Chokeberry fruits were bought at the local market. Alginic acid sodium salt (very low viscosity) was obtained from Alfa Aesar (Kandel, Germany), while low-methoxyl pectin (Genu pectin type LM-5-CS) was from CP Kelco (Lille Skensved, Denmark). Calcium chloride, ethanol, sodium acetate, potassium chloride and ammonium acetate were from Gram-mol (Zagreb, Croatia). Sodium carbonate was purchased from T.T.T. (Sveta Nedelja, Croatia). Potassium persulfate and Folin-Ciocalteu reagent were procured from Kemika (Zagreb, Croatia). Cupric chloride, neocuproine and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were bought from Acros Organic (Geel, Belgium). methanol (HPLC grade) was from J.T. Baker (Deventer, The Netherlands) and orthophosphoric acid (HPLC grade, >85%) was from Fisher Scientific (Loughborough, UK). Hydrochloric acid (37%) and methanol were bought from Carlo Erba Reagents (Sabadell, Spain) and acetic acid (>99.5%) was from Alkaloid (Skopje, North Macedonia). Trolox, 2,2-diphenyl-1-picrylhydrazil, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 4-dimethyl-amino-cinnamaldehyd and most of the standards used for HPLC analysis (chlorogenic acid, quercetin and rutin) were from Sigma-Aldrich (St. Louis, MO, USA), while the standards of cyanidin-3-galactoside, neochlorogenic acid and hyperoside were products of Extrasynthese (Genay, France).
Neocuproine
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Neocuproine is a chemical compound used as a chelating agent in analytical chemistry. It has the molecular formula C₁₄H₁₂N₂ and is commonly used for the detection and quantification of copper ions in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (9 mentions)
Trolox, by Merck Group (8 mentions)
Potassium persulfate, by Kemika (7 mentions)
Rutin, by Merck Group (7 mentions)
2 4 6 tri 2 pyridyl s triazine tptz, by Thermo Fisher Scientific (7 mentions)
Copper 2 chloride, by Thermo Fisher Scientific (6 mentions)
Sodium carbonate, by Kemika (5 mentions)
Orthophosphoric acid hplc grade 85, by Thermo Fisher Scientific (5 mentions)
Cyanidin 3 glucoside, by Extrasynthese (5 mentions)
Orthophosphoric acid, by Thermo Fisher Scientific (4 mentions)
Folin ciocalteu reagent, by Kemika (4 mentions)
Chlorogenic acid, by Merck Group (4 mentions)
2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid diammonium salt, by Merck Group (4 mentions)
Cupric chloride, by Thermo Fisher Scientific (3 mentions)
2 2 diphenyl 1 picrylhydrazil, by Merck Group (3 mentions)
Hydrochloric acid (hcl), by Carlo Erba (3 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (3 mentions)
Caffeic, by Merck Group (3 mentions)
Chlorogenic, by Merck Group (3 mentions)
Phlorizin, by Merck Group (3 mentions)
10 protocols using neocuproine
1
Chokeberry Fruit Phytochemical Analysis
2
Antioxidant Potential of Protein Matrices
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Almond protein matrix was purchased from Raab Vitalfood GmbH (Rohrbach, Germany) and brown rice protein matrix was from Kernnel premium (Zagreb, Croatia). Quercetin, trolox and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate was obtained from Kemika (Zagreb, Croatia). Neocuproine, copper (II) chloride and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were obtained from Acros Organics (Geel, Belgium). Orthophosphoric acid (HPLC grade > 85%) was obtained from Fisher Scientific (Loughborough, UK), while methanol (HPLC grade) was purchased from J.T. Baker (Deventer, The Netherlands). Iron (III) chloride hexahydrate, ethanol, sodium acetate and ammonium acetate were purchased from Gram-mol (Zagreb, Croatia).
