Mice were handled for 3 days before contextual fear conditioning, which consisted of 2 min of exploration, followed by 3 electric foot-shocks (0.5 mA, 2 s) administered 1 min apart, with an additional 1 min of exploration before removal from the chamber. Untrained control mice remained in their home-cage. Memory recall was assessed 24 h after training and consisted of re-exposure to the context for 3 min in the absence of shock. Percent of time spent freezing was taken as an index of memory and was scored by automated software (FreezeFrame, Coulbourn Instruments). For ChIP sequencing experiments, tissue was collected 30 min after training and for RNA sequencing, tissue was collected 1 h after training based on results of previous studies with H2A.Z4 (link).
Freezeframe
Manufactured by Harvard Apparatus
Sourced in United States
FreezeFrame is a laboratory equipment product that allows for the rapid freezing of biological samples. It is designed to preserve the structural integrity and chemical composition of the samples during the freezing process.
Automatically generated - may contain errors
Lab products found in correlation
Stainless steel grid shock floor, by Harvard Apparatus (3 mentions)
Freezeview, by Harvard Apparatus (3 mentions)
Researchir max 4, by Teledyne (2 mentions)
Conditioning chamber, by Harvard Apparatus (2 mentions)
Ethovision xt 11, by Noldus (1 mentions)
Operant conditioning chamber, by Harvard Apparatus (1 mentions)
Fear chamber, by Harvard Apparatus (1 mentions)
H10 11m tc, by Harvard Apparatus (1 mentions)
Video freeze, by Med Associates (1 mentions)
Test chamber, by Harvard Apparatus (1 mentions)
Electrified grid floor, by Harvard Apparatus (1 mentions)
26 protocols using freezeframe
1
Contextual Fear Conditioning in Mice
2
Contextual Fear Conditioning in Mice
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After 3-min handling for 4-consecutive days, mice were trained for contextual fear conditioning. Mice were placed in a conditioning chamber and presented with a single electrical foot shock (0.6 mA for 2 sec). Then, mice were returned to the dam. For fear memory test, mice were placed in the same chamber and exposed to the same context for 3 min at 1 h or 24 h after training. Freezing (immobile posture except for respiration) level was measured automatically using a computer program (Freeze Frame, Coulbourn Instruments).
3
Contextual and Cue Fear Conditioning Protocol
4
Learned Light Aversion in Mice
5
Thermal Stimulation and Neuronal Responses
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Behavioral and neuronal responses to a thermoneutral zone were evaluated using custom thermal chambers built by the Section for Instrumentation of the NIMH. Each chamber was equipped with a temperature sensor (Omega), a low-speed ventilator and a thermal camera (FLIR Systems) for imaging temperature gradients across the chamber (ResearchIR Max 4 software, FLIR). The ventilator itself was equipped with an in-line heater and a temperature controller (Omega) commanded by FreezeFrame (Coulbourn Instruments). Upon TTL command, the temperature ramped from room temperature to the desired value (31 °C). Mice were presented with three heating bouts lasting 1 min each. During the heating episodes animals systematically approached the heat source. Concomitant changes in GCaMP6s fluorescence in neurons of the PVT were assessed using fiber photometry.
6
Contextual and Cue Fear Conditioning Protocol
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The fear conditioning apparatus consists of a box (30 × 24 × 21 cm, Coulbourn Instruments, Whitehall, PA) with an electric grid floor (0.8 cm spacing, 4.8 mm diameter rods). Mice were placed in the box for habituation for 600 s. On the second day of the paradigm, mice were placed back in the box for 120 s followed by a 30 s tone (85 dB, 2 kHz) with a 0.7 mA foot shock delivered through the grid floor during the last 2 s of the tone. The animals remained in the box for an additional 60 s after the shock for a total duration of 210 s of training. At 24 h after training, mice were placed in the same box and freezing behavior was measured for 300 s to assess contextual fear conditioning. At 1 h after contextual fear assessment, cue fear conditioning was assessed by placing mice in a chamber with different wall coloration, lighting and scent and measuring freezing behavior for 360 s during which the tone (85 dB, 2 kHz) was delivered for the last 180 s. The box was cleaned with 70% EtOH and an enzymatic cleaner between animals. Freezing behavior was quantified using video-based analysis software (FreezeFrame, Coulbourn Instruments, Whitehall, PA).
7
Trace Fear Conditioning Protocol
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The experiment was performed from PD24 to PD26. The equipment was cleaned with 75% ethanol between trials. On the first day, each rat was acclimated in a training box for 12 min. The next day, the rat was allowed to explore the same box for 3 min and then to experience four consecutive training blocks. Each training block consisted of a 20‐s 1‐kHz 80‐dB tone (conditioned stimulus, CS), followed by an 18‐s trace interval and a 2‐s 1.0‐mA foot shock (unconditioned stimulus, US) before a 40‐s intertrial interval (ITI). On the third day, the rat's performance in the training box was recorded for 3 min to assess fear memories associated with environmental condition. Then, the rat was put into a novel chamber to test its conditioned memories of the tone (CS) associated with shock (US) in the absence of contextual cues. After adaptation for 3 min, the rat was tested with two blocks consisting of a 20‐s 1‐kHz 80‐dB tone, a 20‐s trace interval of silence and a 40‐s ITI. The experimental protocol is shown in Figure 3a . The automatic motion detection software (FreezeFrame, Coulbourn Instruments) was used to record rat behaviors during each test and calculate the percentage of freezing time, reflecting the memory of fearful events.
8
Contextual Fear Conditioning in Mice
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Mice at 4–5 months of age were placed in the conditioning chamber (Coulbourn instruments) and, after a 2-min adaptation period, received three foot shocks (2 s, 0.5 mA) at 2, 3, and 4 min. After the foot shock, mice remained in the chamber for an additional minute and then were returned to their home cage. After 24 h, mice were placed back into the same context for 5 min, and freezing was monitored. Data acquisition and analysis were measured using FreezeFrame (Coulbourn Instruments).
9
Thermal Stimulation and Neuronal Responses
10
Fear Conditioning in Rats: Tone Pre-exposure
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