M o m reagent

Manufactured by Vector Laboratories

The M.O.M. reagent is a laboratory product manufactured by Vector Laboratories. It is a chemical solution designed for use in specific laboratory applications. The core function of the M.O.M. reagent is to facilitate a particular technical process, but its intended use should not be extrapolated beyond the factual information provided.

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Lab products found in correlation

Paraformaldehyde (pfa), by Thermo Fisher Scientific (3 mentions) Nonfluorescent phalloidin, by Thermo Fisher Scientific (3 mentions) Donkey serum, by Jackson ImmunoResearch (3 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (3 mentions) Phem glycine, by Vector Laboratories (2 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

M.O.M. blocking reagent (26 citations) M.O.M mouse Ig blocking reagent (9 citations) Mouse on Mouse (M.O.M.) Blocking Reagent (5 citations) M.O.M™ mouse IgG blocking reagent (5 citations) M.O.MTM Mouse IgG blocking reagent (2 citations) Mouse on mouse reagent (M.O.M.; (2 citations) Mouse on Mouse (M.O.M.™) Blocking Reagent (MKB-2213, (2 citations)

3 protocols using m o m reagent

Immunocytochemistry Protocol for Cytoskeletal Proteins

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All fixation and permeabilization steps were performed at 37°C. Cells were permeabilized and fixed in 3% paraformaldehyde (Electron Microscopy Sciences), 0.5% Triton X-100 (Sigma), with 10 μg/ml nonfluorescent phalloidin (Invitrogen) in cytoskeletal buffer (CBS; 10 mM MES, 138 mM KCl, 2 mM EGTA, 3 mM MgCl₂ plus 5% sucrose) for 90 seconds, post-fixed in 4% paraformaldehyde in CBS for 15 minutes. Cells were rinsed 3X in PBS⁺⁺ and permeabilized with 0.5% Triton X-100 in CBS for 5 minutes. Cells were rinsed 5X over 40 minutes with PHEM+glycine (60 mM PIPES, 2 mM HEPES, 10 mM EGTA, 2 mM MgCl₂, 100 mM glycine, pH 6.9). Non-specific sites were blocked with 20% donkey serum (Jackson ImmunoResearch Laboratories), together with M.O.M. reagent (Vector Laboratories), in PHEM+glycine buffer for 1 hour. Cells were rinsed 3X with PHEM+glycine over 30 minutes. Primary and secondary antibodies were diluted in PHEM+glycine with 10% donkey serum and incubated for 45 and 25 minutes, respectively. Secondary antibodies were either from Jackson ImmunoResearch Laboratories or Molecular Probes.

Doyle A.D., Carvajal N., Jin A., Matsumoto K, & Yamada K.M. (2015). Local 3D matrix microenvironment regulates cell migration through spatiotemporal dynamics of contractility-dependent adhesions. Nature Communications, 6, 8720.

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Immunofluorescence Cytoskeleton Staining

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All fixation and permeabilization steps were performed at 37°C. Cells were permeabilized and fixed in 3% paraformaldehyde (Electron Microscopy Sciences), 0.5% Triton X-100 (Sigma), with 10 μg/ml nonfluorescent phalloidin (Invitrogen) in cytoskeletal buffer (CBS: 10 mM MES, 138 mM KCl, 2 mM EGTA, 3 mM MgCl₂ plus 5% sucrose) for 90 seconds, post-fixed in 4% paraformaldehyde in CBS for 15 min. Cells were rinsed 3X in PBS⁺⁺ and permeabilized with 0.5% Triton X-100 in CBS for 5 min. Cells were rinsed 5X over 40 min with PHEM+glycine (60 mM PIPES, 2 mM HEPES, 10 mM EGTA, 2 mM MgCl₂, 100 mM glycine, pH 6.9). Non-specific sites were blocked with 20% donkey serum (Jackson ImmunoResearch Laboratories), together with M.O.M. reagent (Vector Laboratories), in PHEM+glycine buffer for 1 h. Cells were rinsed 3X with PHEM+glycine over 30 min. For MDA-MB-231 cells, the combined fixation and permeabilization first step was skipped. Primary and secondary antibodies were diluted in PHEM+glycine with 10% donkey serum and incubated for 45 and 25 min, respectively. Secondary antibodies were from Jackson Immuno Research Laboratories.

Doyle A.D., Sykora D.J., Pacheco G.G., Kutys M.L, & Yamada K.M. (2021). 3D mesenchymal cell migration is driven by anterior cellular contraction that generates an extracellular matrix prestrain. Developmental cell, 56(6), 826-841.e4.

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Fixation and Permeabilization of Cells

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All fixation and permeabilization steps were performed at 37 °C. Cells were permeabilized and fixed in 3% paraformaldehyde (Electron Microscopy Sciences), 0.5% Triton X-100 (Sigma), with 10 μg ml⁻¹ nonfluorescent phalloidin (Invitrogen) in cytoskeletal buffer (CBS; 10 mM MES, 138 mM KCl, 2 mM EGTA, 3 mM MgCl₂ plus 5% sucrose) for 90 s, post-fixed in 4% paraformaldehyde in CBS for 15 min. Cells were rinsed 3X in PBS⁺⁺ and permeabilized with 0.5% Triton X-100 in CBS for 5 min. Cells were rinsed 5 × over 40 min with PHEM+glycine (60 mM PIPES, 2 mM HEPES, 10 mM EGTA, 2 mM MgCl₂, 100 mM glycine, pH 6.9). Non-specific sites were blocked with 20% donkey serum (Jackson ImmunoResearch Laboratories), together with M.O.M. reagent (Vector Laboratories), in PHEM+glycine buffer for 1 h. Cells were rinsed three times with PHEM+glycine over 30 min. Primary and secondary antibodies were diluted in PHEM+glycine with 10% donkey serum and incubated for 45 and 25 min, respectively. Secondary antibodies were either from Jackson ImmunoResearch Laboratories or Molecular Probes.

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