Moflo xdp sorter

Manufactured by Beckman Coulter

Sourced in United States

The MoFlo XDP sorter is a high-performance cell sorting instrument designed for advanced flow cytometry applications. It features a robust and flexible platform that enables precise cell sorting and analysis. The MoFlo XDP sorter provides reliable and efficient sorting capabilities to support a wide range of research and clinical laboratory workflows.

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Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (3 mentions) Cd34 efluor 660, by Thermo Fisher Scientific (3 mentions) Facscanto 2, by BD (3 mentions) Alpha6 pe, by Thermo Fisher Scientific (2 mentions) Facscalibur, by BD (2 mentions) Rnaprotect, by Qiagen (2 mentions) Miseq platform, by Illumina (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) α mem medium, by Thermo Fisher Scientific (2 mentions) Effectene reagent, by Qiagen (2 mentions) Anti cd44 fitc, by Thermo Fisher Scientific (2 mentions) 7 aminoactinomycin d 7 aad, by BioLegend (1 mentions) Recombinant ribonuclease inhibitor, by Takara Bio (1 mentions) Summit 5, by Beckman Coulter (1 mentions) 293t cell line, by American Type Culture Collection (1 mentions) Nk cell isolation kit 2, by Miltenyi Biotec (1 mentions) Cd4 pe, by BD (1 mentions) Facsvantage, by BD (1 mentions) Cd3 fitc, by BD (1 mentions) Igg1 fitc, by BD (1 mentions)

Spelling variants of this product

MoFlo XDP (580 citations) MoFlo XDP cell sorter (304 citations) MoFlo XDP High-Speed Cell Sorter (22 citations) MoFlo sorter (20 citations) MoFlo™ XDP High-Performance Cell Sorter (12 citations) MoFlo XDP Flow Cytometer/Sorter (5 citations) MoFlo XDP high-speed sorter (2 citations) MoFlo XDP cell sorter/ Flow cytometer (2 citations) MoFLo XDP cell sorter machine (2 citations) MoFlo XDP high-speed flow cytometry sorter (1 citations)

47 protocols using moflo xdp sorter

Isolation of Adipose-Derived Cell Populations

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SVF cells from subcutaneous adipose tissue were resuspended in Hanks’ balanced salt solution buffer for incubation with CD45 antibody (1:200; BioLegend, #25-0451-82, 30-F11) for 30 min at 4 °C. 7-Aminoactinomycin D (7-AAD; 1:1,000; BioLegend, #420404) was added 10 min before fluorescence-activated cell sorting (FACS). The cells were sorted with MoFlo XDP sorter (Beckman Coulter) with a 100-μm nozzle. For isolation of primary adipocytes nuclei, purified nuclei from adipocytes were resuspended in PBS supplemented with recombinant ribonuclease inhibitor (0.5 U/μl; Takara, #2313B). 7-AAD (1:1,000) was added 10 min before FACS. The nuclei were sorted with MoFlo XDP sorter (Beckman Coulter) with a 100-μm nozzle.

Liu Q., Long Q., Zhao J., Wu W., Lin Z., Sun W., Gu P., Deng T., Loomes K.M., Wu D., Kong A.P., Zhou J., Cheng A.S, & Hui H.X. (2023). Cold-Induced Reprogramming of Subcutaneous White Adipose Tissue Assessed by Single-Cell and Single-Nucleus RNA Sequencing. Research, 6, 0182.

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Hair Follicle Isolation and Sorting

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WT mice, EZH2^-/- or STK40^-/- (9 days postnatal) were anesthetized and the hair on the back was shaved with electric scissors to avoid mutilation to the skin and subcutaneous tissues. Then, 70% ethanol was applied for disinfection and removal of the remaining hair residues. The entire skin was dissected and immersed in trypsin (GIBCO, Carlsbad, CA, USA) with the dermis facing down at 4°C O/N. Single-cell suspension was subsequently prepared by dissociating the epidermis and HF from the dermis. The cells were rinsed using phosphate buffer saline (PBS) containing 5% fetal bovine serum (FBS), and filtered using a 70 μm and then 40 μm cell strainer, respectively. The cell suspension was incubated with the experimental antibody for 90 min on ice. The antibodies used were as follows: Alpha6-PE (1 : 500; eBioscience, San Diego, CA, USA) and CD34-eFluor660 (1 : 100, eBioscience). Dead cells were eliminated using 4', 6-diamidino-2-phenylindole (DAPI). MoFlo XDP sorters (Beckman Coulter Inc., Brea, CA, USA) equipped with the Summit 5.2 software were adopted for definitive cell isolation [6 (link)].

