B6 cg tg actflpe 9205dym j

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B6.Cg-Tg(ACTFLPe)9205Dym/J is a transgenic mouse strain that expresses the Flp recombinase enzyme under the control of the human beta-actin promoter. This strain can be used to induce site-specific recombination in cells and tissues expressing the Flp enzyme.

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10 protocols using b6 cg tg actflpe 9205dym j

Conditional Knockout Mice for CDKL5 Study

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The Cdkl5 floxed mouse line with Cre-dependent excision of exon 7 (based on revised Cdkl5 gene nomenclature; formerly exon 6) was used for the generation of conditional knockout mice^{14 (link)}. Dlx5/6-Cre, a mouse line expressing Cre in forebrain inhibitory neurons^{17 (link)}, was obtained from Jackson Laboratories and maintained in the C59BL/6J background (Stock No. 008199). The CDKL5 R59X knock-in line was generated as follows: a targeting vector was designed to insert a frt-flanked neomycin resistance (neo) cassette downstream of exon 5, and a single-nucleotide change of C to T, leading to a nonsense mutation at arginine 59 (R59X) of the Cdkl5 gene. The construct was electroporated into C57BL/6N embryonic stem (ES) cells. Correctly targeted ES cells were injected into BALB/c blastocysts and resulting chimeric mice were bred with B6.Cg-Tg(ACTFLPe)9205Dym/J (Jackson Laboratories, Stock No. 005703) to remove the neo cassette. Resulting offspring were bred to C57BL/6J mice (Jackson Laboratories, Stock No. 000664) for at least ten generations. The R59X knock-in mouse line has been deposited at Jackson Laboratories (Stock No. 028856).

Tang S., Terzic B., Wang I.T., Sarmiento N., Sizov K., Cui Y., Takano H., Marsh E.D., Zhou Z, & Coulter D.A. (2019). Altered NMDAR signaling underlies autistic-like features in mouse models of CDKL5 deficiency disorder. Nature Communications, 10, 2655.

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Conditional Knockout of Acly in Mice

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A Knockout first targeting vector was obtained from the KnockOut Mouse Project (KOMP) that targets exon 9 of Acly (ID: 80097), predicted to result in a truncated protein subject to nonsense-mediated decay. The Knockout First allele is initially null, but can be converted to a conditional floxed allele upon Flp recombination (Skarnes et al., 2011 (link)). Recombinant 129/B6 hybrid ES cells were generated in Penn’s Gene Targeting Core and blastocysts injected at Penn’s Transgenic and Chimeric Mouse Core. Upon acquisition of the chimeric mice, animals were bred to obtain germline transmission. Acly^f/+ progenies were selected through sequential breeding with wild-type C57Bl/6J mice (purchased from Jackson labs) and mice expressing Flp recombinase (B6.Cg-Tg(ACTFLPe)9205Dym/J, Jackson laboratories). Finally, Acly^f/f mice were generated by interbreeding and selected by genotyping (see Supplement). Immortalized Acly^f/f MEFs were generated from these mice (see Supplement). To produce Acly^FAT−/− mice, Acly^f/f mice were bred to Adiponectin-Cre transgenic mice (Stock No: 010803; B6;FVB-Tg(Adipoq-cre)1Evdr/J, Jackson labs). The University of Pennsylvania’s Institutional Animal Care and Use Committee (IACUC) approved all animal experiments.

Zhao S., Torres A., Henry R.A., Trefely S., Wallace M., Lee J.V., Carrer A., Sengupta A., Campbell S.L., Kuo Y.M., Frey A.J., Meurs N., Viola J.M., Blair I.A., Weljie A.M., Metallo C.M., Snyder N.W., Andrews A.J, & Wellen K.E. (2016). ATP-citrate lyase controls a glucose-to-acetate metabolic switch. Cell reports, 17(4), 1037-1052.

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Generation of PKCε Mutant Mice

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The targeting vector, PKCε mutant embryonic stem cells, and PKCε floxed mice were generated on the C57BL/6 background as described in Figure 1 at the University of Wisconsin Biotechnology Center's Transgenic Animal Facility. Homozygous floxed PKCε (PKCε^LoxP/LoxP) mice were generated by intercrossing heterozygous floxed females and males. Removal of the neomycin cassette and selection of PCK-targeted clones, was achieved by crossbreeding male chimeric mice (F1) with female FLP recombinase mice (B6.Cg-Tg (ACTFLPe)9205Dym/J) that were obtained from Jackson Laboratory (Stock # 005703). All of the animal protocols were approved by the University of Wisconsin Research Animal Resources Committee in accordance with the NIH Guideline for the Care and Use of Laboratory Animals.

