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Edta coated tube

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EDTA-coated tubes are laboratory equipment designed to collect and store blood samples. The tubes are coated with the anticoagulant EDTA, which helps prevent the blood from clotting during collection and storage.

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Lab products found in correlation

Ficoll paque, by Miltenyi Biotec (3 mentions) Ultra sensitive mouse insulin elisa kit, by Crystal Chem (2 mentions) Radioimmunoassay kit, by MP Biomedicals (2 mentions) Lineage cell depletion kit, by Miltenyi Biotec (2 mentions) Xt 2000i vet analyzer, by Sysmex (2 mentions) Xt 2000, by Sysmex (1 mentions) Mouse cd8a t cell isolation kit, by Miltenyi Biotec (1 mentions) Advia 2120i, by Siemens (1 mentions) Albino bl6 mice, by Charles River Laboratories (1 mentions) Balb c mice, by Charles River Laboratories (1 mentions) Biocoll separating solution, by Harvard Bioscience (1 mentions) Lymphocyte separation media, by Capricorn (1 mentions) Dimethyl sulfoxide (dmso), by AppliChem (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Ack lysing buffer, by Lonza (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Rpmi 1640 medium, by Biochrome (1 mentions) Amplex red reagent, by Thermo Fisher Scientific (1 mentions) Pharm lyse, by BD (1 mentions) Facscanto 2, by BD (1 mentions)

Spelling variants of this product

EDTA tubes (108 citations) EDTA-coated microvette tubes (56 citations) EDTA-coated blood collection tubes (7 citations) EDTA- and silicone-coated tubes (4 citations) EDTA-coated microcentrifuge tubes (4 citations) EDTA-coated vacutainer tubes (3 citations) EDTA-coated capillary tubes (3 citations) S-Monovette EDTA-coated tubes (2 citations) EDTA-coated microcuvette tubes (2 citations) 1.5 mL EDTA-coated microcentrifuge tubes (2 citations)

93 protocols using edta coated tube

1

Blood Collection and Analysis in Mice

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Peripheral blood was collected from the facial vein of mice into an EDTA-coated tube (Sarstedt, Nümbrecht, Germany). Blood counts were determined using an automated blood cell counter (Sysmex XT-2000, Barcelona, Spain).
Martínez-Valiente C., Garcia-Ruiz C., Rosón B., Liquori A., González-Romero E., Fernández-González R., Gómez-Redondo I., Cervera J., Gutiérrez-Adán A, & Sanjuan-Pla A. (2021). Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes. International Journal of Molecular Sciences, 22(13), 6963.
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2

Plasma CORT Profiling via Tail Nicking

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To investigate plasma CORT profiles, blood samples were collected via lateral tail nick at the time points indicated below for experiments 1A and 1B (see also Fig. 1A). An experimenter lightly restrained each rat under a blue pad near the edge of a flat surface, allowing their tail to hang off. A small (≤5 mm) nick was made with the tip of a razor blade, and blood was extracted via capillary action (∼200 μl) into an EDTA-coated tube (Sarstedt). Samples were capped, inverted two to three times, and immediately placed onto ice where they remained (<3 h) until the last tail nick was performed. Samples were then separated by centrifugation (13,000 rpm for 10 min at 4°C), and plasma was extracted, flash-frozen on dry ice, and stored at −20°C until processed for radioimmunoassay (RIA).
Lopez S.A., Mubarak E., Yang C., Parsegian A., Klumpner M., Campus P, & Flagel S.B. (2021). Male Goal-Tracker and Sign-Tracker Rats Do Not Differ in Neuroendocrine or Behavioral Measures of Stress Reactivity. eNeuro, 8(3), ENEURO.0384-20.2021.
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3