Cai B., Li M., Zheng Y., Yin Y., Jin F., Li X., Dong J., Jiao X., Liu X., Zhang K., Li D., Wang J, & Yin G. (2020). EZH2-mediated inhibition of microRNA-22 promotes differentiation of hair follicle stem cells by elevating STK40 expression. Aging (Albany NY), 12(13), 12726-12739.

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Isolation of Skin Cell Populations

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Subcutaneous fat was removed by a scalpel and the whole skin was placed dermis side down in Trypsin (GIBCO) at 4°C O/N. Single-cell suspensions were obtained by scraping the skin to remove the epidermis and HFs from the dermis. The cells were washed with PBS containing 5% of fetal bovine serum (FBS), then filtered through 70 μm cell strainers, followed by 40 μm. Cell suspensions were incubated with the appropriate antibodies for 90 min on ice. The following antibodies were used: Alpha6-PE (1:500, eBioscience), CD34-eFluor660 (1:100, eBioscience). DAPI was used to exclude dead cells. Cell isolations were performed on MoFlo XDP sorters equipped with Summit 5.2 software (Beckman Coulter).

Yuan S., Li F., Meng Q., Zhao Y., Chen L., Zhang H., Xue L., Zhang X., Lengner C, & Yu Z. (2015). Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genetics, 11(5), e1005253.

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Isolation and Characterization of T Cell Subsets

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Seventy-four healthy blood donors were recruited under NIH IRB approved protocols and all donors provided written informed consent regarding their participation in the study. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from blood by Ficoll-Hypaque density gradient centrifugation (Supplementary Table 1). Cells were stained for sorting with anti-CD8, anti-CD4, anti-CD62L, and anti-CD45RA. MoFlo XDP Sorter (Beckman Coulter) was used to sort CD45RA⁺ CD62L⁺ naïve cells (T_N), CD45RA^- CD62L⁺ central memory (T_CM) cells, and CD45RA^- CD62L^- effector memory (T_EM) cells. All cells were maintained at 37 °C and 5% CO₂. The human leukemic T-cell line Jurkat, subclone E6 (ATCC), was maintained in standard growth medium (RPMI 1640 medium supplemented with 10% fetal bovine serum) while 293T cell line (ATCC) was cultured in DMEM/HG media with 10% FBS. Passages 5-15 were used in this study.

Yang Q., Patrick M., Lu J., Chen J., Zhang Y., Hemani H., Lehrmann E., De S, & Weng N.P. (2024). Homeodomain-only protein suppresses proliferation and contributes to differentiation- and age-related reduced CD8+ T cell expansion. Frontiers in Immunology, 15, 1360229.

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Neurosphere Formation and Expansion

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Neurospheres at passage 3–8 were dissociated using Accutase^® and sorted at one cell per well of 96-well round bottom, uncoated tissue culture plates. Forward scatter/side scatter criteria and Calcein Blue AM were used on the MoFLO™ XDP sorter (Beckman Coulter Life Sciences) to sort single live cells. Half of the wells were treated GDF11 (50 ng/ml), and the other half were treated with vehicle (4 mM HCl, 0.1% BSA, 0.5X DPBS). Media was changed to replace growth factors and ligands every 3–4 days. After 10 days in vitro, wells were assessed for the presence of spheres, and the number of cells per sphere was determined by bright field microscopy and staining with Hoechst 33342. Average colony size was compared between vehicle and GDF11-treated conditions.

Ozek C., Krolewski R.C., Buchanan S.M, & Rubin L.L. (2018). Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice. Scientific Reports, 8, 17293.

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Isolation and Activation of Murine NK Cells

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NK cells were enriched from total splenocytes by negative magnetic separation using NK cell isolation kit II (MACS Miltenyi Biotec) according to the manufacturer's instructions. Total splenocytes were labeled with α-NK1.1, α-TCRβ, and α-CD19 (to obtain T cells and B cells), while total hepatic leukocytes (to obtain NKT cells) and enriched NK cells were labeled with α-NK1.1 and α-TCRβ. The labeled cells were then flow sorted by using MoFlo XDP-sorter from Beckman Coulter (Stem Core laboratories, OHRI, Ottawa) to obtain T cells, B cells, NK cells, and NKT cells. The purified NK cells were stimulated ex vivo with rhIL-2 (1,000 U/ml) and IL-12 (50 ng/ml) in RP-10 medium for 18 h at 37°C and the conditioned media was harvested to measure IL-10 secretion. The purity of the fraction was >95%.

Ali A.K., Komal A.K., Almutairi S.M, & Lee S.H. (2019). Natural Killer Cell-Derived IL-10 Prevents Liver Damage During Sustained Murine Cytomegalovirus Infection. Frontiers in Immunology, 10, 2688.

