Log In Sign up
The largest database of trusted experimental protocols

Hcv core

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in Canada

The HCV core is a laboratory instrument designed for the detection and quantification of the hepatitis C virus (HCV) core antigen. It provides a reliable and sensitive method for the diagnosis and monitoring of HCV infection.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Merck Group (2 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Ddk tag, by OriGene (1 mentions) Stat2, by Santa Cruz Biotechnology (1 mentions) Pdlim2, by Abcam (1 mentions) Nf κb p65, by Santa Cruz Biotechnology (1 mentions) Parp 1, by BD (1 mentions) β tubulin, by Abcam (1 mentions) Goat anti mouse irdye 800, by LI COR (1 mentions) Goat anti rabbit irdye 680, by LI COR (1 mentions) Alexa fluor 647 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa fluor 546 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Stat1, by Cell Signaling Technology (1 mentions) Flag tag (flag), by Fujifilm (1 mentions) Hcv ns3, by Abcam (1 mentions) P38 mapk 9212, by Cell Signaling Technology (1 mentions) Ire1α 3294, by Cell Signaling Technology (1 mentions) Perk 5683, by Cell Signaling Technology (1 mentions) Jnk 9252, by Cell Signaling Technology (1 mentions) Phospho ire1α pa1 16927, by Thermo Fisher Scientific (1 mentions)

7 protocols using hcv core

1

Antibody Characterization for Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Commercially available antibodies were used to detect FK2 (Enzo Life Sciences, BML-PW8810), DDK tag (Origene, TA50011), HCV NS3 (TORDJI-22, Abcam), HCV core (ThermoFisher Scientific, MA1-080), HAV capsid (Commonwealth Serum Laboratories, K24F2), Dengue capsid (Dr. Tom Hobman, University of Alberta), STAT1 (Cell Signaling Technologies, CST9175), STAT2 (Santa Cruz, sc-476), Y701-phosphoSTAT1 (Cell Signaling Technologies, 9167S), Y690-phosphoSTAT2 (Cell Signaling Technologies, 88410S), PDLIM2 (Abcam, 246868), p65 NF-κB (Santa Cruz, sc-372), Actin (EMD Millipore, MAB1501), PARP-1 (BD Pharmingen, BD556362), Lamin (Zymed, 33–2000), B-tubulin (Abcam, AB6046), Anti-NS5a (gift from Charlie Rice, 9E10). For western blotting, goat anti-mouse IR Dye 800 (Licor, 926–32210) and goat anti-rabbit IR Dye 680 (Licor, 926–32221) were used. For immunofluorescence, Alexa Fluor 546 goat anti-mouse IgG (ThermoFisher Scientific, A11030), Alexa Fluor 488 goat anti-rabbit IgG, (ThermoFisher Scientific, A11008), or Alexa Fluor 647 goat anti-mouse IgG (ThermoFisher Scientific, A21236) were used.
Joyce M.A., Berry-Wynne K.M., dos Santos T., Addison W.R., McFarlane N., Hobman T, & Tyrrell D.L. (2019). HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response. PLoS Pathogens, 15(8), e1007949.
+ Open protocol
+ Expand
2

Signaling Pathway Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phospho-STAT1 (Tyr701, CST, 7649), Phospho-STAT3 (Tyr705, CST, 9145, for western blot), Phospho-STAT3 (Tyr705, sc-8059, for immunoprecipitation), STAT3 (sc-482), IRF1 (CST, 8478), IRF3 (CST,11904), RELA (sc-372), MDA5 (CST, 5321), HCV NS3 (Abcam, ab13830), HCV Core (Thermo Fisher, MA1-080), HA (sigma, H9658), FLAG (WAKO, 014–22383), MERTK (sc-365499), FGFR1 (CST, 9740), IGF1Rβ (CST, 3018), β-actin (CST, 4970) and GAPDH (CST, 2118).
Xu H., Xu S.J., Xie S.J., Zhang Y., Yang J.H., Zhang W.Q., Zheng M.N., Zhou H, & Qu L.H. (2019). MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway. eLife, 8, e41159.
+ Open protocol
+ Expand
3

Antibody Optimization for Cell Stress Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Each of the primary antibodies were used according to the manufacturer’s protocol: Phospho-JNK #4671, JNK #9252, IRE1α #3294, PERK #5683, phospho-p38 MAPK #9215, p38 MAPK #9212, phospho-p44/42 MAPK #9106, p44/42 MAPK #4695, (Cell Signaling, Danvers, MA). phospho- IRE1α #PA1-16927, phospho-PERK #MA5-15033, and HCV core (Thermo Scientific, Rockford, IL). ATF6 #ab11909 (Abcam, Cambridge, MA).
Chusri P., Kumthip K., Hong J., Zhu C., Duan X., Jilg N., Fusco D.N., Brisac C., Schaefer E.A., Cai D., Peng L.F., Maneekarn N., Lin W, & Chung R.T. (2016). HCV induces transforming growth factor β1 through activation of endoplasmic reticulum stress and the unfolded protein response. Scientific Reports, 6, 22487.
+ Open protocol
+ Expand
4

