Pooled human plasma

Manufactured by Innovative Research

Sourced in United States

Pooled human plasma is a biological material consisting of the liquid portion of human blood, obtained from multiple donor sources. It serves as a source of various blood components, including proteins, clotting factors, and other biomolecules.

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Lab products found in correlation

Pooled human saliva, by Innovative Research (2 mentions) Steriflip filtration units, by Merck Group (2 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Iga from human serum, by Merck Group (1 mentions) Uplc ms, by Waters Corporation (1 mentions) Gibco rpmi 1640, by Thermo Fisher Scientific (1 mentions) Gibson assembly master mix, by New England Biolabs (1 mentions) Bacto tryptic soy broth, by BD (1 mentions) Bacto todd hewitt broth, by BD (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti rabbit antibody, by LI COR (1 mentions) Power sybr green master mix, by Thermo Fisher Scientific (1 mentions) Oligonucleotide, by Integrated DNA Technologies (1 mentions) Sprague dawley rat plasma, by Innovative Research (1 mentions) Losartan, by Merck Group (1 mentions) Nifedipine, by Merck Group (1 mentions) Phenacetin, by Merck Group (1 mentions) Tolbutamide, by Merck Group (1 mentions) Dextromethorphan, by Merck Group (1 mentions) Erlotinib, by Merck Group (1 mentions)

Spelling variants of this product

Human plasma (14 citations) Pooled normal human plasma (8 citations) Pooled Human Plasma Apheresis Derived (2 citations) Pooled human blood plasma (2 citations)

9 protocols using pooled human plasma

Quantification of Hydroxylated PCBs in Plasma

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All solvents were purchased from Fisher Scientific (Pittsburg, PA) and were of Optima LC–MS quality or better. Hydroxylated polychlorinated biphenyl standards were purchased from Wellington Laboratories (ON, Canada) and Accustandard, Inc. (New Haven, CT, USA). Pooled human plasma was purchased from Innovative Research (Novi, MI, USA). Nine OH-PCBs were used in this study: 2,3,3′,4′,5-Pentachloro-4-biphenylol (4-OH CB 107), 2′,3,3′,4′,5-Pentachloro-4-biphenylol (4-OH CB 108), and 2,3′,4,4′,5-Pentachloro-3-biphenylol (3-OH CB 118), 2,2′,3,3′,4′,5-Hexachloro-4-biphenylol (4-OH CB 130), 2,2′,3′,4,4′,5-Hexachloro-3-biphenylol (3-OH CB 138), and 2,2′,3,4′,5,5′-Hexachloro-4-biphenylol (4-OH CB 146), 2,2′,3,3′,4′,5,5′-Heptachloro-4-biphenylol (4-OH-PCB 172), 2,2′,3′,4,4′,5,5′-Heptachloro-3-biphenylol (3-OH CB 180), and 2,2′,3,4′,5,5′,6-Heptachloro-4-biphenylol (4-OH CB 187). Binary isomer mixtures were created by mixing equal volumes of the individual standards.

Adams K.J., Smith N.F., Ramirez C.E, & Fernandez-Lima F. (2017). Discovery and targeted monitoring of polychlorinated biphenyl metabolites in blood plasma using LC-TIMS-TOF MS. International journal of mass spectrometry, 427, 133-140.

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Pooled Human Plasma and Saliva Preparation

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Pooled human plasma (catalog number IPLA-N) and pooled human saliva (catalog number IR100044P) from healthy donors were purchased from Innovative Research, USA. Saliva was centrifuged at 1500 × g 15 min 4 °C, sterile filtered using 0.22 μm Steriflip filtration units (Millipore), concentrated to 5 mg ml⁻¹ using Millipore Amicon 3.5 kDa molecular weight cut-off concentrators, and supplemented with Protease Inhibitor Cocktail (Sigma; 10 μl ml⁻¹ saliva) prior to use. Pooled human IVIG (Octagam 100 mg ml⁻¹, catalog number 158007) were obtained from Octapharma.

Happonen L., Hauri S., Svensson Birkedal G., Karlsson C., de Neergaard T., Khakzad H., Nordenfelt P., Wikström M., Wisniewska M., Björck L., Malmström L, & Malmström J. (2019). A quantitative Streptococcus pyogenes–human protein–protein interaction map reveals localization of opsonizing antibodies. Nature Communications, 10, 2727.

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Preparation of Biological Reagents

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Pooled human plasma (lot numbers 18944 and 27744) and pooled human saliva (catalog number IR100044P) were purchased from Innovative Research, USA. Pooled saliva was centrifuged at 1,500 × g for 15 min at 4°C followed by sterile filtration using 0.22-μm Steriflip filtration units (Millipore) and storage at −20°C until further use. IgA from human serum (lot number 0000085362) was purchased from Sigma-Aldrich, Germany. Purified human complement C4BP (catalog number A109, lot number 4a) was obtained from Complement Technology, USA. The recombinant human IgA Fc domain (catalog number PR00105) was purchased from Absolute Antibody, UK.

