S‐region sequences were used to determine the genotype of the HBV by comparing them to reference strains using Bionumerics version 7.1 (Sint‐Martens‐Latem, Belgium).36 Phylogenetic trees were constructed using the maximum parsimony method. To assess genetic variation within the HBsAg and RT, we analyzed the appearance and frequency of amino acid sequence variation within the S and RT genes by type by aligning all sequences using Bionumerics version 7.1 to the sequences of the reference HBV types (A to J) assigned by Pourkarim et al.7 Subgenotype‐specific polymorphisms were excluded in the analysis. All sequences were deposited in GenBank (accession numbers: KX659172 to KX659981 and KP243601 to KP244031). For clarity, strains isolated from acute infected cases are referred to as acute strains and strains isolated from chronic cases, as chronic strains.
Version 7.1
Manufactured by BioNumerics
Sourced in Belgium
BioNumerics (version 7.1) is a software application for the analysis and management of biological and numerical data. It provides tools for tasks such as data entry, storage, analysis, and visualization of various types of biological information.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using version 7.1
1
Genotyping HBV Using S-region Sequences
2
ERIC-PCR Dendrogram Analysis of E. coli and K. pneumoniae
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The DNA band pattern of the E. coli and K. pneumoniae samples obtained using gel electrophoresis of ERIC-PCR products was used as a generational structure for dendrogram analysis using BioNumerics version 7.1. For constructing a computerized dendrogram, the presence and absence of bands were presumed as 1 and 0, respectively. The dendrogram was then designed using the unweighted pair-group method with arithmetic mean, which is categorized in clustering methodologies and is based on clustering analysis.
3
Multilocus Sequence Typing of Bacterial Isolates
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All isolates were subjected to MLST according to the protocols described in the EcMLST database (http://www.shigatox.net/ecmlst). The PCR products were bidirectionally sequenced, and the sequences of the 15 housekeeping genes were edited by using SeqMan 7.0. Each unique allele was assigned a different number, and the allelic pro le (string of fteen allelic loci) was used to de ne each isolate's sequence type (ST) [48] . Clustering and minimum spanning tree (MST) analyses were used to infer relationships among the isolates using the ngerprint analysis software BioNumerics (version 7.1).
4
Multilocus Sequence Typing of Isolates
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All isolates were subjected to MLST according to the protocols described in the EcMLST database (http://www.shigatox.net/ecmlst). The PCR products were bidirectionally sequenced, and the sequences of the 15 housekeeping genes were edited by using SeqMan 7.0. Each unique allele was assigned a different number, and the allelic pro le (string of fteen allelic loci) was used to de ne each isolate's sequence type (ST) [26] . Clustering and minimum spanning tree (MST) analyses were used to infer relationships among the isolates using the ngerprint analysis software BioNumerics (version 7.1).
5
Multilocus Sequence Typing of Bacterial Isolates
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All isolates were subjected to MLST according to the protocols described in the EcMLST database (http://www.shigatox.net/ecmlst). The PCR products were bidirectionally sequenced, and the sequences of the 15 housekeeping genes were edited by using SeqMan 7.0. Each unique allele was assigned a different number, and the allelic pro le (string of fteen allelic loci) was used to de ne each isolate's sequence type (ST) [48] . Clustering and minimum spanning tree (MST) analyses were used to infer relationships among the isolates using the ngerprint analysis software BioNumerics (version 7.1).
6
Multilocus Sequence Typing of Bacterial Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All isolates were subjected to MLST according to the protocols described in the EcMLST database (http://www.shigatox.net/ecmlst). The PCR products were bidirectionally sequenced, and the sequences of the 15 housekeeping genes were edited by using SeqMan 7.0. Each unique allele was assigned a different number, and the allelic pro le (string of fteen allelic loci) was used to de ne each isolate's sequence type (ST) [48] . Clustering and minimum spanning tree (MST) analyses were used to infer relationships among the isolates using the ngerprint analysis software BioNumerics (version 7.1).
7
Multilocus Sequence Typing of Bacterial Isolates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All isolates were subjected to MLST according to the protocols described in the EcMLST database (http://www.shigatox.net/ecmlst). The PCR products were bidirectionally sequenced, and the sequences of the 15 housekeeping genes were edited by using SeqMan 7.0. Each unique allele was assigned a different number, and the allelic pro le (string of fteen allelic loci) was used to de ne each isolate's sequence type (ST) [48] . Clustering and minimum spanning tree (MST) analyses were used to infer relationships among the isolates using the ngerprint analysis software BioNumerics (version 7.1).
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