Hrp conjugated anti mouse igg each isotype antibody

Manufactured by Southern Biotech

Sourced in United States

The HRP-conjugated anti-mouse IgG (each isotype) antibody is a laboratory reagent designed for use in various immunoassay techniques. It is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP), an enzyme used for signal detection.

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Lab products found in correlation

96 well immunoplate, by Thermo Fisher Scientific (2 mentions) Spectramax 250 microplate reader, by Molecular Devices (1 mentions)

2 protocols using hrp conjugated anti mouse igg each isotype antibody

Quantifying MERS-CoV Spike Protein Antibodies

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To measure the epitope peptide-specific antibody titer, 96-well immunoplates (Thermo Fisher Scientific Co.) were coated with 5 μg/well of the MERS-CoV Spike-492 or Spike-492 (L506F) peptides. The mice sera were obtained by retro orbital bleeding and analyzed as previously described (22 (link)). To identify the isotype of the monoclonal antibody, the HRP-conjugated anti-mouse IgG (each isotype) antibody (Southern Biotechnology Associates Inc.) was used. For detection of cross-reactivity, 96-well immunoplates were coated with the MERS-CoV Spike-492 or Spike-492 (L506F) peptides and incubated with either 492-1G10E4E2 or 506-2G10G5 monoclonal antibody for 2 h before incubation with the secondary antibody. The absorbance was evaluated with the Spectra Max 250 microplate reader (Molecular Devices Co.) at 450 nm and then calculated with the SigmaPlot program to determine the affinity constant (EC50 value) as described previously (17 (link)).

Park B.K., Maharjan S., Lee S.I., Kim J., Bae J.Y., Park M.S, & Kwon H.J. (2019). Generation and characterization of a monoclonal antibody against MERS-CoV targeting the spike protein using a synthetic peptide epitope-CpG-DNA-liposome complex. BMB Reports, 52(6), 397-402.

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MERS-M158 Peptide Antibody Quantification

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The MERS-M158 peptide was coated at 5 µg/well onto 96-well immunoplates (Thermo Fisher Scientific) and then blocked with PBST (PBS supplemented with 0.05% Tween-20) containing 1% BSA. Mouse sera were collected by orbital bleeding to measure the epitope peptide-specific antibody production. The epitope peptide-specific antibody levels in the mouse sera, hybridoma culture supernatants and ascites as well as the purified monoclonal antibody were quantified as previously described (Park et al. 2015 (link)). The isotype of the monoclonal antibody was identified with HRP-conjugated anti-mouse IgG (each isotype) antibody (Southern Biotech, Birmingham, AL, USA).

Park B.K., Lee S.I., Bae J.Y., Park M.S., Lee Y, & Kwon H.J. (2018). Production of a Monoclonal Antibody Targeting the M Protein of MERS-CoV for Detection of MERS-CoV Using a Synthetic Peptide Epitope Formulated with a CpG–DNA–Liposome Complex. International Journal of Peptide Research and Therapeutics, 25(3), 819-826.

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