The largest database of trusted experimental protocols

2 protocols using cd86 vioblue

1

Ovalbumin-Loaded Silica Nanoparticles for Immunotherapy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rhodamine B isothiocyanate
(RITC), (3-aminopropyl)trimethoxysilane (APTMS), cethyltrimethylammonium
bromide (CTAB), ammonium hydroxide, tetraethylorthosilicate (TEOS),
and ovalbumin (OVA) were purchased from Sigma-Aldrich (St. Louis,
MO, USA). HCl, methanol, and ethyl acetate were purchased from SamChun
Chemical (Seoul, Korea). Bovine serum albumin (BSA) was purchased
from Millipore (Billerica, MA, USA), CpG oligodeoxynucleotide was
purchased from Bioneer (Daejeon, Korea). Recombinant murine GM-CSF
was purchased from Peprotech (Rocky Hill, NJ, USA). Antibodies against
the following proteins were used: CD11c-APC, MHC class-II-FITC, CD86-Vioblue,
MHC class-I(H-2Kb)/SIINFEKL-PE-Vio770, CD3e-FITC, CD4-PE-Vio770,
CD8-APC, IFN-γ-FITC, CD44-Vioblue, and CD62L-PE were purchased
from Miltenyi Biotec (Bergisch Gladbach, Germany) and H-2Kb/SIINFEKL tetramer-PE was purchased from MBL life science (Woburn,
MA). IL-12 and TNF-α ELISA kits were purchased from BD science.
+ Open protocol
+ Expand
2

Phenotypic analysis of immune cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flow cytometry was performed on MACS Quant flow cytometer (Miltenyi). For extracellular staining cells were stained with HLA-DR FITC, CD80 APC, CD86 Vio-blue (all Miltenyi), CD40 FITC, CD83 PE (BD Biosciences) for 30 min on ice. For CD68, cells were fixed and permeabilised using Inside Stain Kit (Miltenyi) and stained with CD68 FITC (Miltenyi). Flow cytometry experiments were analyzed in FlowJo software.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!