Chowdhury S., Khakzad H., Bergdahl G.E., Lood R., Ekstrom S., Linke D., Malmström L., Happonen L, & Malmström J. (2021). Streptococcus pyogenes Forms Serotype- and Local Environment-Dependent Interspecies Protein Complexes. mSystems, 6(5), e00271-21.

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Plasma Stability Assessment of Compounds

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Compound stocks were prepared at a concentration of 10 mM in DMSO. The internal standard was 4 μg/mL warfarin in acetonitrile. Compound stocks were mixed with mouse plasma (Fisher Scientific) or pooled human plasma (Innovative Research Inc) to prepare a final concentration of 10 μM in a 2 mL 96- well deep well plate (pION Inc., Woburn, MA, catalog #110023); this was the master plate. From the master plate, 70 μL was taken from each well and added into four storage plates (pION Inc., Woburn, MA, catalog #110323) in triplicate, each for a different time point. The storage plates were then incubated at 37 °C and shaken at 100 rpm. Samples were taken at 0 min, 3h, 24 h, and 48h. At each time point, 210 μL of internal standard was added to quench the reaction. The plates were then centrifuged at 4000 rpm for 20 min, and supernatant was analyzed by Ultra High Pressure Liquid Chromatography with Mass Spectrometry (UPLC-MS; Waters Inc., Milford, MA). The compound was detected by selected ion recording (SIR), and quantitation was based on the peak area ratio of the test compound vs. the internal standard.

Griffith E.C., Zhao Y., Singh A.P., Conlon B.P., Tangallapally R., Shadrick W.R., Liu J., Wallace M.J., Yang L., Elmore J.M., Li Y., Zheng Z., Miller D.J., Cheramie M.N., Lee R.B., LaFleur M.D., Lewis K, & Lee R.E. (2019). Ureadepsipeptides as ClpP Activators. ACS infectious diseases, 5(11), 1915-1925.

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Bacterial Culture Media and Reagents

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Bacto Todd-Hewitt broth (TH) and Bacto tryptic soy broth (TSB) were purchased from Becton, Dickinson (Sparks, MD). Gibco RPMI 1640 and 0.25% trypsin-EDTA were purchased from Life Technologies Corporation (Grand Island, NY). Pooled human plasma was purchased from Innovative Research, Inc. (Novi, MI). Power SYBR green master mix was purchased from Applied Biosystems and used according to the manufacturer’s instructions (Foster City, CA). Mitomycin C (no. M4287), lysostaphin (no. L7386), and mutanolysin (no. M9901) were purchased from Sigma-Aldrich (St. Louis, MO). Goat anti-rabbit antibody–IRDye 680LT and goat anti-rabbit antibody–IRDye 800CW were purchased from LI-COR Biosciences (Lincoln, NE). Anti-GFP antibody was a kind gift of David Weiss, University of Iowa. Anti-β-toxin antibody was produced in-house. All other enzymes, polymerases, and Gibson Assembly master mix were purchased from New England Biolabs (Beverly, MA) and used according to the manufacturer’s instructions unless otherwise noted. All oligonucleotides were purchased from Integrated DNA Technologies (IDT; Coralville, IA).

Tran P.M., Feiss M., Kinney K.J, & Salgado-Pabón W. (2019). ϕSa3mw Prophage as a Molecular Regulatory Switch of Staphylococcus aureus β-Toxin Production. Journal of Bacteriology, 201(14), e00766-18.

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Stability of Paclitaxel Prodrug Formulations

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In vitro stability of o(LA)₈-PTX prodrug and o(LA)₈-PTX-PM (10 or 50%) in plasma were determined using heparinized Sprague-Dawley rat plasma (Innovative Research Inc., Novi, MI) and pooled human plasma (Innovative Research Inc., Novi, MI). Frozen plasma samples were incubated at 37 °C for 5 min before use. Stock solution of o(LA)₈-PTX was prepared in DMSO at 1 mM. In 1.0 mL plasma samples, 10 μL of stock solution was added to reach a concentration of o(LA)₈-PTX at 10 μM (< 1% DMSO in the mixture). Alternatively, 100 μL of o(LA)₈-PTX-PM (10%) or o(LA)₈-PTX-PM (50%) in PBS solution (10 mM, pH 7.4) was added to plasma (900 μL) to reach a final concentration of 10 μM. Samples were incubated at 37 °C in a temperature adjusted water bath (GCA Corporation, IL). At predetermined time intervals (0, 0.25, 0.5, 1, 2, 4, 6, 9, and 24 h), 50 μL of plasma samples were withdrawn and diluted with 100 μL of acetonitrile containing 0.1% formic acid. Precipitated samples were centrifuged at 13,000 rpm for 10 min and the supernatant containing solubilized o(LA)₈-PTX and its metabolites (o(LA)_0–8-PTX) were analyzed by RP-HPLC. The relative amount of o(LA)₈-PTX and its metabolites (o(LA)_0–8-PTX) was calculated as a percentage relative to the starting level of o(LA)₈-PTX.

