Lungs were lavaged with 1 mL ice-cold PBS via an intratracheal tube. BAL cell counts were determined. Differential cell counts of at least 200 counted cells for macrophages, lymphocytes, neutrophils, and eosinophils were performed on cytocentrifuged preparations stained with Hemacolor-Set (Merck). From these, percentages and absolute cell counts were calculated.
Hemacolor set
Manufactured by Merck Group
The Hemacolor-Set is a laboratory equipment product from Merck Group. It is designed for the rapid staining of blood smears for microscopic examination. The Hemacolor-Set comprises a set of solutions that enable the differentiation and identification of various blood cell types.
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Lab products found in correlation
2 protocols using hemacolor set
1
Bronchoalveolar Lavage Cell Analysis
2
Differential Cell Count in BAL
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Lungs were flushed with 1 mL ice-cold PBS through an i.t. tube and total cell counts were determined. To determine differential cell counts, cytocentrifuged preparations of BAL were stained with Hemacolor-Set (Merck) and at least 200 cells were counted and differentiated into macrophages, lymphocytes, neutrophils and eosinophils based on histological properties. The total cell count for each cell type was calculated based on relative cell counts and the initially determined total cell number.
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