To measure 8-OHG engulfment by DCs, apoEG7 cells were cultured with BMDCs at a 1:1 ratio for 3 h in vitro.15 (link) In vivo, mice were injected intraperitoneally with irradiated EG7 cells and housed for 18 h. Cells in the peritoneal lavage fluid and BMDCs stimulated with EG7 cells were stained with goat anti-8-OHG (1:300; Abcam), Alexa Fluor® 647-rabbit anti-goat IgG (1:1000; Thermo Fisher), hamster anti-CD11c (1:200; #117301; BioLegend) and FITC-rabbit anti-hamster IgG (1:1000; Thermo Fisher) antibodies and observed under a Leica TCS SP5 confocal microscope after staining with DAPI. Moreover, cells in the peritoneal lavage fluid were stained with anti-CD11c, anti-CD45 and anti-8-OHG antibodies and analyzed by flow cytometry. BMDCs stimulated with EG7 cells were stained with anti-CD11c and anti-8-OHG antibodies and detected by flow cytometry.
Hamsteranti cd11c
Manufactured by BioLegend
Hamster anti-CD11c is a monoclonal antibody that recognizes the CD11c antigen, which is expressed on the surface of dendritic cells and a subset of monocytes and macrophages. This antibody can be used for the identification and enumeration of these cell populations in flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti cd3, by Abcam (2 mentions)
Hrp conjugated secondary antibody, by Vector Laboratories (2 mentions)
3 3 diaminobenzidine (dab), by Vector Laboratories (2 mentions)
Goat anti rat igg, by Vector Laboratories (2 mentions)
Hematoxylin, by StatLab (2 mentions)
Horse anti rabbit igg, by Vector Laboratories (2 mentions)
Rat anti cd8, by BioLegend (2 mentions)
Goatanti hamster igg, by Vector Laboratories (2 mentions)
Tcs sp5 confocal microscope, by Leica (1 mentions)
Alexa fluor 647 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Goat anti 8 ohg, by Abcam (1 mentions)
3 protocols using hamsteranti cd11c
1
Measuring 8-OHG Engulfment by Dendritic Cells
2
Immunohistochemical Analysis of Vaginal Immune Cells
3
Immunohistochemical Analysis of Vaginal Immune Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The vagina was embedded in OCT and snap frozen in liquid nitrogen
pre-cooled isopentane. Frozen vaginal tissue blocks were cut into sections with
the thickness of 5 μm, stored in −70°C until use.
Cryostat sections were fixed in acetone for 5 min, rehydrated in PBS, and
incubated overnight with primary antibodies including rabbit anti-CD3 (1:100
dilution, AbCam), rat anti-CD8 (1:200 dilution, Biolegend) and hamster
anti-CD11c (1:150 dilution, Biolegend). The primary antibodies were washed out 3
times by PBS followed by the addition of HRP conjugated secondary antibodies
(Vector Lab) including horse anti-rabbit IgG, goat anti-rat IgG and goat
anti-hamster IgG. The secondary antibodies were incubated in room temperature
for 30 minutes followed by DAB (kit from Vector Lab) development and hematoxylin
(BBC Biochemical) counter staining.
pre-cooled isopentane. Frozen vaginal tissue blocks were cut into sections with
the thickness of 5 μm, stored in −70°C until use.
Cryostat sections were fixed in acetone for 5 min, rehydrated in PBS, and
incubated overnight with primary antibodies including rabbit anti-CD3 (1:100
dilution, AbCam), rat anti-CD8 (1:200 dilution, Biolegend) and hamster
anti-CD11c (1:150 dilution, Biolegend). The primary antibodies were washed out 3
times by PBS followed by the addition of HRP conjugated secondary antibodies
(Vector Lab) including horse anti-rabbit IgG, goat anti-rat IgG and goat
anti-hamster IgG. The secondary antibodies were incubated in room temperature
for 30 minutes followed by DAB (kit from Vector Lab) development and hematoxylin
(BBC Biochemical) counter staining.
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