Tam Y.T., Shin D.H., Chen K.E, & Kwon G.S. (2019). Poly(ethylene glycol)-block-poly(D,L-lactic acid) Micelles containing Oligo(lactic acid)8-Paclitaxel Prodrug: In Vivo Conversion and Antitumor Efficacy. Journal of controlled release : official journal of the Controlled Release Society, 298, 186-193.

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Hepatocyte Cryopreservation and CYP Assay

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The mixed-gender 200 donor pooled cryopreserved primary human hepatocytes with 5 million cells, hepatocyte thawing buffer, and incubation buffer were purchased from In Vitro Technologies (Baltimore, MD). Gefitinib, erlotinib, CYP-specific substrates (phenacetin, S-mephenytoin, tolbutamide, dextromethorphan, nifedipine), and CYP inhibitors (fluoxetine and losartan) were purchased from Sigma-Aldrich (St. Louis, MO). Recombinant human pooled supersomes for CYP1A2, CYP2C19, CYP2C9, CYP2D6, and CYP3A4, pooled human microsomes, and the NADPH regeneration system solutions A and B were obtained from Corning Life and Sciences (Tewksbury, MA). Pooled human plasma was purchased from Innovative Research (Novi, MI). RED base plate, RED device inserts, and RED dialysis buffer were purchased from Thermo Scientific.

Luong T.L., McAnulty M.J., Evers D.L., Reinhardt B.J, & Weina P.J. (2021). Pre-clinical drug-drug interaction (DDI) of gefitinib or erlotinib with Cytochrome P450 (CYP) inhibiting drugs, fluoxetine and/or losartan. Current Research in Toxicology, 2, 217-224.

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HIV-1 Immunoassay Reagents and Protocols

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The following matrixes were obtained from Innovative Research: Pooled Human Plasma (blood derived) with the anticoagulants K2 EDTA (cat# IPLAWBK2E), K3 EDTA (cat# IPLAWBK3E), Na EDTA (cat# IPLAWBNAE), Li Heparin (cat# IPLAWBLIH5), Na Heparin (cat# IPLAWBNAH), Na Citrate (cat# IPLAWBNAC), Pooled Human Cerebrospinal Fluid (cat# IRHUCSF), Pooled Human Pasteurized Breast Milk (cat# IRHUBMKPST) (pooled with a minimum of three donors), and Pooled Human AB Serum Plasma Derived (cat# ISERAB) (pooled from male donors blood type AB). Anti-HIV type 1 p24 clone 39/5.4A (cat# 0801136) was purchased from Zeptometrix. Monoclonal anti-HIV-1/2 purified antibody (cat# HIV-018-48303) was purchased from Capricorn Products LLC. The following reagents were obtained from Thermo Fisher Scientific: Sulfo-SMCC (cat# A39268), NHS-PEG₄-Biotin (cat# A39259) and Ultrapure 1 M Tris–HCl (cat# 15-567-027). Simoa Planar Array Homebrew Tag 1, Sample Diluent A, Streptavidin-HRP, Stable Peroxide, SuperSignal Luminol, Homebrew plate, and 25X wash buffer were all obtained from Quanterix. The following reagent was obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH: Human Immunodeficiency Virus 1 (HIV-1), Strain JR-CSF Infectious Molecular Clone (pYK-JRCSF), ARP-2708, contributed by Dr. Irvin SY Chen and Dr. Yoshio Koyanagi.

Levinger C., Howard J.N., Cheng J., Tang P., Joshi A., Catalfamo M, & Bosque A. (2021). An ultrasensitive planar array p24 Gag ELISA to detect HIV-1 in diverse biological matrixes. Scientific Reports, 11, 23682.

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Metabolite Extraction from Pooled Plasma

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Pooled human plasma was obtained from Innovative Research (Novi, MI). The frozen sample was thawed for 1 h on ice, vortexed briefly and separated into aliquots of 100 μL. Metabolite extraction was initiated by addition of 400 μL of methanol/acetone/acetonitrile (1:1:1 v/v/v). After 1 min of vortexing, the samples were incubated at 4 °C for 30 min. The samples were centrifuged at 14,000 rpm at 4 °C and the supernatant including the lipid layer was collected, transferred to microtubes and dried under N₂. The metabolites were reconstituted in 50 μL ACN/H2O (80:20 v/v), vortexed briefly and transferred to autosampler vials. Spiked plasma extracts for the estimation of detection limits were prepared by adding 1, 2 or 5 μL of standard mixtures containing known concentrations (millimolar range) of metabolites to plasma extract before drying. 60 metabolites were selected based on their known or expected presence in blood according to data from the human metabolome database (21 (link)) (http://hmdb.ca, accessed November 2015) and each mixture contained 10 different, non-isobaric compounds.

Wernisch S, & Pennathur S. (2016). Evaluation of coverage, retention patterns and selectivity of seven liquid chromatographic methods for metabolomics. Analytical and bioanalytical chemistry, 408(22), 6079-6091.

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