Way 262 611

Pae purchased from MedChemExpress (HY-N0293, Monmouth Junction, NJ, USA) was diluted into a 1 mg/mL solution with DMSO. The lactose dehydrogenase (LDH) assay kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China, C0016) and the Caspase-3 kit from Abcam Inc. (ab39401, Cambridge, UK). The primary antibodies used in Western blotting analysis included FOXO1 (2880; Cell Signaling Technologies, Beverly, MA, USA), GSK3β (ab32391, Abcam), GSK3β (phosphoY216, ab75745, Abcam), and β-catenin (8480, Cell Signaling Technologies). The secondary antibody was horseradish peroxidase-conjugated goat anti-rabbit antibody against IgG (ab6721, Abcam). miR-15b mimic and mimic control were purchased and synthesized by Sangon (Shanghai, China). A siRNA and a pcDNA3.1 overexpression plasmid targeting FOXO1 were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). The β-catenin signaling agonist WAY-262,611 was purchased from MedChemExpress (HY-11,035) and was diluted into 5 μM solution. The GSK3β-specific inhibitor lithium chloride (LiCl) was purchased from Abcam (ab120853). 4-OHT was purchased from Sigma-Aldrich (68,392-35-8) and was dissolved in methanol in concentrations ranging from (0.01–10 μM).