Heparin

Manufactured by STEMCELL

Sourced in Canada, United States, China, Israel, France

Heparin is a naturally occurring anticoagulant used in various laboratory applications. It is a glycosaminoglycan that inhibits several steps in the blood coagulation cascade, primarily by enhancing the activity of antithrombin III. Heparin is commonly used in cell culture media and other lab procedures to prevent the formation of blood clots.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Heparin solution (31 citations) Heparin sulfate (4 citations) Heparin sodium salt (4 citations) Heparin Sodium Salt in PBS (3 citations)

274 protocols using heparin

Differentiation of ReNcell VM into Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immortalized hNPC cell line ReNcell VM (ReN) was used to differentiate into neurons per published protocol.16 Simply, ReN cells were grown in proliferation medium (DMEM/F12; Gibco/Life Technologies, Grand Island, NY) with 2 µg/ml of heparin (stock; STEMCELL Technologies, Cambridge, MA), 2% B27 (Life Technologies, Carlsbad, CA), 20ng/ml of epidermal growth factor (EGF), 20ng/ml of basic fibroblast growth factor, and 1% of 100 × penicillin/streptomycin (Gibco/Life Technologies) until confluency. For differentiation, 3 × 10⁴ cells were seeded on Matrigel‐coated (1:100 dilution in DMEM/F12) eight‐well chamber slides and grown in differentiation medium (DMEM/F12; Gibco/Life Technologies) with 2 µg/ml of heparin (stock; STEMCELL Technologies), 2% B27 (Life Technologies), and 1% of 100 × penicillin/streptomycin (Gibco/Life Technologies) for 2 to 3 weeks before coculture with microglia.

Gong Y., Sasidharan N., Laheji F., Frosch M., Musolino P., Tanzi R., Kim D.Y., Biffi A., El Khoury J, & Eichler F. (2017). Microglial dysfunction as a key pathological change in adrenomyeloneuropathy. Annals of Neurology, 82(5), 813-827.

+ Open protocol

+ Expand

Primitive Streak to Kidney Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

iPSCs were induced to differentiate toward the primitive streak by treating cells with 7 μM CHIR99021(STEMCELL Technologies, Catalog # 72052) in TeSR-E6(STEMCELL Technologies, Catalog # 05946) medium for 4 days. Next, 200 ng/ml FGF9(MCE, Catalog # HY-P7177), 1 μg/ml heparin(STEMCELL Technologies, Catalog # 07980) and 1 μM CHIR99021 were added to induce the iPSCs to differentiate toward intermediate mesoderm (IM) for 3 days. The IM cells were digested into single cells, resuspended in 200 ng/ml FGF9, 1 μg/ml heparin, 1 μM CHIR99021, 0.1% PVA, 0.1% MC, and 10 μM ROCK inhibitor(STEMCELL Technologies, Catalog # 72308) medium, and cultured in a horizontal shaker. After 24 hours, the ROCK inhibitor was removed from the medium. On the next five days, all cytokines were removed and maintained in TeSR-E6 medium. Organoids were spontaneously formed in the following 13 days.

Cheng C., Wang G., Zhu Y., Wu H., Zhang L., Liu Z., Huang Y, & Zhang J. (2024). Multiplexed bulk and single-cell RNA-seq hybrid enables cost-efficient disease modeling with chimeric organoids. Nature Communications, 15, 3946.

+ Open protocol

+ Expand

Isolation and Culture of ENCDCs from Wnt1 Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