Adoptive CD8+ T Cell Transfer in FV Infection

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From donor mice, approximately 100 μL of blood was collected in an EDTA-coated tube (Sarstedt). Erythrocytes were lysed in 5 mL of 0.16 M ammonium carbonate (pH 7.2) on ice with occasional shaking for 5 minutes, followed by washing with 35 mL PBS. CD8+ T cells were isolated by magnetic activated cell sorting using a mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec) following the manufacturer’s instructions. One thousand purified cells were transferred intravenously suspended in 100 μL PBS into recipient mice, which had been infected with FV 4 hours prior to the cell transfer.
Mittermüller D., Otto L., Long Z., Kraus A., Beer A., Hasenberg A., Zelinskyy G., Westmeier J., Hasenkrug K.J., Dittmer U, & Gunzer M. (2023). Regulatory T cells suppress the motility of cytotoxic T cells in Friend retrovirus–infected mice. JCI Insight, 8(13), e167482.
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4

Pharmacokinetic Analysis of Blood and Prefrontal Cortex

Cited 1 time
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The experiment was performed using the methods described by Bridges et al. [34 (link)] and in our previous studies [21 (link),22 (link),24 (link)].
The pharmacokinetic analysis was performed in the blood and prefrontal cortex. Blood was collected from the inferior vena cava, transferred to an EDTA-coated tube (Sarstedt) and centrifuged at 3500× g rpm for 10 min at RT. Plasma and FC samples were stored at −80 °C until analysis.
Cieślik P., Radulska A., Burnat G., Kalinowski L, & Wierońska J.M. (2021). Serotonergic–Muscarinic Interaction within the Prefrontal Cortex as a Novel Target to Reverse Schizophrenia-Related Cognitive Symptoms. International Journal of Molecular Sciences, 22(16), 8612.
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5

Blood Sampling and Hematology Analysis

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Blood samples were drawn from the tail vein and collected in EDTA-coated tube (Sarstedt). 80 μL of blood were diluted in 240 μL of saline + EDTA before being analyzed on ADVIA 2120i (Siemens).
Usart M., Hansen N., Stetka J., Almeida Fonseca T., Guy A., Kimmerlin Q., Rai S., Hao-Shen H., Roux J., Dirnhofer S, & Skoda R.C. (2024). The glutaminase inhibitor CB-839 targets metabolic dependencies of JAK2-mutant hematopoiesis in MPN. Blood Advances, 8(9), 2312-2325.
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6

Murine Tumor Models for Evaluating Chemotherapeutic Agents

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All animal experiments were performed in accordance with protocols approved by the Duke Institutional Animal Care and Use Committee (IACUC). BALB/c mice (Charles River, Female, 6–10 weeks old) were inoculated with 8 × 105 4T1-luciferase cells in the 4th mammary fat pad. Albino BL6 mice (Charles River, Female, 6–10 weeks old) were shaved and inoculated subcutaneously on the flank with 1 × 106 LLC-luc cells. For all inoculations, cells were suspended in serum-free DMEM at a concentration appropriate for a 50 μL injection. CP-Dox was synthesized as described in the supplementary information. Mice were treated on day 8 (post-inoculation) with free Dox or CP-Dox at the maximum tolerated dose (5 mg/kg and 20 mg/kg, respectively). In metastasis prevention studies, tumors were surgically resected on day 15. Mice were sacrificed if they appeared moribund or lost > 15% of their baseline body weight, or if the tumor volumes exceeded 2000 mm3. Tumor volumes were calculated using the formula Volume (mm3) = length * width2/2. Mice were randomized to treatment groups using the list randomizer from random.org. For CBC analysis, 100 μL of blood was drawn from mice into an EDTA coated tube (Sarstedt, Newton, NC). Samples were run on an Idexx Procyte (Idexx Operation, Inc., Memphis, TN).
Mastria E.M., Cai L.Y., Kan M.J., Li X., Schaal J.L., Fiering S., Gunn M.D., Dewhirst M.W., Nair S.K, & Chilkoti A. (2017). Nanoparticle formulation improves doxorubicin efficacy by enhancing host antitumor immunity. Journal of controlled release : official journal of the Controlled Release Society, 269, 364-373.
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7

