Log In Sign up
The largest database of trusted experimental protocols

Biotinylated griffonia simplicifolia lectin 1 isolectin b4

Manufactured by Vector Laboratories
Visit Supplier
Sourced in United Kingdom

Biotinylated Griffonia Simplicifolia Lectin I-Isolectin B4 is a lectin purified from the seeds of the Griffonia simplicifolia plant. It is a specific marker for endothelial cells and their precursors.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti mouse collagen 4, by Bio-Rad (2 mentions) Goat anti rabbit alexfluor 594, by Thermo Fisher Scientific (2 mentions) Eclipse te200 u, by Nikon (2 mentions) Fitc conjugated streptavidin, by Agilent Technologies (2 mentions) Histopaque 1083, by Merck Group (1 mentions) Rat igg2a k isotype control apc, by Thermo Fisher Scientific (1 mentions) Egm 2 medium, by Lonza (1 mentions) Alexa fluor 488 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions) Isolectin b4, by Vector Laboratories (1 mentions) Streptavidin fitc, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Biotinylated lectins (43 citations) Isolectin B4 (42 citations) Biotinylated isolectin B4 (27 citations) Griffonia Simplicifolia Lectin I (10 citations) Lectins (7 citations) Simplicifolia lectin 1 (4 citations) Biotinylated griffonia ( (4 citations) Griffonia simplicifolia isolectin B4 (2 citations) Biotinylated griffonia simplicifolia lectin (2 citations) Lectin I isolectin B4 (2 citations)

5 protocols using biotinylated griffonia simplicifolia lectin 1 isolectin b4

1

Retinal Pigment Epithelium and Choroid Imaging

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RPE/choroidal whole mounts were prepared as described previously [43 (link)]. Briefly, tissues were permeabilised with 0.3% triton X-100 for 1h. The samples were then blocked with 6% BSA and incubated with biotinylated Griffonia Simplicifolia Lectin I-Isolectin B4 (1:100, Vector Laboratories Ltd., Peterborough, UK) and rabbit anti-mouse collagen IV (1:100, ABD Serotec Ltd, Oxford, UK), followed by FITC-conjugated Streptavidin (1:200, Dako, Denmark) and goat anti-rabbit AlexFluor 594 (1:200, Invitrogen, Paisley, UK) for 2h. Samples were observed by confocal microscopy (Eclipse TE200-U, Nikon UK Ltd., Surry, UK).
Chen M., Lechner J., Zhao J., Toth L., Hogg R., Silvestri G., Kissenpfennig A., Chakravarthy U, & Xu H. (2016). STAT3 Activation in Circulating Monocytes Contributes to Neovascular Age-Related Macular Degeneration. Current Molecular Medicine, 16(4), 412-423.
+ Open protocol
+ Expand
2

Isolation and Analysis of Murine Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
EGM-2 Medium was purchased from Lonza corporation (CC-3202). Histopaque®-1083 was from Sigma-Aldrich (10831). FAM-YVAD-FMK caspase-1 detection kit was from Cell Technology (FAM600-2). Anti-APC MultiSort kit and MicroBeads were from MACS (130-091-255/130-048-801). Rat IgG2a K Isotype control APC was from eBioscience (17-4321-81). Biotinylated Griffonia simplicifolia lectin I (isolectin B4) from Vector (B-1205), Biotin-XX conjugate was from Invitrogen (I21414). APC anti-mouse Ly-6A/E (Sca-1) antibody were from Biolegend (121906/108112). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO).
Li Y.F., Huang X., Li X., Gong R., Yin Y., Nelson J., Gao E., Zhang H., Hoffman N.E., Houser S.R., Madesh M., Tilley D.G., Choi E.T., Jiang X., Huang C.X., Wang H, & Yang X.F. (2016). Caspase-1 mediates hyperlipidemia-weakened progenitor cell vessel repair. Frontiers in bioscience (Landmark edition), 21, 178-191.
+ Open protocol
+ Expand
3

Isolectin B4 Staining Technique

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolectin B4 staining was performed by incubating sections with Biotinylated Griffonia Simplicifolia Lectin I Isolectin B4 (Vector Laboratories) followed by detection using Alexa Fluor 488-conjugated streptavidin (Invitrogen).
Rowan S., Jiang S., Chang M.L., Volkin J., Cassalman C., Smith K.M., Streeter M.D., Spiegel D.A., Moreira-Neto C., Rabbani N., Thornalley P.J., Smith D.E., Waheed N.K, & Taylor A. (2020). A low glycemic diet protects disease-prone Nrf2-deficient mice against age-related macular degeneration. Free radical biology & medicine, 150, 75-86.
+ Open protocol
+ Expand
4

Mouse Eye Retinal Flatmount Staining

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole mouse eyes were collected and fixed in 2% PFA/PBS for 2 h before transferring to PBS and stored in 4 °C for further processing [20 (link)]. For retinal flatmount staining, retinal tissues were permeabilised and blocked in 1% BSA/PBS for 2 h and then incubated in primary antibodies (Biotinylated Griffonia Simplicifolia Lectin I Isolectin B4: 1:50, Vector laboratory, Peterborough, UK; rabbit anti-mouse Collagen IV: 1:50, AbD Serotec®, Kidlington, UK) overnight. After thorough washes, samples were incubated with secondary antibodies (Streptavidin FITC or goat anti-rabbit Alex Fluor 569, all from Invitrogen, UK, 1:200) at room temperature for 2 h. Tissues were flatmounted on glass slides with mounting medium and evaluated under confocal microscope (EZ-C1 confocal system, Nikon UK Limited, Surrey, UK).
Chen M., Obasanmi G., Armstrong D., Lavery N.J., Kissenpfennig A., Lois N, & Xu H. (2019). STAT3 activation in circulating myeloid-derived cells contributes to retinal microvascular dysfunction in diabetes. Journal of Neuroinflammation, 16, 138.
+ Open protocol
+ Expand
5

Retinal Pigment Epithelium and Choroid Imaging

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
RPE/choroidal whole mounts were prepared as described previously.[43 (link)] Briefly, tissues were permeabilised with 0.3% triton X-100 for 1h. The samples were then blocked with 6% BSA and incubated with biotinylated Griffonia Simplicifolia Lectin I-Isolectin B4 (1:100, Vector Laboratories Ltd, Peterborough, UK) and rabbit anti-mouse collagen IV (1:100, ABD Serotec Ltd, Oxford, UK), followed by FITC-conjugated Streptavidin (1:200, Dako, Denmark) and goat anti-rabbit AlexFluor 594 (1:200, Invitrogen, Paisley, UK) for 2h. Samples were observed by confocal microscopy (Eclipse TE200-U, Nikon UK Ltd, Surry, UK).
Chen M., Lechner J., Zhao J., Toth L., Hogg R., Silvestri G., Kissenpfennig A., Chakravarthy U, & Xu H. (2016). STAT3 Activation in Circulating Monocytes Contributes to Neovascular Age-Related Macular Degeneration. Current Molecular Medicine, 16(4), 412-423.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!