ENCDCs for cell transplantation were isolated from the small intestinal LMMP layer of Wnt1^tdT/+ mice. After the mice were euthanized, the small intestine was collected and placed in ice-cold PBS. LMMP was separated from the underlying submucosa using fine forceps, then finely minced into 1 mm pieces and enzymatically dissociated with dispase (250 μg/mL; StemCell Technologies, Vancouver, BC) and collagenase XI (1 mg/mL; Sigma Aldrich, St. Louis, MO) at 37 °C for 40 minutes. Single cells were isolated by filtration through a 40 μm filter and plated at 50,000 cells/mL in a 25-cm² flask in mouse proliferation media, consisting of Neurocult Mouse Basal Medium (StemCell Technologies) supplemented with 10% Neurocult Mouse Proliferation Supplement (StemCell Technologies), 20 ng/mL epidermal growth factor (StemCell Technologies), 10 ng/mL basic fibroblast growth factor (StemCell Technologies), 0.0002% Heparin (StemCell Technologies) and antibiotics (penicillin and streptomycin). After 7 days, primary cell aggregates (neurospheres) were obtained. Neurospheres from Wnt1^tdT/+ mice were used for cell transplantation.

Bhave S., Arciero E., Baker C., Ho W.L., Stavely R., Goldstein A.M, & Hotta R. (2019). Enteric neuronal cell therapy reverses architectural changes in a novel diphtheria toxin-mediated model of colonic aganglionosis. Scientific Reports, 9, 18756.

+ Open protocol

+ Expand

Inhibition of Key Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following inhibitors were used, as indicated: MMP inhibitors - BB94 (Selleckchem cat#S7155; 20 nM), AG3340 (Sigma cat#PZ0198; 10μM), NFkB inhibitor—Bay11-7082 (Selleckchem cat#S2913; 0.5 μM), NOTCH inhibitor—DAPT (Sigma cat#D5942; concentration as per supplementary Fig. 10), Wnt inhibitor—LGK-974 (Selleckchem cat#S7143; 2 μM), TGFb pathway inhibitor—LY2157299 (Selleckchem cat#S2230; 10 μM), Proteoglycan EV receptor inhibitor—Heparin (Stemcell Technologies cat#7980, 10 μg/ml).

Adnani L., Kassouf J., Meehan B., Spinelli C., Tawil N., Nakano I, & Rak J. (2022). Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells. Nature Communications, 13, 5494.

+ Open protocol

+ Expand

Culturing diverse cancer cell lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

U87-MG, LN-229, and HT-29 cells were obtained from America Type Culture Collection, (ATCC #HTB-14, CRL-2611, and HTB-38, respectively). IGROV-1 and HEY-A8 were obtained from the NCI-60 panel of human cancer cell lines [43 (link)]. U87-MG, LN-229, HT-29, and HEY-A8 cells were cultured in Dulbecco's Modified Eagle Medium (DMEM; Life Technologies) supplemented with 10% fetal bovine serum (FBS; HyClone), 100 U/ml penicillin and 100 μg/ml streptomycin (referred to as 1% Pen/Strep; Life Technologies). IGROV-1 cells were cultured in RPMI-1640 (Life Technologies), supplemented with 2mM L-Glutamine (Life Technologies), 10% FBS and 1% Pen/Strep. Generation and characterization of JHH-136 and JHH-520 cell lines has been previously described [28 (link)]. Both cell lines were grown in suspension in Neurocult NS-A Stem Cell media (StemCell Technology), containing 20 ng/ml human EGF (Peprotech), 10 ng/ml human basic FGF (Peprotech) and 0.2% heparin (StemCell Technology). TSCER2 cells, derived from the human lymphoblast cell line TK6 [44 (link)], were cultured in RPMI 1640 medium (Life Technologies) supplemented with 5% FBS (Gemini Bio-Products), 1 mM sodium pyruvate (Life Technologies) and 1% Pen/Strep.
All cultures were maintained in a 37°C incubator with 5% CO₂ and under a humidified atmosphere.

Martinez N.J., Rai G., Yasgar A., Lea W.A., Sun H., Wang Y., Luci D.K., Yang S.M., Nishihara K., Takeda S., Sagor M., Earnshaw I., Okada T., Mori K., Wilson K., Riggins G.J., Xia M., Grimaldi M., Jadhav A., Maloney D.J, & Simeonov A. (2016). A High-Throughput Screen Identifies 2,9-Diazaspiro[5.5]Undecanes as Inducers of the Endoplasmic Reticulum Stress Response with Cytotoxic Activity in 3D Glioma Cell Models. PLoS ONE, 11(8), e0161486.

