Intestinal samples were dehydrated in a graded ethanol series (50, 70, 80, 90, 95, and 100% ethanol), clarified with fresh xylene, and then embedded into paraffins. About 5 μm intestinal cross sections were prepared and stained with common haematoxylin-eosin (HE ) staining (Chen et al., 2015 ) for morphology measurement. The images of stained samples were captured using an Olympus DP12 CCD digital camera (Olympus Optical Co. Ltd., Tokyo, Japan) under a light microscope at 40× magnification (Olympus BX41, Olympus Optical Co. Ltd.). Then, the villus height (VH ; from the tip of the villi to the villi crypt opening) and the crypt depth (CD ; from the base of the crypt to the level of the crypt opening) were determined by the Image-Pro Plus 6.0 software (Media Cybernetics, Bethesda, MD, USA), the ratio of VH to crypt depth (VH/CD ) was then calculated. At least 6 well-oriented, intact villus-crypt units of intestinal (duodenum, jejunum and ileum) cross section from one section per chicken were examined. The average value of the 6 structures per chicken was used.
Dp12 ccd digital camera
Manufactured by Olympus
Sourced in Japan
The DP12 CCD digital camera is a professional-grade imaging device designed for scientific and industrial applications. It features a high-resolution CCD sensor that captures detailed and accurate digital images. The camera is capable of producing high-quality images with low noise and excellent color fidelity. The DP12 is suitable for a wide range of microscopy and imaging applications where accurate and reliable image capture is required.
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Lab products found in correlation
2 protocols using dp12 ccd digital camera
1
Intestinal Morphology Measurement Protocol
2
Intestinal Histomorphometry Analysis
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Intestinal samples were embedded in paraffin and cross sections (5 μm) were cut and stained with hematoxylin–eosin (HE) staining. Images were captured using an Olympus DP12 CCD digital camera (Olympus Optical Co. Ltd, Tokyo, Japan) and analyzed by Image-Pro Plus 6.0 software (Media Cybernetics, Bethesda, MD, USA) to measure the VH (μm, from the tip of villus to the villus-crypt junction level for 10 villi per section) and CD (μm, the vertical distance from the villus-crypt junction to the lower limit of the crypt for 10 corresponding crypts per section). The ratio of VH to CD (μm/μm) was also calculated.
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