3
Analysis of Dairy Protein Bioactive Compounds
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Dairy proteins were obtained from Ingredia Functional (Arras, France). Methanol used for analysis was HPLC grade and it was obtained from J.T. Baker (Deventer, The Netherlands). Orthophosphoric acid was also HPLC grade (>85%) and it was purchased from Fisher Scientific (Loughborough, UK). 4-dimethyl-amino-cinnamaldehyd, trolox, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, 2,2-diphenyl-1-picrylhydrazil and standards for HPLC analysis (rutin, quercetin, chlorogenic acid, p-coumaric acid, (−)-epicatechin) were products of Sigma-Aldrich (St. Louis, MO, USA). The standard of cyanidin-3-rutinoside was from Phytolab GmbH (Vestenbergsgreuth, Germany) and the standard of neochlorogenic acid was the product of Extrasynthese (Genay, France). Folin-Ciocalteu reagent and potassium persulfate were products of Kemika (Zagreb, Croatia). Neocuproine, cupric chloride and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were products of Acros Organic (Geel, Belgium). Sodium carbonate was bought from T.T.T. (Sveta Nedelja, Croatia) and hydrochloric acid (37%) from Carlo Erba Reagents (Sabadell, Spain).
4
Antioxidant Capacity of Apple and Citrus Fibers
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Apple fibers were obtained from Biesterfeld AG (Zagreb, Croatia) and citrus fibers were obtained from Fiberstar (River Falls, WI, USA). Gallic acid, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and quercetin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), and copper (II) chloride were purchased from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was obtained from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade > 85%) was obtained from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, and ammonium acetate were purchased from Grammol (Zagreb, Croatia).
5
Antioxidant and Anti-Diabetic Potential of Pectin and Apple Fiber
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The apple fiber powder was supplied by Biesterfeld AG (Zagreb, Croatia) and both types of pectin were the product of CP Kelco (Atlanta, GA, USA). The 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), trolox, 4-(dimethylamino)-cinnamaldehyde (DMAC), chlorogenic, caffeic, p-coumaric, gallic and ellagic acids, rutin, quercetin, phloretin and phlorizin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The α-glucosidase (from Saccharomyces cerevisiae) and acarbose were obtained from Sigma-Aldrich (St. Louis, MO, USA). The 4-nitrophenyl-α-D-glucopyranoside was obtained from Alfa Aesar (Kandel, Germany) and potassium dihydrogen phosphate was from BDH Prolabo (Poole, UK). Potassium persulfate, Folin-Chiocalteu reagent and sodium carbonate were obtained from Kemika (Zagreb, Croatia). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were purchased from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, The Netherlands) and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and starch were bought from Gram-mol (Zagreb, Croatia). Cyanidin 3-glucoside was from Extrasynthese (Genay, France).
6
Comprehensive Phytochemical Analysis of Apple Fibers
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Apple fibers were obtained from Biesterfeld AG (Zagreb, Croatia). 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 4-(dimethylamino)-cinnamaldehyde (DMAC), trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), rutin, quercetin, phloretin, phlorizin, chlorogenic, caffeic, p-coumaric, gallic, ellagic acids, myrtenol and α-amylase (from the porcine pancreas) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Potassium persulfate, Folin–Ciocalteu reagent and sodium carbonate were purchased from Kemika (Zagreb, Croatia). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were bought from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and starch were bought from Gram-mol (Zagreb, Croatia). Cyanidin 3-glucoside was from Extrasynthese (Genay, France) and 3,5-dinitrosalicylic acid from Alfa Aesar (Kandel, Germany).
7
Citrus Fiber Antioxidant Potential Analysis
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Citrus fiber (76.1% of dietary fiber, while the rest are proteins—7%, sugars—7.2%, fats—0.77%, minerals—3.06%, and moisture—5.85%) was obtained from Fiberstar (River Falls, WI, USA). Potassium persulfate and sodium carbonate were purchased from Kemika (Zagreb, Croatia). Neocuproine, hesperidin, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and copper (II) chloride were bought from Acros Organics (Geel, Belgium). Trolox, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 4-(dimethylamino)-cinnamaldehyde (DMAC), quercetin, caffeic acid, p-coumaric acid, chlorogenic acid, rutin, gallic acid and ellagic acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). Cyanidin 3-glucoside was from Extrasynthese (Genay, France). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands), and orthophosphoric acid (HPLC grade > 85%) was from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate and Folin–Ciocalteu reagent were bought from Gram-mol (Zagreb, Croatia).
