Serum samples were collected in the acute phase of the disease at a late time point (median 21 days, IQR 18–33) from the onset of the disease. Blood samples were collected into heparinized vacutainer tubes (Sarstedt, Nümbrecht, Germany #04.1927). Blood plasma samples were obtained by centrifugation, aliquoted into collection vials and stored at −70 °C. Samples were named according to the patient IDs and tested using six serological assays (Supplementary Figure S1 ).
Heparinized vacutainer tubes
Manufactured by Sarstedt
Sourced in Germany
Heparinized vacutainer tubes are laboratory equipment used for the collection and storage of blood samples. They contain the anticoagulant heparin, which prevents the blood from clotting during the collection and storage process. The primary function of these tubes is to facilitate the collection and preservation of blood samples for various analytical and diagnostic purposes in a clinical or research laboratory setting.
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Lab products found in correlation
2 protocols using heparinized vacutainer tubes
1
Acute Phase Serum Collection Protocol
2
PBMC Isolation and B-cell Purification
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Whole-blood samples were collected into heparinized vacutainer tubes (Sarstedt, Nümbrecht, Germany). Peripheral blood mononuclear cells (PBMCs) were isolated via density gradient centrifugation. Plasma samples were stored at −70 °C. B cells were purified from freshly isolated PBMCs by negative selection using the Dynabeads Untouched human B-cells kit (Thermo Fisher Scientific, Waltham, MA, USA) and were used in the assays immediately. As a pre-pandemic control, we used blood samples cryopreserved no later than September 2019.
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