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Tim 3 5d12

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Tim-3 (5D12) is a monoclonal antibody that specifically binds to the T-cell immunoglobulin and mucin domain-containing protein 3 (Tim-3). Tim-3 is an inhibitory receptor expressed on various immune cells, including T cells, natural killer cells, and dendritic cells. The primary function of this product is to detect and study the expression of Tim-3 on target cell populations.

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Lab products found in correlation

Ef506, by Thermo Fisher Scientific (2 mentions) Pd 1 rmp1 30, by BioLegend (2 mentions) Lag 3 c9b7w, by BioLegend (2 mentions) Tigit gigd7, by BioLegend (2 mentions) Procr ebio1560, by BioLegend (2 mentions) Pdpn 8.1.1, by BioLegend (2 mentions) Ionomycin, by Merck Group (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Lsr 2, by BD (2 mentions)

Spelling variants of this product

TIM-3 (58 citations)

2 protocols using tim 3 5d12

1

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were stained with antibodies against CD4 (RM4–5), CD8 (53–6.7), PD-1 (RMP1–30), Lag-3 (C9B7W), TIGIT (GIGD7), and Tim-3 (5D12), Procr (eBio1560), and Pdpn (8.1.1.) were obtained from BioLegend (San Diego, CA). Fixable viability dye eF506 (eBioscience) was used to exclude dead cells. For intra-cytoplasmic cytokine staining, cells were stimulated with (PMA) (50ng/ml, Sigma-Aldrich, MO), ionomycin (1µg/ml, Sigma-Aldrich, MO). Permeabilized cells were then stained with antibodies against IL-2, TNF-α, IFN-γ or IL-10. All data were collected on a BD LSR II (BD Biosciences) and analyzed with FlowJo software (Tree Star).
Chihara N., Madi A., Kondo T., Zhang H., Acharya N., Singer M., Nyman J., Marjanovic N.D., Kowalczyk M.S., Wang C., Kurtulus S., Law T., Etminan Y., Nevin J., Buckley C.D., Burkett P.R., Buenrostro J.D., Rozenblatt-Rosen O., Anderson A.C., Regev A, & Kuchroo V.K. (2018). Induction and transcriptional regulation of the co-inhibitory gene module in T cells. Nature, 558(7710), 454-459.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were stained with antibodies against CD4 (RM4–5), CD8 (53–6.7), PD-1 (RMP1–30), Lag-3 (C9B7W), TIGIT (GIGD7), and Tim-3 (5D12), Procr (eBio1560), and Pdpn (8.1.1.) were obtained from BioLegend (San Diego, CA). Fixable viability dye eF506 (eBioscience) was used to exclude dead cells. For intra-cytoplasmic cytokine staining, cells were stimulated with (PMA) (50ng/ml, Sigma-Aldrich, MO), ionomycin (1µg/ml, Sigma-Aldrich, MO). Permeabilized cells were then stained with antibodies against IL-2, TNF-α, IFN-γ or IL-10. All data were collected on a BD LSR II (BD Biosciences) and analyzed with FlowJo software (Tree Star).
Chihara N., Madi A., Kondo T., Zhang H., Acharya N., Singer M., Nyman J., Marjanovic N.D., Kowalczyk M.S., Wang C., Kurtulus S., Law T., Etminan Y., Nevin J., Buckley C.D., Burkett P.R., Buenrostro J.D., Rozenblatt-Rosen O., Anderson A.C., Regev A, & Kuchroo V.K. (2018). Induction and transcriptional regulation of the co-inhibitory gene module in T cells. Nature, 558(7710), 454-459.
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