reader (Thermo Scientific, Waltham MA, USA). Relative cell survival (mean ± standard deviation [SD] of triplicate determinations) was calculated by setting each of the control absorbance from nondrug-treated cells at 100%.
Multiskan jx plate reader
Manufactured by Thermo Fisher Scientific
Sourced in United States, Finland
The Multiskan JX plate reader is a compact, flexible, and easy-to-use instrument designed for absorbance-based measurements in microplates. It can accommodate a wide range of sample types and read 6- to 384-well plates with high precision and reproducibility.
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Lab products found in correlation
2 protocols using multiskan jx plate reader
1
MTT Cell Viability Assay in HT29 Cells
2
Cytokine Profiling in Mouse Samples
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Mouse interferon-γ (IFN-γ), IL-2, IL-6Rα, and TNF-α enzyme-linked immunosorbent assay (ELISA) kits (Quantikine) were purchased from R&D Systems (Minneapolis, MN, USA), a mouse Gp130 ELISA kit was purchased from RayBiotech (Peachtree Corners, GA, USA), and a mouse CCL8 ELISA kit was purchased from IBL (Takasaki, Japan) and used in accordance with the manufacturers' instructions. The plates were read using a Multiskan JX plate reader (Thermo Labsystems, Helsinki, Finland) . A cytometric bead array (BD, Tokyo, Japan) was used to measure Th1/Th2/Th17 cytokines, which included IL-2, IL-4, IL-6, IFN-γ, TNF-α, IL-17A, and IL-10, in accordance with manufacturer's instructions.
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