Hafeez B.B., Meske L., Singh A., Singh A., Zhong W., Powers P., John M., Griep A.E, & Verma A.K. (2016). Tissue-specific conditional PKCε knockout mice: a model to precisely reveal PKCε functional role in initiation, promotion and progression of cancer. Oncotarget, 7(22), 33069-33080.

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Generation of Rora Conditional Knockout Mice

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All animal studies were conducted under approved protocols from Institutional Animal Care and Use Committee. Rora^{tm1a(EUCOMM)Wtsi} (Rora^tm1a/+) embryonic stem cells were obtained from INFRAFRONTIER/EMMA (European Mouse Mutant Archive, Munich, Germany) and subjected to in vitro injection into the C57BL/6 female mice to generate the Rora^tm1a/+ mice (performed by Animal Models of Biotechnology at the University of Wisconsin, Madison, WI, USA). A male Rora^tm1a/+ mouse was crossed with a female Sox2 (SRY-box containing gene 2)-Cre transgenic mouse (B6.Cg-Edil3^{Tg(Sox2-cre)1Amc}/J; The Jackson Laboratory, Bar Harbor, ME, USA) [69 (link)] to generate the Rora^tm1b/+ strain (Rora^LacZΔ/+), which contains a LacZ-cassette upstream the deleted Rora exon 3 (Figure 1A). To generate the Rora^tm1c/+ (Rora^flox/+) mice, the Rora^tm1a/+ mice were crossed with ACTB-FLPe transgenic mice (B6.Cg-Tg(ACTFLPe)9205Dym/J; The Jackson Laboratory) to delete the FRT-flanked section. The Rora^flox/+; FLP mice were backcrossed with wildtype C57BL/6 mice to remove FLP [39 (link)]. The Rora^LacZΔ/EKO mice were generated by crossing the Rora^flox/flox strain with the Rora^LacZΔ/+;K14-Cre strain (B6N.Cg-Tg(KRT14-cre)1Amc/J, The Jackson Laboratory). The Rora^EKO mice were generated by crossing the Rora^flox/flox strain with the K14-Cre strain.

Hua X., Blosch C.D., Dorsey H., Ficaro M.K., Wallace N.L., Hsung R.P, & Dai J. (2023). Epidermal Loss of RORα Enhances Skin Inflammation in a MC903-Induced Mouse Model of Atopic Dermatitis. International Journal of Molecular Sciences, 24(12), 10241.

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Conditional Deletion of Cap2 in Mice

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We obtained three different ES clones (C08, E05 and D07) European Conditional Mouse Consortium (EUCOMM). Two clones passed our quality control PCR tests (C08, E05) and were injected into Balb/c embryos (Charles River Laboratories, Wilmington, MA), which were then transferred to pseudopregnant CD1 females (Charles River Laboratories); chimeric offspring were identified by the presence of black hair. Chimeric males were mated to C57BL/6J females to obtain ES cell-derived offspring, which were analyzed by PCR of toe DNA to identify the CAP2 heterozygote mice. The cap2⁻ construct inserts sequences that disrupt the CAP2 gene along with FRT sites flanking the insert and loxP sites flanking exon 3. Crossing cap2⁻ mice to actin-FLP mice (B6.Cg-Tg(ACTFLPe)9205Dym/J; stock number 005703, Jackson Labs) created cap2^loxp mice, which deleted most of the insert restoring CAP2 expression but leaving the two loxP sites flanking exon 3. Crossing the cap2^loxp mice to Myh6-Cre mice (B6.FVB-Tg(Myh6Cre)2182Mds/J; stock number 011038, Jackson Labs) created cap2^loxp/cap2^loxpMyh6-Cre, which are heart specific deletions of CAP2. Figure 1a shows the three transgenic genotypes, cap2⁻/cap2⁻, cap2^loxp/ cap2^loxp and cap2^loxp/cap2^loxpMyh6-Cre.

Field J., Ye D.Z., Shinde M., Liu F., Schillinger K.J., Lu M., Wang T., Skettini M., Xiong Y., Brice A.K., Chung D.C, & Patel V.V. (2015). CAP2 in cardiac conduction, sudden cardiac death and eye development. Scientific Reports, 5, 17256.

