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A1si laser scanning confocal microscope

Manufactured by Nikon
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Sourced in Japan

The Nikon A1Si Laser Scanning Confocal Microscope is a high-performance imaging system that utilizes laser technology to capture detailed, high-resolution images of samples. It is designed to provide researchers with a powerful tool for exploring the structures and dynamics of biological specimens at the cellular and subcellular levels.

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Lab products found in correlation

Fluorescent in situ hybridization kit, by RiboBio (13 mentions) Fish kit, by RiboBio (11 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (4 mentions) Fluorescence in situ hybridization kit, by RiboBio (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (3 mentions) Nis elements ar, by Nikon (2 mentions) Chamber slide cultures, by Thermo Fisher Scientific (2 mentions) Vector hematoxylin qs, by Vector Laboratories (2 mentions) Bcat1, by Merck Group (2 mentions) Smad5, by Merck Group (2 mentions) Anti proteasome 20s alpha beta, by Abcam (2 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (2 mentions) α sma, by Merck Group (2 mentions) Dab substrate kit, by Vector Laboratories (2 mentions) Vectamount, by Vector Laboratories (2 mentions) Biotinylated secondary antibody, by Vector Laboratories (2 mentions) Cy3 labeled circsipa1l1 probes, by RiboBio (2 mentions) Fluorescent in situ hybridization kit, by GenePharma (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Beyotime (2 mentions)

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A1si Confocal Laser Microscope Laser scanning confocal microscope A1Si confocal microscope Laser scanning microscope Laser confocal microscope Confocal microscope

86 protocols using a1si laser scanning confocal microscope

1

Mitochondrial NADH/NAD+ Sensor Imaging

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Mitochondrial NADH/NAD+ sensor, RexMito, was prepared as previously described52 . Mia Paca-2 cells were transfected using Lipofectamine 2000 Reagent (Thermo) according to the manufacturer’s protocol. Then cell medium was replaced by complete medium, BCAA deprived medium or BCAA deprived medium with BCKA. We used a Nikon A1Si Laser Scanning Confocal Microscope to visualize the fluorescence of transfected cells 24-48 h after transfection. Fluorescence detection was carried out using 488 laser line for RexYFP and 561 laser line for HyPerRed-C199S. Imaging intensity was measured, and Ratio imaging were generated by Nikon NIS-Elements AR.
Zhu Z., Achreja A., Meurs N., Animasahun O., Owen S., Mittal A., Parikh P., Lo T.W., Franco-Barraza J., Shi J., Gunchick V., Sherman M.H., Cukierman E., Pickering A.M., Maitra A., Sahai V., Morgan M.A., Nagrath S., Lawrence T.S, & Nagrath D. (2020). Tumor-Reprogrammed Stromal BCAT1 Fuels Branched Chain Ketoacid Dependency in Stromal-Rich PDAC Tumors. Nature metabolism, 2(8), 775-792.
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2

Immunofluorescence Imaging of EWSR1 in Tumor Cells

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Tumor cells were grown on coverslips, incubated with antibodies specific for EWSR1 (ab93837; Abcam Inc.; 1:100 dilution) at 4°C overnight, and treated with Alexa Fluor 594 goat anti‐rabbit IgG (1:1,000 dilution) and DAPI (300 nM) staining. The images were photographed under a Nikon A1Si Laser Scanning Confocal Microscope and applied for 3D reconstruction using NIS‐Elements Viewer (Nikon Instruments Inc., Japan).
Li H., Yang F., Hu A., Wang X., Fang E., Chen Y., Li D., Song H., Wang J., Guo Y., Liu Y., Li H., Huang K., Zheng L, & Tong Q. (2019). Therapeutic targeting of circ‐CUX1/EWSR1/MAZ axis inhibits glycolysis and neuroblastoma progression. EMBO Molecular Medicine, 11(12), e10835.
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3

Zebrafish Pancreatic β-cell Ablation Imaging

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Fluorescence imaging of the transgenic zebrafish was conducted to identify the ablation of β-cells in the pancreas. The pancreases of zebrafish were fixed overnight in 4% paraformaldehyde. For histological analysis, the fixed samples were embedded in 1.5% agar blocks containing 5% sucrose. Frozen blocks were sectioned into 10 μm thick slices using a cryostat microtome (Leica, Wetzlar, Germany), and the sectioned slices were rinsed with phosphate-buffered saline (PBS). Sectioned slices were stained with 4′,6-diamidino-2-phenylindole (DAPI, D1306, Thermo Fisher Scientific, Waltham, MA, USA) to counterstain the cells. All fluorescence images were captured using an A1Si laser-scanning confocal microscope (Nikon, Tokyo, Japan). Confocal images (1 μm z-stacking) were processed using NIS-Elements AR Analysis 4.30 software (Nikon).
Kim J., Kim S, & Choi W.J. (2023). Non-Invasive Monitoring of Cutaneous Wound Healing in Non-Diabetic and Diabetic Model of Adult Zebrafish Using OCT Angiography. Bioengineering, 10(5), 538.
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4

