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Quanta 450 environmental scanning electron microscope

Manufactured by Thermo Fisher Scientific

The Quanta 450 Environmental Scanning Electron Microscope (ESEM) is a high-performance electron microscope designed for a wide range of sample analysis applications. It provides high-resolution imaging capabilities and the ability to observe samples in their natural state without the need for extensive sample preparation.

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3 protocols using quanta 450 environmental scanning electron microscope

1

Comprehensive Materials Characterization

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FEI Quanta 450 Environmental Scanning Electron Microscope was utilized to examine the anatomy of materials. Transmission electron microscopy (TEM, Thermo Scientific Talos F200X G2 S/TEM) was tested to examine the crystal details of the materials. X-ray diffraction (XRD) measurements were used by a monochromatic Bruker D8 advance diffractometer. The composition and valence states were performed on a Thermo Scientific X-ray photoelectron spectroscopy. N2 sorption measurements were utilized using Micromeritics 3Flex Surface Characterization Analyzer with N2 as the adsorbent.
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2

Stereomicroscopic Examination and Imaging of Arachnid Genitalia

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All specimens were examined and measured using a LEICA M205 C stereomicroscope. The bodies, male palps, and receptacles were photographed using an Olympus C7070 digital camera. Images were combined using Helicon Focus version 6.7.1 image stacking software (http://www.heliconsoft.com). Endogynes were removed and treated in lactic acid before photographing. All measurements are given in millimeters. Leg measurements are shown as: total length (femur, patella, tibia, metatarsus, tarsus). The left palpi of males were photographed using an FEI Quanta 450 Environmental Scanning Electron Microscope. The following abbreviations are used in the text and figures:
CA cymbial apophysis;
Em embolus;
Re receptacle;
REC the ratio of embolus length (green line in Fig. 1D) and cymbium length (blue line in Fig. 1D);
SR spiral ridge of embolus.
All specimens treated here are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS), Beijing, China.
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3

Scanning Electron Microscopy of ZnO-Treated Leaves

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After 6 h of application of ZnO@MSN, the center
2 mm × 2 mm
region of the abaxial- and adaxial-dosed leaves were cut by a razor
blade and placed on SEM grids. After cutting, the leaf samples were
immediately coated with Pt before being analyzed by an FEI Quanta
450 Environmental Scanning Electron Microscope on the same day. The
analysis was done under high vacuum mode, 500–5000 × magnification,
accelerating voltage of 10, 000 kV. The detector used was an Everhart–Thornley
detector for all leaf samples except the control leaf, where a concentric
back scattered detector was used.
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