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EBV-Induced B Cell Proliferation

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PBMCs were isolated from a buffy coat and stained with CTV using the manufacturer’s suggested protocol (Invitrogen; #C34557) followed by infection with EBV at a multiplicity of infection (MOI) of 5 (such that all infected B cells are positive for EBNA-LP). Proliferation was monitored in CD19⁺ B cells by the dilution of the CTV stain for up to 14 days post infection on a BD FACS Canto II (Becton Dickinson, Franklin Lakes, NJ, USA) and analyzed using FlowJo 10.0 software (TreeStar) (FlowJo, Ashland, OR, USA). CD19-positive cells were sorted into early and late population doublings based upon their CTV profile using either a Beckman Coulter Astrios or Beckman Coulter MoFlo XDP sorter (Beckman Coulter, Brea, CA, USA). Sorting to capture early proliferating and late proliferating populations were conducted as follows:

Hafez A.Y., Messinger J.E., McFadden K., Fenyofalvi G., Shepard C.N., Lenzi G.M., Kim B, & Luftig M.A. (2017). Limited nucleotide pools restrict Epstein–Barr virus-mediated B-cell immortalization. Oncogenesis, 6(6), e349-.

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Generation of Isogenic HAP1 Cell Lines

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Near-haploid, HAP1 human cells were obtained directly from Horizon Discovery and cultured according to standard cell culture protocols in Iscove’s Modified Dulbecco’s Medium (IMDM), supplemented with 10% FBS and 1% PenStrep. Isogenic cell lines deficient for the human homologs of yeast instability genes (ATG2B, SMARCA4, DYRK1B, GRID2IP, DDHD1) were generated using CRISPR/Cas9-mediated inactivation. In summary, single-guide RNAs (sgRNAs) were designed against the target genes (Extended Data Fig. 8c), while attempting to maximize on-target activity and minimizing off-target activity^{26 (link)}. Individual sgRNAs were cloned into a spCas9- and GFP-expressing plasmid (pX458)^{27 (link)} and transfected into wildtype HAP1 cells using Lipofectamine 3000. Individual clones were generated from single GFP-positive cells isolated by FACS (MoFlo XDP Sorter, Beckman Coulter). Sanger sequencing was used to identify isogenic clones for each target gene, carrying small frameshift insertions/deletions in early exons, which were predicted to generate no active protein products (Extended Data Fig. 8c). The ΔMSH2 and ΔFRYL HAP1 lines were obtained directly from Horizon Discovery, while the ΔMUS81 HAP1 lines^{28 (link)} were a gift from the Matos lab (IBC-ETH, Zurich).

Coelho M.C., Pinto R.M, & Murray A.W. (2019). Heterozygous mutations cause genetic instability in a yeast model of cancer evolution. Nature, 566(7743), 275-278.

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Tfh-Memory B Cell Coculture Protocol

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For Tfh and memory B cell coculture, at least 10 million PBMCs from HCV-infected individuals were sorted. Live CXCR3⁺ Tfh and CXCR3⁻ Tfh cells were sorted from the PBMCs as CXCR3⁺ CXCR5⁺ CD4⁺ T or CXCR3⁻ CXCR5⁺ CD4⁺ T cells, respectively, after staining with CD3 APC-Cy7, CD4 APC, CXCR5 PE-eFluor 610, and CXCR3 PE. Autologous memory B cells were sorted from the PBMCs as CD3⁻ CD19⁺ CD27⁺ B cells after staining with CD27 PE-cy7, CD3 APC-Cy7, and CD19 FITC. Sorting was conducted using a MoFlo XDP sorter (Beckman Coulter, Brea, CA, USA).

Zhang J., Liu W., Wen B., Xie T., Tang P., Hu Y., Huang L., Jin K., Zhang P., Liu Z., Niu L, & Qu X. (2019). Circulating CXCR3+ Tfh cells positively correlate with neutralizing antibody responses in HCV-infected patients. Scientific Reports, 9, 10090.

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Sorting E2-Crimson-expressing Cells

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Sorting of stably E2‐Crimson‐expressing cells was undertaken using a MoFlo XDP sorter (Beckman Coulter, Brea, CA, USA) equipped with a 676‐nm Kr laser and a 700‐nm long‐pass emission filter.

Ito D., Yogosawa S., Mimoto R., Hirooka S., Horiuchi T., Eto K., Yanaga K, & Yoshida K. (2017). Dual‐specificity tyrosine‐regulated kinase 2 is a suppressor and potential prognostic marker for liver metastasis of colorectal cancer. Cancer Science, 108(8), 1565-1573.

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