Fluorescent in situ hybridization of miR-146a-5p and circGNAQ

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Cy3-labeled locked nucleic acid miR-146a-5p probe and FAM-labeled circGNAQ probe were designed and synthesized by GenePharma (Suzhou, China). The signals of the probes were detected with a Fluorescent In Situ Hybridization Kit (GenePharma, China) according to the manufacturer’s instructions. The images were acquired with a Nikon A1Si Laser Scanning Confocal Microscope (Nikon Instruments, Japan).
Wu W.P., Zhou M.Y., Liu D.L., Min X., Shao T., Xu Z.Y., Jing X., Cai M.Y., Xu S., Liang X., Mo M., Liu X, & Xiong X.D. (2021). circGNAQ, a circular RNA enriched in vascular endothelium, inhibits endothelial cell senescence and atherosclerosis progression. Molecular Therapy. Nucleic Acids, 26, 374-387.
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5

Immunohistochemistry and Immunofluorescence Analysis of Pancreatic Ductal Adenocarcinoma

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Tissues were fixed in 10% formalin overnight and embedded in paraffin. PDAC sections were deparaffinized in xylene, rehydrated through sequential ethanol, and rinsed in PBS. Non-specific signals were blocked using 10% goat serum in 0.1% Triton X-100. Tumor samples were stained with the following primary antibodies: αSMA(Sigma, A5228, 1:500), BCAT1 (Sigma, HPA048592, 1:200), SMAD5 (Sigma, HPA058931, 1:200), Ki-67(Santa Cruz Biotechnology,sc-239001:500), PCNA(Santa Cruz Biotechnology, sc-561:500). After overnight incubation, the slides were washed and incubated with biotinylated secondary antibody (Vector Laboratories) for 30min at room temperature. All slides were then incubated with avidin-biotin peroxidase complex for 30 min, and the signals were visualized by using DAB Substrate Kit (Vector Laboratories). The tissue sections were counterstained with VECTOR Hematoxylin QS and mounted with VectaMount after dehydration. IF staining was performed on tissue slices or chamber slide cultures (Thermo Fisher Scientific). The primary antibody was Anti-Proteasome 20S alpha + beta (Abcam, ab22673). Samples were mounted on microscope slides with Prolong Antifade with DAPI and imaged using a Nikon A1Si Laser Scanning Confocal Microscope.
Zhu Z., Achreja A., Meurs N., Animasahun O., Owen S., Mittal A., Parikh P., Lo T.W., Franco-Barraza J., Shi J., Gunchick V., Sherman M.H., Cukierman E., Pickering A.M., Maitra A., Sahai V., Morgan M.A., Nagrath S., Lawrence T.S, & Nagrath D. (2020). Tumor-Reprogrammed Stromal BCAT1 Fuels Branched Chain Ketoacid Dependency in Stromal-Rich PDAC Tumors. Nature metabolism, 2(8), 775-792.
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6

Visualizing circCABIN1 and miR-637 Expression

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The circCABIN1 and miR-637 FISH probes were designed and synthesized by GenePharma (Shanghai, China). To hybridize the probes to cells and tissues, the Fluorescent in Situ Hybridization Kit (RiboBio, China) was used following the manufacturer’s manual. All images were acquired on Nikon A1Si Laser Scanning confocal microscope.
Liu X., Guo Q., Gao G., Cao Z., Guan Z., Jia B., Wang W., Zhang K., Zhang W., Wang S., Li W., Hao Q., Zhang Y., Li M., Zhang W, & Gu J. (2023). Exosome-transmitted circCABIN1 promotes temozolomide resistance in glioblastoma via sustaining ErbB downstream signaling. Journal of Nanobiotechnology, 21, 45.
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7