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Generation of Conditional Sdha Knockout Mice

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C57BL/6 (WT B6, H-2K^b, CD45.2), B6 Ly5.2 (H-2K^b, CD45.1), B6.129S7-Rag1^tm1Mom/J(Rag-1^−/−), Sdhaf1^−/−, B6.Cg-Tg(ACTFLPe)9205Dym/J, B6.Cg-Tg(Vil-cre)1000Gum/J mice, C3H.SW (H-2K^b, CD229.1), and C57BL/6N-Acod1em1(IMPC)J/J (Irg1^−/−) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). BALB/c (H-2K^d) and BDF1 (H-2K^b/d) mice were purchased from Charles River Laboratories. C57BL/6N-Sdha^{tm2a(KOMP)Wtsi} mice were obtained from Knock Out Mouse Project (KOMP) repository, University of California, Davis and bred to ACTFLPe mice to excise the FRT-flanked region (Extended Data Figure 6g). Then Sdha^fl/fl mice were bred to Vil1-Cre mice to create Vil1-Cre Sdha^fl/fl (Sdha^Δ/IEC) mice. 8–12 weeks old female mice used for experiments. All mice were kept under specific pathogen-free (SPF) conditions and cared for according to regulations reviewed and approved by the University of Michigan Committee on the Use and Care of Animals (PRO00009494), which are based on the University of Michigan Laboratory Animal Medicine guidelines

Fujiwara H., Seike K., Brooks M.D., Mathew A.V., Kovalenko I., Pal A., Lee H.J., Peltier D., Kim S., Liu C., Oravecz-Wilson K., Li L., Sun Y., Byun J., Maeda Y., Wicha M.S., Saunders T., Rehemtulla A., Lyssiotis C.A., Pennathur S, & Reddy P. (2021). Mitochondrial Complex II In Intestinal Epithelial Cells Regulates T-cell Mediated Immunopathology. Nature immunology, 22(11), 1440-1451.

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Generating Liver-Specific ABCB10 Knockout Mice

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Targeted C57BL/6J ES cells with Abcb10 containing loxP sites flanking exons 2–3 and a neomycin cassette flanked by Frt sites were generated by Genoway. Targeted ES cells were injected and implanted in C57BL/6J-Tyrc-2J/J (albino) females at the Boston University Mouse Transgenic Core, directed by Gregory L. Martin and Katya Ravid. Seven male chimeras containing floxed ABCB10 were identified by coat color and bred with C57BL/6J FLP recombinase females from Jackson (B6.Cg-Tg(ACTFLPe)9205Dym/J) to excise the neomycin cassette. The offspring were bred with wild type C57BL/6J, to obtain Abcb10^wt/flox mice without FLP recombinase. Abcb10^wt/flox mice were paired with B6.Cg-Tg(Alb-cre)21Mgn/J purchased from Jackson, to generate ABCB10 liver-specific KO mice. Groups analyzed were offspring littermates from breeding pairs between Abcb10^flox/flox; Alb-Cre^−/− with Abcb10^wt/flox; Alb-Cre^+/−. Wild type mice (WT) are Abcb10^flox/flox and Abcb10^wt/flox mice (no Cre) and ABCB10-LKO are Abcb10^flox/flox; Alb-Cre^+/−.

Shum M., Shintre C.A., Althoff T., Gutierrez V., Segawa M., Saxberg A.D., Martinez M., Adamson R., Young M.R., Faust B., Gharakhanian R., Su S., Krishnan K.C., Mahdaviani K., Veliova M., Wolf D.M., Ngo J., Nocito L., Stiles L., Abramson J., Lusis A.J., Hevener A.L., Zoghbi M.E., Carpenter E.P, & Liesa M. (2021). ABCB10 exports mitochondrial biliverdin, driving metabolic maladaptation in obesity. Science translational medicine, 13(594), eabd1869.