+ Open protocol

+ Expand

Orthotopic Transplantation of EGFRvIII-Expressing Neurospheres

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumors were also generated by orthotopic transplantation of EGFRvIII-expressing neurospheres, which were produced in vitro. Subventricular zones (SVZs) were dissected from P0-P2 Cdkn2a^−/−; EGFRvIII^fl-stop-fl;Pten^fl/fl mice and dissociated into single cells with Accutase (Biolegend, 423,201). The cells were cultured in serum free neural stem cell media containing 1× B27 supplement (Life Technologies, 17604044), 20 ng/ml b-FGF (ThermoFisher, PHG0261), 5 μg/ml heparin (Stem Cell Technologies, 7980), 25 ng/ml EGF (Lonza, cc-4017FF) and 1 X penicillin/streptomycin (Genesee, 25512) in DMEM: F12 medium (Gibco, 10565018) (Laks et al., 2009 (link)). The cells from individual animals were initially plated in 6-well plates to grow neurospheres over 1–2 weeks. 500,000 cells were then plated in 6-well plates and treated with concentrated adenovirus Cre (Vector Biolabs) at 50–100 MOI, and then cultured in 10 cm dishes for another 5–7 days before they were checked by western blot to confirm EGFRvIII expression and loss of PTEN protein expression. They were passaged for less than 8 passages before being injected into 6-week old C57BL/6 mice to form tumors. About 200,000 cells in 5 μl 1× DPBS were injected per animal using a 23-gauge Hamilton syringe at a position 2.5 mm to the right and 1 mm anterior to the bregma at a depth of 2.5–3 mm.

Chen Z., Herting C.J., Ross J.L., Gabanic B., Vallcorba M.P., Szulzewsky F., Wojciechowicz M.L., Cimino P.J., Ezhilarasan R., Sulman E.P., Ying M., Ma’ayan A., Read R.D, & Hambardzumyan D. (2020). Genetic driver mutations introduced in identical cell-of-origin in murine glioblastoma reveal distinct immune landscapes but similar response to checkpoint blockade. Glia, 68(10), 2148-2166.

+ Open protocol

+ Expand

Culturing Neural, Cancer, and Fibroblast Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Neural stem cell cultures have been previously reported by us [16 (link), 17 ]. U87 and HeLa cells were maintained in DMEM high-glucose (HyClone #SH30081.02). HSVT-C3 and BTIC-13 cells were maintained in medium DMEM/F-12 (GIBCO #11320033). Primary neural stem cells were maintained in proliferation medium (Stem Cell Technologies) supplemented with epidermal growth factor (EGF; 20 ng/mL), fibroblast growth factor (FGF; 10 ng/mL) and heparin (10 ng/mL) (Stem Cell Technologies). LNCaP, C4-2B, 22Rv1, DU-145 and ZR-75-30 cells were maintained in medium RPMI 1640 (HyClone #SH30255.02). Human dermal fibroblasts (HDF) were cultured in MEM (HyClone #SH30024.02). All cells were supplemented with 10% FBS, except HSVT-C3 and HDF cells which were supplemented with 5% FBS (Mediatech, #MD.35-010-CV), GlutaMAX (GIBCO # 35050061) and 100 U/mL penicillin-streptomycin (Corning # 30-002-CI). To ensure mycoplasma-free cultures, the cells were treated with BM cyclin (Roche, #10799050001). Cells were maintained at 37 °C, 5% v/v CO2 in a humidified incubator. Importantly, all cells were used with passage no greater than 30 or 35, because aged cultures showed dissimilar responses and increased sensitivity to ferroptosis inducers such as IKE and RSL-3.

Ferrada L., Barahona M.J., Vera M., Stockwell B.R, & Nualart F. (2023). Dehydroascorbic acid sensitizes cancer cells to system xc- inhibition-induced ferroptosis by promoting lipid droplet peroxidation. Cell Death & Disease, 14(9), 637.

