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Mouse ifn γ elisa max deluxe kit

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The Mouse IFN-γ ELISA MAX™ Deluxe kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of mouse interferon-gamma (IFN-γ) in cell culture supernatants, serum, and plasma samples.

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Lab products found in correlation

Human ifn γ quantikine elisa kit, by R&D Systems (2 mentions) Human il 18 bpaquantikine elisa kit, by R&D Systems (2 mentions) Mouse il 18bpd duoset elisa kit, by R&D Systems (2 mentions) Primate ifn γ duoset elisa, by R&D Systems (2 mentions) Facscalibur, by BD (1 mentions) Softmax pro software, by Molecular Devices (1 mentions) Complete protease inhibitor cocktail, by Merck Group (1 mentions)

Close spelling variants of this product

IFN-γ ELISA MAX Deluxe kit Mouse IFN-γ ELISA MAX Mouse IFN-γ ELISA ELISA MAX Deluxe IFN-γ ELISA kit IFN-γ ELISA Mouse IFN-γ ELISA MAX kits IFN-γ Mouse ELISA kits

5 protocols using mouse ifn γ elisa max deluxe kit

1

Quantitative Cytokine Measurements

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IL-18BP and IFN-γ ELISAs were performed using Human IL-18 BPa
Quantikine ELISA Kit (R&D system, #DBP180), mouse IL-18BPd DuoSet ELISA kit
(R&D system, DY122-05), mouse IFN-γ ELISA MAX™ Deluxe kit
(Biolegend, #430804), human IFN-γ Quantikine ELISA Kit (R&D system,
#DIF150), and primate IFN-γ DuoSet ELISA (R&D system, #DY961)
according to the manufacturers’ instructions.
Zhou T., Damsky W., Weizman O.E., McGeary M.K., Hartmann K.P., Rosen C.E., Fischer S., Jackson R., Flavell R.A., Wang J., Sanmamed M.F., Bosenberg M.W, & Ring A.M. (2020). IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature, 583(7817), 609-614.
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2

Intracellular Cytokine Profiling of Mouse Immune Cells

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Intracellular cytokine staining followed by flow cytometry was performed on splenocytes and lymphocytes isolated from mouse lungs as described above. All reagents were obtained from BD Pharmingen (San Diego, CA). Following 18hrs of stimulation, GolgiPlug was added to all wells. At 24hrs cells were collected for staining. Following CD16/CD32 blocking with Fc Block, cell surfaces were stained with PE conjugated α-mouse CD4 antibody, clone RM4-5 and PerCP conjugated α-mouse CD8 antibody, clone 53-6.7. Cells were fixed and permeabilized with Cytofix/Cytoperm and stained intracellularly with APC conjugated α-mouse IFN-γ antibody, clone XMG1.2 and FITC conjugated α-mouse IL-17a, clone eBiol7B7 (eBioscience, San Diego, CA). Cells were analyzed using a BD FACSCalibur and data was analyzed using FlowJo (FlowJo, LLC, Ashland, OR). The supernatant of the same samples was analyzed by enzyme-linked immunosorbent assay (ELISA) for IFN-γ levels using the Mouse IFN-γ ELISA MAX™ Deluxe kit following the manufacturer's instructions (Biolegend, San Diego, CA). Readings were acquired and analyzed with SoftMax Pro software (Molecular Devices, Sunnyvale, CA).
Marcus S.A., Steinberg H, & Talaat A.M. (2015). Protection by novel vaccine candidates, Mycobacterium tuberculosis ΔmosR and ΔechA7, against challenge with a Mycobacterium tuberculosis Beijing strain. Vaccine, 33(42), 5633-5639.
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3

Quantification of Lung IFN-γ in IAV Infection

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To determine IFN-γ levels within lungs of IAV-infected mice, lungs were isolated and stored in 1 ml sterile PBS at −70 °C until homogenized. For homogenization, samples were thawed, diluted in 4 ml sterile PBS containing cOmplete™ Protease Inhibitor Cocktail (Merck) and processed using a homogenizator. After centrifugation, supernatants were stored at −70 °C until measurement using the Mouse IFN-γ ELISA MAX Deluxe kit (BioLegend) following the instructions of the manufacturer.
Milanez-Almeida P., Ulas T., Pasztoi M., Glage S., Schughart K., Lutz M.B., Schultze J.L, & Huehn J. (2015). CD11b+Ly6C++Ly6G- Cells with Suppressive Activity Towards T Cells Accumulate in Lungs of Influenza a Virus-Infected Mice. European Journal of Microbiology & Immunology, 5(4), 246-255.
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4

Quantitative Cytokine Measurements

Check if the same lab product or an alternative is used in the 5 most similar protocols
IL-18BP and IFN-γ ELISAs were performed using Human IL-18 BPa
Quantikine ELISA Kit (R&D system, #DBP180), mouse IL-18BPd DuoSet ELISA kit
(R&D system, DY122-05), mouse IFN-γ ELISA MAX™ Deluxe kit
(Biolegend, #430804), human IFN-γ Quantikine ELISA Kit (R&D system,
#DIF150), and primate IFN-γ DuoSet ELISA (R&D system, #DY961)
according to the manufacturers’ instructions.
Zhou T., Damsky W., Weizman O.E., McGeary M.K., Hartmann K.P., Rosen C.E., Fischer S., Jackson R., Flavell R.A., Wang J., Sanmamed M.F., Bosenberg M.W, & Ring A.M. (2020). IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy. Nature, 583(7817), 609-614.
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5

Quantifying Cytokine Levels in BMDC and Tumor Samples

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Cell-free supernatant from BMDC cultures or BMDC/T-cell co-cultures was harvested at the indicated time points. Concentration of IL-12/p70 or IFNγ was measured. To measure cytokine concentrations in sera, blood samples were collected from WT and miR-155−/− mice bearing breast tumors and allowed to clot for 30 min at room temperature; the samples were then centrifuged at 3,000 × g for 10 min; the serum layer was removed and diluted 1:5. Cytokine concentrations were determined by Mouse IL-12 (p70) ELISA MAX™ Deluxe kit (Biolegend, 433604) and Mouse IFNγ ELISA MAX™ Deluxe kit (Biolegend, 430804) according to the manufacturer's instructions. All samples were tested in triplicate.
Wang J., Iwanowycz S., Yu F., Jia X., Leng S., Wang Y., Li W., Huang S., Ai W, & Fan D. (2016). microRNA-155 deficiency impairs dendritic cell function in breast cancer. Oncoimmunology, 5(11), e1232223.
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