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2 protocols using annexin 2

1

Antibody Characterization Protocol

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We used the following primary antibodies for the proteins listed: GRP78 from Dr. Parkash S. Gill at USC (MAb159), CD44v3 from Thermo Fisher Scientific (BMS144, Waltham, MA), glutathione S-transferase (GST) tag from Santa Cruz Biotechnology, Inc. (sc-138, Dallas, TX), HA tag from Santa Cruz Biotechnology, Inc. (sc-805, Dallas, TX), FLAG M2 from Sigma-Aldrich (F1804, St. Louis, MO), Annexin II from BD Biosciences (610068, San Jose, CA), β-actin from Sigma-Aldrich (A5316, St. Louis, MO), Phospho-STAT3(Y705) from Cell Signaling Technology (9145, Danvers, MA), STAT3 from BD Biosciences (610190, San Jose, CA), Cyclin D1 from Santa Cruz Biotechnology, Inc. (sc-753, Dallas, TX), cleaved Caspase-3 from Cell Signaling Technology (9661, Danvers, MA), and cleaved PARP from Cell Signaling Technology (5625, Danvers, MA). Secondary antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX), LI-COR Biosciences (Lincoln, NE), and Enzo Life Sciences (Farmingdale, NY).
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2

Comprehensive Protein Expression Analysis

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β-actin (Sigma Aldrich mouse monoclonal anti-β-actin, A2228, 1:2000), 42 kDa. N-cadherin (BD Biosciences mouse monoclonal anti-N-cadherin, 610921, 1:2000) 120 kDa. E-cadherin (BD Biosciences mouse monoclonal anti-E-cadherin, 610181, 1:2000) 120 kDa. Vimentin (Sigma-Aldrich goat polyclonal anti-Vimentin, V4630, 1:1000) 58 kDa. S100A10(BD Biosciences mouse monoclonal anti-S100A10, 610070, 1:2000) 11 kDa. Annexin A2 (BD Biosciences mouse monoclonal anti-Annexin II, 610069, 1:2000) 36 kDa. GAPDH (Biochain mouse monoclonal anti-GAPDH, Y3322, 1:2000) 36 kDa. p-S6K (Cell signaling rabbit monoclonal anti-pS6K, 9205 S, 1:1000) 70 kDa. FOXC2 (Bethyl laboratories rabbit polyclonal anti-FOXC2, A302-383A, 1:1000) 65–70 kDa. PAI-1 (Cell signaling rabbit monoclonal anti-PAI-1 D9C4, 11907, 1:2000) 48 kDa. uPAR (Santa Cruz rabbit polyclonal anti-uPA H149, sc-10815, 1:300) 55 kDa. p-ERK (Erk1/2) (Cell signaling rabbit polyclonal anti-Erk1/2 (Thr202/Tyr204), 9101, 1:1000) 42, 44 kDa.
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