Recombinant mcherry

Manufactured by Abcam

Recombinant mCherry is a fluorescent protein derived from the Discosoma species of coral. It is a red fluorescent protein that can be used as a marker or reporter in various biological applications.

Automatically generated - may contain errors

Lab products found in correlation

Nigericin, by Merck Group (1 mentions) Human fibrinogen, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions) Peptidoglycan from staphylococcus aureus, by InvivoGen (1 mentions) Ac yvad cmk, by Enzo Life Sciences (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Pam3csk4, by InvivoGen (1 mentions) Pitstop 2, by Abcam (1 mentions)

2 protocols using recombinant mcherry

Quantifying CXCL12 binding to astrocytes

Cited 1 time

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Astrocytes were seeded in 24-well plates until confluent and treated with DMSO or CCX771 for 2 h. CXCL12-mCherry fusion protein (Cruz-Orengo et al., 2011b (link)) or recombinant mCherry (BioVision) was then added to the cultures for 4 h. Cells were then washed with cold 0.5 M acetic acid wash buffer to remove surface bound CXCL12-mCherry, and then fixed with ice-cold 4% PFA. Astrocytes were then labeled with polyclonal rabbit anti-DsRed (1:200; Takara Bio Inc.) and counterstained with ToPro3 as previously described (Cruz-Orengo et al., 2011b (link)).

Williams J.L., Patel J.R., Daniels B.P, & Klein R.S. (2014). Targeting CXCR7/ACKR3 as a therapeutic strategy to promote remyelination in the adult central nervous system. The Journal of Experimental Medicine, 211(5), 791-799.

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Investigating Innate Immune Responses

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Lipopolysaccharide from E. coli Serotype EH100(Ra) was purchased from Enzo. Lipoteichoic acid from Bacillus subtilis, peptidoglycan from Staphylococcus aureus, R848, and Pam3CSK4 were purchased from Invivogen. Ac-YVAD-CMK (inhibitor of caspase-1) and CRID 3 (inhibitor of NLRP3) were purchased from Enzo and Tocris, respectively. Recombinant mCherry was purchased from BioVision. Pitstop-2 was purchased from Abcam. Wortmannin, methyl-β-cyclodextrin, ATP, nigericin and human fibrinogen were purchased from Sigma.

Valderrama J.A., Riestra A.M., Gao N.J., LaRock C.N., Gupta N., Ali S.R., Hoffman H.M., Ghosh P, & Nizet V. (2017). Group A Streptococcal M Protein Activates the NLRP3 Inflammasome. Nature microbiology, 2(10), 1425-1434.

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