Secondary conjugated anti mouse horseradish peroxidase hrp

The following antibodies were used in western blot and immunofluorescence studies: p-AMPK, AMPK p-CREB (Cell Signaling), anti-Nrf-2, anti-PSD-95, anti-Syntaxin, anti-synaptosomal-associated protein 23 (SNAP-23), anti-Caspase 3, anti- PARP-1, anti-Cleaved Caspase-3, Synaptophysin, anti-Bax, anti-Bcl2, anti-TNF-α, anti-IL-1β, anti-p-NF-κB, anti-Iba-1, anti-GFAP, and anti-β-actin, which were all obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibodies were diluted in 1× TBST (Tris-buffered saline plus Tween) (1:1000), and secondary conjugated anti-mouse horseradish peroxidase (HRP) and conjugated anti-rabbit HRP were diluted 1:10,000 in 1× TBST, all purchased from Promega USA. For confocal microscopic studies, the secondary fluorescent antibodies used were goat anti-mouse and goat anti-rabbit diluted in 1 × 100 phosphate-buffered saline (PBS).

The following antibodies were used in immunoblotting and immunofluorescence studies: anti-Iba-1, anti-GFAP, Nrf2, HO-1, anti-TNF-α, anti-IL-1β, anti-p-NF-κB, Cox2, anti-caspase-3, anti-cytochrome-C, anti-Bax, anti-Bcl2, and anti-β-actin. These antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibodies were diluted in 1× TBST (Tris-buffered saline plus Tween) (1:1000), while secondary conjugated anti-mouse horseradish peroxidase (HRP) and conjugated anti-rabbit HRP were diluted 1:10,000 in 1× TBST, all these were purchased from Promega, Madison, WI, USA. For immunofluorescence studies, the secondary fluorescent antibodies goat anti-mouse and goat anti-rabbit were used, and diluted in 1 × 100 phosphate-buffered saline (PBS).