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Anti cd16 bv785

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Anti-CD16 BV785 is a flow cytometry reagent that binds to the CD16 receptor on the surface of immune cells. It can be used to identify and quantify CD16-expressing cells in biological samples.

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Lab products found in correlation

Anti cd14 pe, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Anti cd56 alexafluor700, by BioLegend (1 mentions) Anti cd3 bv711, by BioLegend (1 mentions) Anti cd4 pe cy5, by BioLegend (1 mentions) Anti cd8 bv605, by BioLegend (1 mentions) Anti cd19 percp cy5, by BioLegend (1 mentions) Anti cd45ra pe cy7, by BioLegend (1 mentions) Anti ccr7 alexa fluor 647, by BD (1 mentions) Anti cd19 apc cy7, by BioLegend (1 mentions) Live dead fixable near ir dead cell staining kit, by Thermo Fisher Scientific (1 mentions) Anti cd3 percp cy5.5 clone ucht1, by BioLegend (1 mentions) Fluorofix buffer, by BioLegend (1 mentions) Anti cd56 buv395, by BD (1 mentions)

Spelling variants of this product

CD16-BV785 (3 citations)

2 protocols using anti cd16 bv785

1

Multiparametric Flow Cytometry Analysis of Immune Cell Subsets

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The subsets of T lymphocytes14 (link),15 (link) and monocytes12 (link),13 (link) at baseline and at following timepoints during PU-H71 treatment were monitored by flow cytometry. Peripheral blood (PB) cells from healthy donor was used as a control. Cells were isolated by the Ficoll-Paque method from PB and bone marrow (BM) aspirates. Cells were labeled with anti-CD45 APC-H7, anti-CD33 BV650 (BD Biosciences, clone WM53, cat. 303430), anti-CD56 AlexaFluor700 (BioLegend, clone HCD56, cat. 318316), anti-CD3 BV711 (BioLegend, clone SK7, cat. 344838), anti-CD4 PE-Cy5 (BioLegend, clone OKT4, cat. 317412), anti-CD8 BV605 (BioLegend, clone SK1, cat. 344742), anti-CD19 PerCP/Cy5.5 (BioLegend, clone HIB19, cat. 302230), anti-CD14 PE (BD Biosciences, clone M5E2, cat. 555398), anti-CD16 BV785 (BioLegend, clone 3G8, cat. 302046), anti-CD45RA PE-Cy7 (BioLegend, clone HI100, cat. 304126) and anti-CCR7 Alexa Fluor 647 (BD Biosciences, clone 150503, cat. 560816). After washing, flow cytometry evaluation was performed on BD LSRFortessa. Data was analyzed using FlowJo software.
Sugita M., Wilkes D.C., Bareja R., Eng K.W., Nataraj S., Jimenez-Flores R.A., Yan L., De Leon J.P., Croyle J.A., Kaner J., Merugu S., Sharma S., MacDonald T.Y., Noorzad Z., Panchal P., Pancirer D., Cheng S., Xiang J.Z., Olson L., Van Besien K., Rickman D.S., Mathew S., Tam W., Rubin M.A., Beltran H., Sboner A., Hassane D.C., Chiosis G., Elemento O., Roboz G.J., Mosquera J.M, & Guzman M.L. (2021). Targeting the epichaperome as an effective precision medicine approach in a novel PML-SYK fusion acute myeloid leukemia. NPJ Precision Oncology, 5, 44.
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2

Comprehensive NK Cell Phenotyping

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PBMCs from healthy and untreated HIV-1-infected individuals were gently thawed by adding dropwise complete medium supplemented with 25 U/ml Benzonase Nuclease (Merck Milipore Novagen) followed by a 30 min incubation at 37°C and 5% CO2. After counting and washing with PBS, cells were incubated with LIVE/DEAD fixable Near-IR Dead Cell staining kit (Invitrogen) and the following antibodies at 4°C: anti-CD3-PerCP-Cy5.5 (clone UCHT1, BioLegend), anti-CD4-BV650 (clone RPA-T4, BioLegend), anti-CD8-AF700 (clone EB6B, BioLegend), anti-CD14-APC-Cy7 (clone HCD14, BioLegend), anti-CD19-APC-Cy7 (clone HIB19, BioLegend), anti-CD56-BUV395 (clone NCAM16.2, BD Optibuild), anti-CD16-BV785 (clone 3G8, BioLegend), anti-CD57-BV510 (clone QA17A04, BioLegend), anti-NKG2A-PE-Vio615 (clone REA110, Miltenyi), anti-NKG2C-BUV563 (clone 134591, BD Optibuild), anti-KIR2DL1/S1-APC (clone EB6B, Beckman Coulter), anti-KIR2DL1/S5-PE (clone 134211, R&D Systems), anti-KIR2DL2/L3/S2-BV711 (clone DX27, BD Optibuild), anti-KIR2DL3-AF488 (clone 180701, R&D Systems), anti-KIR3DL1-AF700 (clone DX9, BioLegend), anti-KIR3DL1/L2-PE-Vio770 (clone 5.133, Miltenyi) and anti-KIR2DS4-Biotin (clone JJC11.6, Miltenyi) with secondary Strepdavidin-BV421 (BioLegend). Cells were washed and then fixed with FluoroFix Buffer (BioLegend). Cells were analyzed by flow cytometry.
Vollmers S., Lobermeyer A., Niehrs A., Fittje P., Indenbirken D., Nakel J., Virdi S., Brias S., Trenkner T., Sauer G., Peine S., Behrens G.M., Lehmann C., Meurer A., Pauli R., Postel N., Roider J., Scholten S., Spinner C.D., Stephan C., Wolf E., Wyen C., Richert L., Norman P.J., Sauter J., Schmidt A.H., Hoelzemer A., Altfeld M, & Körner C. (2022). Host KIR/HLA-C Genotypes Determine HIV-Mediated Changes of the NK Cell Repertoire and Are Associated With Vpu Sequence Variations Impacting Downmodulation of HLA-C. Frontiers in Immunology, 13, 922252.
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