Gb12224

The immunohistochemistry of MPO was conducted using mouse anti-MPO antibodies (Servicebio, Wuhan, GB12224) and HRP-labeled goat anti-mouse antibodies (Servicebio, Wuhan, G23301) along with DAB chromogenic agent kit for histochemistry (Servicebio, Wuhan, GB12224). All procedures were carried out on the basis of the manufacturer’s instructions. After blocking the fixed tissue with serum, we added primary antibodies, secondary antibodies, and developer before staining the nuclei with DAPI. Afterwards, images of the tissue under the microscope were taken and analyzed. Hematoxylin-stained nuclei appeared blue, while MPO expression was marked by a brownish yellow color.

Myeloperoxidase (MPO) immunohistochemistry was performed using mouse anti‐MPO (Servicebio, GB12224) and HRP‐labelled goat anti‐mpO (Servicebio, G23301) antibodies and the histochemical DAB chromogenic agent kit (Servicebio, GB12224). All procedures were performed based on manufacturer's instructions. The fixed tissue was first blocked with serum. Subsequently, the primary antibody, secondary antibody and developer were added. Finally, the nuclei were stained with DAPI. Then, the tissue was examined under a microscope, and images were captured and analysed. The haematoxylin‐stained nuclei appeared blue, whereas positive mPO expression was indicated by a brownish yellow colour.

IHC and IF analyses were performed as described previously (49 (link)). Primary antibodies for IHC and IF were as followed: anti-MUC2 (GB14110; 1:500; Servicebio); anti-MPO (GB12224; 1:200; Servicebio); anti-Occludin (GB111401">GB111401; 1:200; Servicebio). Data were analyzed by Image J.