+ Open protocol

+ Expand

Neurosphere Generation from Neural Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To generate neurospheres, acutely isolated NSPCs were cultured at a density of 10⁵ cells/mL in fetal growth media (FGM) consisting of X-VIVO 15 media (Lonza, cat. #04–744Q) supplemented with N-2 (ThermoFisher Scientific, cat. #17502048), heparin (STEMCELL Technologies, cat. #07980), N-acetylcysteine (VWR, cat. #E-3710), 20 ng/mL fibroblast growth factor 2 (FGF2) (Shenandoah Biotechnology, cat. #100–146), 20 ng/mL epidermal growth factor (EGF) (Shenandoah Biotechnology, cat. #100–26), and 10 ng/mL leukemia inhibitory factor (LIF) (Sigma, cat. #LIF1010). No established cell lines were used in this study.

Liu D.D., He J.Q., Sinha R., Eastman A.E., Toland A.M., Morri M., Neff N.F., Vogel H., Uchida N, & Weissman I.L. (2023). Purification and characterization of human neural stem and progenitor cells. Cell, 186(6), 1179-1194.e15.

+ Open protocol

+ Expand

Mammosphere Formation and Proliferation Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For mammosphere assay, single-cell suspensions were cultured at 5% O₂ and 21% O₂ to form mammospheres in an ultralow-attachment six-well plate at a density of 5000 cells per well in MammoCult basal medium supplemented with MammoCult proliferation supplement (#05620), heparin (#07980) from STEMCELL Technologies Inc., hydrocortisone (#H0888, Sigma-Aldrich), and penicillin/streptomycin (#30-002-CI, Corning) as described previously (55 (link)). Phase-contrast images were captured, and mammospheres were trypsinized and processed for staining at day 5. For cell proliferation assay, cells were seeded at the indicated density in 96-well plates and grown for 2, 4, and 6 days. At the end of time points, proliferation rates were determined using CellTiter-Glo luminescent cell viability assay (G7571, Promega) according to the manufacturer’s instructions.

Kumar B., Adebayo A.K., Prasad M., Capitano M.L., Wang R., Bhat-Nakshatri P., Anjanappa M., Simpson E., Chen D., Liu Y., Schilder J.M., Colter A.B., Maguire C., Temm C.J., Sandusky G., Doud E.H., Wijeratne A.B., Mosley A.L., Broxmeyer H.E, & Nakshatri H. (2022). Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity. Science Advances, 8(2), eabh3375.

+ Open protocol

+ Expand

DIPG Cell Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary DIPG cultures were grown mainly as neurospheres in all the experiments listed in this study. All cultures have been validated by STR profiling and mycoplasma testing. DIPG cells were use within the following passage ranges HSJD-DIPG007—p36–37, SU-DIPGVI—p29-p41, RA055—P2-P5 and VUMC-DIPG10—P3-P6. DIPG cultures were grown in stem cell media containing half quantity of DMEM/F12 and half of the Neurobasal medium (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA) supplemented with glutamax, pyruvate, non-essential amino acids, HEPES buffer, antibiotic/antimycotic (Invitrogen). Stem cell media were freshly supplemented each time with heparin (Stem Cell Technologies, Vancouver, BC, Canada), human EGF, human basic FGF, PDGF-AA and PDGF-BB (Stem Cell Technologies) [44 (link)]. Cultures were maintained at 37° C in a humidified atmosphere with 5% CO₂.

Ung C., Tsoli M., Liu J., Cassano D., Pocoví-Martínez S., Upton D.H., Ehteda A., Mansfeld F.M., Failes T.W., Farfalla A., Katsinas C., Kavallaris M., Arndt G.M., Vittorio O., Cirillo G., Voliani V, & Ziegler D.S. (2021). Doxorubicin-Loaded Gold Nanoarchitectures as a Therapeutic Strategy against Diffuse Intrinsic Pontine Glioma. Cancers, 13(6), 1278.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!