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Generating Conditional Top1 Knockout Mice

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Knockout first ES cells targeting the Top1 gene were acquired from the KOMP Repository Knockout Mouse Project (Project ID: CSD36970, Top1^{tm1a(KOMP)Wtsi}). ES cells were microinjected into albino C57BL/6 blastocysts by the UNC Animal Models Core Facility. Two chimeric lines were bred for germline transmission. Successful germline transmitted mice were then crossed to a FLP1 recombinase deleter mouse B6.Cg-Tg(ACTFLPe)9205Dym/J (Jackson Laboratory) to excise the lacZ/neomycin cassette (removal confirmed by PCR), then backcrossed further to C57Bl/6 mice to remove the Flp transgene. To distinguish genotypes for Top1 cKO mice, the following primers flanking the LoxP site and within the Top1 gene were used: geno 2, 5’-GAGTTTCAGGACAGCCAGGA-3’ and geno 3, 5’-GGACCGGGAAAAGTCTAAGC-3’.

Mabb A.M., Simon J.M., King I.F., Lee H.M., An L.K., Philpot B.D, & Zylka M.J. (2016). Topoisomerase 1 Regulates Gene Expression in Neurons through Cleavage Complex-Dependent and -Independent Mechanisms. PLoS ONE, 11(5), e0156439.

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Generation and Characterization of Transgenic Mice

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WT/SAVI(N153S) mice were purchased from The Jackson Laboratory. HAQ, AQ mice were previously generated in the lab ^{33 (link),34 (link)}. The Q293 mice were generated by Cyagen Biosciences. Briefly, the linearized targeting vector was transfected into JM8A3.N1 C57BL/6N embryonic stem cells. A positive embryonic stem clone was subjected to the generation of chimera mice by injection using C57BL/6J blastocysts as the host. Successful germline transmission was confirmed by PCR sequencing. The heterozygous mice were bred to Actin-flpase mice [The Jackson Laboratory, B6.Cg-Tg (ACTFLPe)9205Dym/J] to remove the neo gene and make the Q293 knock-in mouse. Age- and gender-matched mice (2 – 6 month old, both male and female) were used for indicated experiments. WT/SAVI (male) x WT/HAQ (female), WT/SAVI (male) x WT/AQ (female) breeders were set up to generate HAQ/SAVI, AQ/SAVI mice. Mice were housed at 22°C under a 12‐h light‐dark cycle with ad libitum access to water and a chow diet (3.1 kcal/g, Teklad 2018, Envigo, Sommerset, NJ) and bred under pathogen-free conditions in the Animal Research Facility at the University of Florida. Littermates of the same sex were randomly assigned to experimental groups. All mouse experiments were performed by the regulations and approval of the Institutional Animal Care and Use Committee at the University of Florida, IACUC202200000058.

Aybar-Torres A., Saldarriaga L.A., Pham A.T., Emtiazjoo A.M., Sharma A.K., Bryant A.J, & Jin L. (2024). The common TMEM173 HAQ, AQ alleles rescue CD4 T cellpenia, restore T-regs, and prevent SAVI (N153S) inflammatory disease in mice. bioRxiv.

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Diverse Genetically Modified Mouse Strains

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C57BL/6J, B6.C(Cg)-Cd79a^tm1(cre)Reth/EhobJ (Cd79a^Cre) (41 (link)), B6.129S1-Irf4^tm1Rdf/J (Irf4^frt-fl) (38 (link)), B6.Cg-Tg(ACTFLPe)9205Dym/J (42 (link)), and B6.129P2(C)-Igh^tm2Cgn/J (B1-8i^+/+) (43 (link)) mice were from The Jackson Laboratory. Prdm1^fl mice (39 (link)) were a gift from K. Calame, via D. Allman. Homozygous 2F5 heavy- and light-chain knockin (2F5 KI) mice (44 (link)) were a gift from B. Haynes. Irf4^frt-fl mice were bred with flippase (FLP)-expressing deleter mice (42 (link)) to generate Irf4^+/− mice, and then Irf4^+/− and Irf4^−/− mice were obtained by interbreeding. Irf4^+/− and Irf4^−/− littermates were co-housed and continuously supplied with drinking water containing sulfamethoxazole (1 mg/ml) and trimethoprim (0.025 mg/ml) to control the otherwise high incidence of rectal prolapse in Irf4^−/− mice. All animals were on a C57BL/6 background. All experiments used age- and sex-matched, 8- to 12-week-old male or female mice. Mice were housed under specific-pathogen-free conditions at the Duke University Animal Care Facility. All experiments involving animals were approved by the Duke University Institutional Animal Care and Use Committee.

Finney J, & Kelsoe G. (2021). Continuous culture of mouse primary B lymphocytes by forced expression of Bach2. Journal of immunology (Baltimore, Md. : 1950), 207(5), 1478-1492.

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