Th e kits used were ELISA diagnostic kits of two types. First, the screening diagnostic kit was used in order to identify samples positive for antibodies against SRLV. Positive samples identifi ed with screening ELISA kit (ID Screen MVV-CAEV Indirect Screening test, IDvet, France) were then analysed with a conformation ELISA kit (INgezim MAEDI Confi rmation, Ingenaza, Spain) in order to obtain the conformation of positive samples. Screening kit is designed to detect antibodies against SRLV in sheep and goat serum samples, based on an indirect ELISA technique using a purifi ed pool of SRLV antigenic peptides. Conformation assay was designed to confi rm the positive samples obtained in the screening test by detection of specifi c antibodies against SRLV through the use of specifi c peptides of genotypes A and B. Th e sensitivity and specifi city of the kits were 95%.
Id screen mvv caev indirect screening test
Manufactured by IDvet
Sourced in France
The ID Screen MVV/CAEV Indirect Screening test is a diagnostic tool designed to detect the presence of antibodies against Maedi-Visna virus (MVV) and Caprine Arthritis Encephalitis Virus (CAEV) in serum or plasma samples. The test employs an indirect ELISA (Enzyme-Linked Immunosorbent Assay) format, providing a reliable and efficient method for the screening of these viral infections in small ruminants.
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Lab products found in correlation
6 protocols using id screen mvv caev indirect screening test
1
ELISA-Based Detection of SRLV Antibodies
2
SRLV Serological Status Determination
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The serological status of SRLV infection was determined using the ELISA test (ID Screen MVV/CAEV Indirect Screening test, IDVet, Grabels, France).
3
Carpathian Goat Blood Profiling
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Whole blood was taken from 27 adult goats by jugular venipuncture and stabilized in ethylenediaminetetraacetic acid (EDTA) and Tempus blood RNA tubes. Goats represented the Carpathian breed, and they were owned by the National Research Institute of Animal Production in Krakow. All goats were healthy and maintained in one flock in the same environment. This involved being housed indoors in sheds, aside from during the grazing season (April–November), where they spent daily hours in pastures outdoors. It also involved the feeding conditions (summer feeding based on pasture or green fodder and winter feeding consisting mainly of hay and oats). Serological status of animals for SRLV infection was confirmed by enzyme-linked immunosorbent assay (ELISA) (ID Screen MVV/CAEV Indirect Screening test, IDVet, Grabels, France) according to the manufacturer’s recommendations. Blood samples were collected from all animals on the same day. At the time when blood was taken, none of the goats exhibited any clinical signs of the disease. All procedures associated with animal handling and treatments were approved (no 37/2016) by the Local Ethical Committee on Animal Testing at the University of Life Sciences in Lublin (Poland).
4
SRLV Antibody Detection Protocol
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Serological tests were performed in duplicate on serum samples using the commercial ELISA assay (ID Screen® MVV/CAEV Indirect Screening test–IDvet, Grabels, France) that detects gp135 and p25 antibodies, following the manufacturer’s instructions. Raw data were acquired by the SunriseTM Basic Tecan spectrometer equipped with a MagellanTM reader control and data processing software v7.1 (Tecan Group Ltd., Männedorf, Switzerland). The cut-off value was defined based on the corrected optical density (OD) ratio between sample and positive control (S/P) at a wavelength of 450 nm:
According to the manufacturer’s instructions, samples are considered SRLVs negative with an S/P value ≤50% and SRLVs positive with an S/P value ≥60%. Doubtful results, given with a value in the range of 50–60%, were excluded from further analysis.
According to the manufacturer’s instructions, samples are considered SRLVs negative with an S/P value ≤50% and SRLVs positive with an S/P value ≥60%. Doubtful results, given with a value in the range of 50–60%, were excluded from further analysis.
5
Serological Screening for SRLV in Animals
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The serological status of animals for SRLV infection was determined using the commercially available ID Screen MVV/CAEV Indirect Screening test (IDVet, France) according to the manufacturer’s recommendations. Additionally, sera were tested by ELISA based on Gag and Env multi-epitope recombinant antigens [18 (link)]. These antigens included either the Gag domain, containing nearly the whole matrix (MA) and capsid (CA) proteins fused to the SU1 and SU5 antigenic sites of the surface glycoprotein (SU)–(SU1/Gag/SU5 antigen), or the SU1 and SU5 antigenic sites only (without the Gag domain)–(SU1/SU5 antigen). Both antigens were developed on the basis of subtypes A1, A13, B1 and B2 of SRLV, which had been confirmed to circulate in small ruminants in Poland [18 (link)].
6
Serum-based Screening for Small Ruminant Lentivirus
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Blood samples were drawn from the jugular vein by means of vacuum blood collection tubes with a clotting activator (Vacutest Kima, Arzergrande, Italy), and centrifuged at 1646 g for 3 min to obtain the serum. As a first screening, the sera of all goats and sheep of the selected farms were tested for anti-SRLV antibodies with a commercial kit “Id.Vet” (ID Screen® MVV/CAEV Indirect Screening Test, ID.Vet Innovative Diagnostics, Grables, France). All sera belonging to farms with at least one non-negative animal were tested with a second commercial screening kit “Eradikit Screening” for the detection of anti-SRLV antibodies (EradikitTM SRLV Screening Kit, IN3 Diagnostics, Torino, Italy). All procedures were performed according to manufacturers’ instructions.
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