HEK293T cells were obtained from the JCRB Cell Bank (Osaka, Japan) and maintained in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum at 37 °C with 95% air and 5% CO2.
Hek293t cells
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
HEK293T cells are a widely used human embryonic kidney cell line that is commonly employed in various research applications. These cells are known for their ability to efficiently express recombinant proteins and are often utilized in the development and testing of viral vectors, gene expression studies, and protein production.
Automatically generated - may contain errors
Lab products found in correlation
Molm 13, by Leibniz Institute DSMZ (3 mentions)
Mv4 11, by Leibniz Institute DSMZ (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Skno 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Nomo 1, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Oci aml2, by Leibniz Institute DSMZ (1 mentions)
Dulbecco s modified eagle s medium, by Fujifilm (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
5 protocols using hek293t cells
1
HEK293T Cell Culture Protocol
2
Cell Line Cultivation Protocols
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AML-derived THP-1 and KG-1a cells were purchased from RIKEN biological resource center (BRC, Japan). AML-derived Kasumi-1, HL60, SKNO-1, NOMO-1 cells and embryonic kidney derived HEK293T cells were from Japanese Collection of Research Bioresources (JCRB, Japan). AML-derived KO52, NB4, ME-1, ML-2, OCI-AML2, MV4-11, MOLM-13 and HEL cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). HEK293T cells were maintained in Dulbecco's modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Penicillin–Streptomycin (PS) in a humidified incubator with 5% CO2 and 95% air at 37 °C. The other cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
3
Culturing HEK293T Cells for Research
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HEK293T cells were obtained from the JCRB Cell Bank (Osaka, Japan) and maintained in Dulbecco’s modified Eagle’s medium (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) supplemented with 2 mM glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 10% fetal bovine serum (Sigma-Aldrich, St Louis, MO, USA) at 37 °C with 95% air and 5% CO2.
4
Establishment and Maintenance of AML Cell Lines
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AML-derived MOLM13 and MV4-11cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ), Germany and American Type Culture Collection (ATCC), USA, respectively. AML-derived MV4-11NR cells harboring TP53 R248W mutation were kind gift from Dr. T. Ikezoe (Department of Hematology and Respiratory Medicine, Kochi University, Kochi, Japan). Embryonic kidney HEK293T cells were provided from Japanese Collection of Research Bioresources (JCRB), Japan. HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 1% Penicillin-Streptomycin (PS) in incubators with humidified atmospheres of 5% CO2 and 95% air at 37 °C. The other cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium containing 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
5
Cell Culture of MLL-r AML and HEK293T
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MLL‐r AML‐derived MOLM‐13 and MV4‐11 cells were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Braunschweig, Germany), and American Type Culture Collection (ATCC, Manassas, VA, USA), respectively. These MLL‐r AML cell lines were cultured in Roswell Park Memorial Institute 1640 medium containing 10% heat‐inactivated FBS and 1% penicillin–streptomycin (PS) under 5% CO2 and 95% air at 37 °C. Embryonic kidney HEK293T cells were obtained from Japanese Collection of Research Bioresources (JCRB, Ibaraki, Osaka, Japan). HEK293T cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% FBS and 1% PS under 5% CO2 and 95% air at 37 °C.
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