Investigating Immune Receptor Signaling in HCV Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
A rabbit anti-human IFNAR1 (Abcam Inc., Cambridge, MA), a mouse anti-human IL-10Rβ (IFNλR, R&D Systems Inc., Minneapolis, MN), rabbit anti-human BiP, IRE1α, peIF2α, CHOP, Beclin 1, ATG5, LC3B, and GAPDH (Cell signaling Technology, Beverly, MA), IFNAR2, IFNγR1, CNT1 (Santa Cruz Biotechnology Inc., Santa Cruz, CA), HCV-core (Thermo Fisher Scientific, Waltham, MA), HCV-NS3 (Virogen, Watertown, MA), ENT1 (Abgent, San Diego, CA). A horseradish peroxidase-coupled antirabbit or antimouse IgG was used as secondary antibodies obtained from Cell Signaling Technology, Beverly, MA. Control siRNA (siIRR) and siRNAs against PERK and ATG7 (Life Technologies, Carlsbad, CA) were as described previously [18] (link).
Chandra P.K., Gunduz F., Hazari S., Kurt R., Panigrahi R., Poat B., Bruce D., Cohen A.J., Behorquez H.E., Carmody I., Loss G., Balart L.A., Wu T, & Dash S. (2014). Impaired Expression of Type I and Type II Interferon Receptors in HCV-Associated Chronic Liver Disease and Liver Cirrhosis. PLoS ONE, 9(9), e108616.
+ Open protocol
+ Expand
5

Western Blot Antibody Optimization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot was performed following a previously described method [35 (link)]. The dilution of primary antibodies as below: UCHL1 (diluted in 1:1000, Enzo life sciences), GSTP1 (diluted in 1:1000, Calbiochem), Vimentin and CES1 (diluted in 1:500, Santa Cruz Biotechnology), CTSB and PSME1 (diluted in 1:500, Epitomics Biotechnology). The HCV core (diluted in 1:1000, Thermo Fisher Scientific), Mouse monoclonal β-actin antibody (diluted in 1:3000, Sigma). HRP-linked anti-IgG ECL reagent (Amersham Biosciences, UK) was used as the substrate for detection, and the membrane was exposed to an X-ray film for visualization.
Ye F., Xin Z., Han W., Fan J., Yin B., Wu S., Yang W., Yuan J., Qiang B., Sun W, & Peng X. (2015). Quantitative Proteomics Analysis of the Hepatitis C Virus Replicon High-Permissive and Low-Permissive Cell Lines. PLoS ONE, 10(11), e0142082.
+ Open protocol
+ Expand
6

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell lysates were separated by SDS-PAGE. The proteins were transferred to nitrocellulose membranes and incubated overnight with primary antibodies specific to ACE2 (Abcam), HCV Core (Invitrogene), and β actin (Millipore) and then with HRP-conjugated secondary antibody for 1 h. Reactive bands were visualized with ECL reagent (Pierce) using the Platinum Q9 gel documentation system. Protein levels were quantified and normalized to β actin levels using Image Studio Lite software.
Domovitz T., Ayoub S., Werbner M., Alter J., Izhaki Tavor L., Yahalom-Ronen Y., Tikhonov E., Meirson T., Maman Y., Paran N., Israely T., Dessau M, & Gal-Tanamy M. (2022). HCV Infection Increases the Expression of ACE2 Receptor, Leading to Enhanced Entry of Both HCV and SARS-CoV-2 into Hepatocytes and a Coinfection State. Microbiology Spectrum, 10(6), e01150-22.
+ Open protocol
+ Expand
7

Quantitative Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total cell lysates were separated by SDS-PAGE. The proteins were transferred to nitrocellulose membranes and incubated overnight with primary antibodies specific to ACE2 (Abcam), HCV Core (Invitrogene), and β actin (Millipore) and then with HRP-conjugated secondary antibody for 1 h. Reactive bands were visualized with ECL reagent (Pierce) using the Platinum Q9 gel documentation system. Protein levels were quantified and normalized to β actin levels using Image Studio Lite software.
Domovitz T., Ayoub S., Werbner M., Alter J., Izhaki Tavor L., Yahalom-Ronen Y., Tikhonov E., Meirson T., Maman Y., Paran N., Israely T., Dessau M, & Gal-Tanamy M. (2022). HCV Infection Increases the Expression of ACE2 Receptor, Leading to Enhanced Entry of Both HCV and SARS-CoV-2 into Hepatocytes and a Coinfection State. Microbiology Spectrum, 10(6), e01150-22.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!