Animal experiments were carried out using protocols approved by the Animal Care Committee at the University of Ottawa and conforming to or exceeding the standards defined by the Canadian Council on Animal Care. FVB/N mice (for STOSE and MOE cell lines) were acquired from Charles River and C57BL/6 mice (for ID8 and derivatives) were purchased from Jackson Laboratories. Intrabursal (IB) injections of cell lines (0.15 × 106 cells) were performed as previously described (20 (link)). Intraperitoneal (IP) injections of 5 × 106 cells were delivered to a subset of the mice. As the mice developing IB tumors neared humane endpoint (4–5 days before anticipated endpoint), primary tumors, abdominal metastases, spleens, and ascites were collected for flow cytometry, immunohistochemistry (frozen and fixed), immunofluorescence, qPCR, and single-cell RNA sequencing analysis.
Fvb n mice for stose and moe cell lines
Manufactured by Charles River Laboratories
FVB/N mice are a commonly used strain of laboratory mice. They are suitable for the generation and maintenance of STOSE and MOE cell lines.
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Lab products found in correlation
2 protocols using fvb n mice for stose and moe cell lines
1
Murine Model of Ovarian Cancer
2
Intrabursal Ovarian Tumor Xenograft Model
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Animal experiments were carried out using protocols approved by the Animal Care Committee at the University of Ottawa and conforming to or exceeding the standards defined by the Canadian Council on Animal Care. FVB/N mice (for STOSE and MOE cell lines) were acquired from Charles River and C57BL/6 mice (for ID8 and derivatives) were purchased from Jackson Laboratories. Intrabursal injections of cell lines (0.15 × 106 cells) were performed as previously described (20 (link)). Intraperitoneal injections of 5 × 106 cells were delivered to a subset of the mice. As the mice developing intrabursal tumors neared humane endpoint (4–5 days before anticipated endpoint), primary tumors, abdominal metastases, spleens, and ascites were collected for flow cytometry, IHC (frozen and fixed), immunofluorescence, qPCR, and single-cell RNA-sequencing analysis.
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