Corticosterone and Hormone Measurement in Mice

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Mice were euthanized by decapitation following brief CO2 exposure. Mice were sacrificed within 3 min after an experimenter entered the animal room. Trunk blood was collected in EDTA coated tubes (Sarstedt) and spun at 1,500 × g for 10 min at 4 °C for plasma separation. Corticosterone was measured by RIA as previously described [33] . Other hormones were analyzed using Bio-Plex ProTMmouse assay kits and measured with Bio-Plex1 system (Bio-Rad Laboratories, Inc., USA) following manufacturer's protocol. Obtained fluorescent intensities for each hormone were analyzed using Masterplex software.
Razzoli M., Frontini A., Gurney A., Mondini E., Cubuk C., Katz L.S., Cero C., Bolan P.J., Dopazo J., Vidal-Puig A., Cinti S, & Bartolomucci A. (2015). Stress-induced activation of brown adipose tissue prevents obesity in conditions of low adaptive thermogenesis. Molecular Metabolism, 5(1), 19-33.
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8

Plasma Corticosterone Quantification by ELISA

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Quantification of plasma corticosterone was performed using enzyme-linked immunosorbent assay (ELISA). As described before, trunk blood of FIT animals was collected after decapitation. Approximately 1 ml blood was collected in EDTA-coated tubes on ice (Sarstedt, Numbrecht, Germany), centrifuged at 4 °C (2000 × g, 10 min), aliquoted and stored at −20 °C until the assay was performed using a commercially available ELISA kit for corticosterone (IBL International, Hamburg, Germany) following the manufacturer’s protocol.
Oliveira V.E., Lukas M., Wolf H.N., Durante E., Lorenz A., Mayer A.L., Bludau A., Bosch O.J., Grinevich V., Egger V., de Jong T.R, & Neumann I.D. (2021). Oxytocin and vasopressin within the ventral and dorsal lateral septum modulate aggression in female rats. Nature Communications, 12, 2900.
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9

PBMC Isolation and Cryopreservation from Blood Samples

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Blood was collected in EDTA coated tubes (Sarstedt) and processing of blood samples was started immediately upon receipt of blood samples in the laboratory. For MS patients, NND patients and healthy individuals PBMCs were isolated by a 30 min gradient centrifugation at 860 ×g using Biocoll Separating Solution (Biochrom), followed by two washing steps. Cells were then cryopreserved in RPMI medium (PAN-Biotech) with 25% heat-inactivated fetal calf serum (FCS, Biochrom) and 10% (v/v) dimethyl sulfoxide (DMSO, AppliChem) and stored in liquid nitrogen until further analysis. For trans men PBMCs were isolated by a 30 min gradient centrifugation at 500 ×g using Lymphocyte Separation Media (Capricorn), followed by two washing steps. After lysis of erythrocytes with ACK Lysing Buffer (Lonza), cells were then cryopreserved in heat-inactivated fetal bovine serum (FBS) supplemented with 10% (v/v) dimethyl sulfoxide (SigmaAldrich) and stored in liquid nitrogen until further analysis.
Winschel I., Willing A., Engler J.B., Walkenhorst M., Meurs N., Binkle-Ladisch L., Woo M.S., Pfeffer L.K., Sonner J.K., Borgmeyer U., Hagen S.H., Grünhagel B., Claussen J.M., Altfeld M, & Friese M.A. (2024). Sex- and species-specific contribution of CD99 to T cell costimulation during multiple sclerosis. Biology of Sex Differences, 15, 41.
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10

C57BL/6J Mouse Blood Collection

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This study was performed according to animal protection guidelines issued by the Novartis Animal Welfare Policy. C57BL/6J mice (8-to 10-week-old females and males) were purchased from The Jackson Laboratory (JAX, Bar Harbor, ME, USA). Animals were housed in groups of two to five under temperature-and humidity-controlled conditions in individually ventilated cages with a 12-hour light/12-hour dark cycle. Mouse blood was collected into ethylenediaminetetraacetic acid (EDTA)-coated tubes (Sarstedt, Nümbrecht, Germany). Mouse plasma was prepared by centrifugation at 775 × g for 10 min at 4°C.
Da Cunha A.P., Zhang Q., Prentiss M., Wu X.Q., Kainz V., Xu Y.Y., Vrouvlianis J., Li H., Rangaswamy N., Leehy B., McGee T.L., Bell C.L., Bigelow C.E., Kansara V., Medley Q., Huang Q, & Wu H.Y. (2018). The Hierarchy of Proinflammatory Cytokines in Ocular Inflammation. Current eye research, 43(4).
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