8
Analytical Characterization of Natural Compounds
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CMC was purchased from CP Kelco (Äänekoski, Finland). Sucrose was a product of Gram-mol (Zagreb, Croatia). Trehalose was obtained from Hayashibara doo (Nagase group, Tokyo, Japan). Hydrochloric acid was from Carlo Erba Reagents (Sabadell, Spain). Sodium carbonate was from T.T.T. (Sveta Nedelja, Croatia). Folin–Ciocalteu reagent and potassium persulfate were from Kemika (Zagreb, Croatia). Trolox, 2,2-diphenyl-1-picrylhydrazil, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, and most of the standards used (rutin, quercetin, quercetin-3-D-glucoside, chlorogenic acid) were products of Sigma-Aldrich (St. Louis, MO, USA), while the standards of cyanidin-3-glucoside, cyanidin-3-galactoside, cyanidin-3-arabinoside, neochlorogenic acid, and quercetin-3-D-galactoside were products of Extrasynthese (Genay, France). Cupric chloride, neocuproine, and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were purchased from Acros Organic (Geel, Belgium). Methanol (HPLC grade) was from J.T. Baker (Deventer, Netherlands) and orthophosphoric acid (HPLC grade, >85%) was from Fisher Scientific (Longhborough, UK).
9
Antioxidant Profiling of Lemnos Oregano
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Organic oregano (Origanum vulgare ssp. hirtum), native in the Greek island of Lemnos (39°55′ N 25°15′ E) and harvested during May of 2022, used in the experiments was kindly offered by Aegean Organics Ltd. (Lemnos, Greece). Oregano leaves were used. The reagents Folin–Ciocalteau, Trolox (6-hydroxy-2,5,7,8-tetremethychroman-2-carboxylic acid) and anhydrous sodium carbonate were purchased from SDS (Peypin, France). DPPH (1, 1-Diphenyl-2-picryl-hydrazyl), caffeic acid, luteolin, and apigenin were purchased from Sigma–Aldrich (St. Louis, MO, USA). Methanol, acetic acid HPLC water, and acetonitrile were purchased from Thermo-Fisher scientific (Nepean, ON, Canada). Neocuproine was purchased from Acros Organics (Fair Lawn, NJ, USA). Ammonium acetate, sodium chloride, sodium dihydrogen phosphate dehydrate, and copper chloride dihydrate were all purchased from Penta (CZ Ltd., Chrudim, Czech Republic). ABTS (2,20 -Azino-bis-(3-ethylbezothiazoline-6-sulphonic acid was purchased from Applichem (Darmstadt, Germany). Potassium persulfate was purchased from Chem-Lab (Zedelgem, Belgium).
10
Antioxidant Capacity of Apple Fiber Powder
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Apple fibers in powder form was obtained from Biesterfeld AG (Zagreb, Croatia). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), trolox, 4-(dimethylamino)-cinnamaldehyde (DMAC), chlorogenic, caffeic, p-coumaric, gallic and ellagic acids, rutin, quercetin, phloretin, phlorizin, and myrtenol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium persulfate, Folin-Chiocalteu reagent, and sodium carbonate were obtained from Kemika (Zagreb, Croatia). Neocuproine, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), and copper (II) chloride were purchased from Acros Organics (Geel, Belgium). Methanol (HPLC grade) was acquired from J.T. Baker (Deventer, The Netherlands), and orthophosphoric acid (HPLC grade > 85%) was obtained from Fisher Scientific (Loughborough, UK). Iron (III) chloride hexahydrate, sodium acetate, ethanol, ammonium acetate, and starch were bought from Gram-mol (Zagreb, Croatia). Cyanidin 3-glucoside was obtained from Extrasynthese (Genay, France), and 3,5-dinitrosalicylic acid was from Alfa Aesar (Kandel Germany). Sucrose was obtained from Fluka (Buchs, Switzerland). Trehalose was a donation from Hayashibara Company (Nagase group, Okayama, Japan).
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