Confocal Microscopy Multicolor Imaging

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For image acquisition, a Nikon A1si laser scanning confocal microscope was used with a Plan Fluor DIC 40x magnifying, 1.3-numerical aperture (N.A.) oil-immersion objective. NIS Elements software (version 3.21.03, Nikon, Tokyo, Japan) was employed for data analysis. Cell images were acquired in three channels for DAPI (excitation at 399 nm with laser power 10 arbitrary units [AU], emission collection at 450 nm; nuclei labeling), Alexa Fluor 488 (excitation wavelength 488 nm with laser power 10 AU and, emission wavelength at 525 nm (corresponds to a green channel, galectin-9), Alexa Fluor 555 (excitation 561 nm with laser power 10 AU, emission collection at 595 nm, red channel, Tim-3), with a photomultiplier tube gain of 100 AU. No offset was used, and pinhole size was set between 1.2 and 2 times the Airy disk size of the used objective, depending on signal strength.
Yasinska I.M., Sakhnevych S.S., Pavlova L., Teo Hansen Selnø A., Teuscher Abeleira A.M., Benlaouer O., Gonçalves Silva I., Mosimann M., Varani L., Bardelli M., Hussain R., Siligardi G., Cholewa D., Berger S.M., Gibbs B.F., Ushkaryov Y.A., Fasler-Kan E., Klenova E, & Sumbayev V.V. (2019). The Tim-3-Galectin-9 Pathway and Its Regulatory Mechanisms in Human Breast Cancer. Frontiers in Immunology, 10, 1594.
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8

Visualizing circSIPA1L1 Expression

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Cy3-labeled circSIPA1L1 probes were purchased from RiboBio. A Fluorescent in situ Hybridization Kit was employed to detect the signals of the probes following the kit instructions. The FISH sequence for circSIPA1L1 was as follows: 5′CY3-AGGGAGAAAGCATGGGATTATG 3′. The images were captured using Nikon A1Si Laser Scanning Confocal Microscope (Japan).
Xu Y., Yao T., Ni H., Zheng R., Huang K., Huang Y., Gao J., Qiao D., Shen S, & Ma J. (2021). Circular RNA circSIPA1L1 Contributes to Osteosarcoma Progression Through the miR-411-5p/RAB9A Signaling Pathway. Frontiers in Cell and Developmental Biology, 9, 642605.
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9

Phospho-FAK and F-actin Imaging in Zebrafish Embryos

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Embryos at 24 hpf were fixed in 4% paraformaldehyde (PFA) overnight at 4 degrees and then processed as previously described [78] (link), [79] (link). A mouse anti-Phospho-FAK (Tyr397) (1∶200, Invitrogen) was applied as the primary antibody, then a goat anti-mouse IgG conjugated Cy3 (Jackson ImmunoResearch Labs INC) was used as the secondary antibody at 1∶400 dilution. To visualize F-actin, phalloidin conjugated with Alexa Fluor 488 (1∶200, Life Technologies) was added with the secondary antibody. The dorsal somites were dissected from the embryo and mounted under a cover slip. Images were collected using a 20X objective on a Nikon A1-si Laser Scanning Confocal microscope.
Maragh S., Miller R.A., Bessling S.L., Wang G., Hook P.W, & McCallion A.S. (2014). Rbm24a and Rbm24b Are Required for Normal Somitogenesis. PLoS ONE, 9(8), e105460.
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10

Whole-Mount Immunohistochemistry for Axon Labeling

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For whole-mount immunohistochemistry, larvae were immersed overnight in 4% formaldehyde at 4 °C, washed three times at room temperature in phosphate-buffered saline (PBS)/0.02% Triton X-100, pH 7.3, incubated in 2% BSA with 5% sheep serum (Cat #013–000-12, Jackson Immuno, West Grove, PA, USA) for 2 h at room temperature, and then incubated overnight with primary antibodies at 4 °C. For labeling axons, mouse anti-acetylated tubulin (1:1000, Cat #T6793, Sigma) and Alexa 647-conjugated secondary antibodies (1:500, Cat #A-21235, Molecular Probes, Thermo Fisher Scientific, Waltham, MA, USA) were used.
All fluorescent images were taken in the 2-somite area above the end of the yolk extension using an A1Si laser-scanning confocal microscope (Nikon, Tokyo, Japan). Confocal images (1-μm z-stacking) were processed using NIS-Elements AR Analysis 4.30 software (Nikon).
Kim S., Lee D.W., Schachner M, & Park H.C. (2021). Small compounds mimicking the adhesion molecule L1 improve recovery in a zebrafish demyelination model. Scientific Reports, 11, 5878.
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