Af s micro nikkor 60 mm f 2.8 g ed lens

The clinical condition of mice (e.g., total wound area, progress of healing process, skin inflammation intensity and mobility) was recorded daily. The animals were weighted periodically. Skin images for photo-documentation were acquired using a Nikon D5100 digital camera (Nikon, Tokyo, Japan) equipped with an AF-S Micro Nikkor 60 mm f/2.8 G ED lens (Nikon, Tokyo, Japan), which was at a fixed distance of 30 cm perpendicular to the subject. The photographs were digitized, and the wound area was measured using Adobe Photoshop C5. Wound closure was defined as a reduction in the wound area and the results were expressed as a percentage (%) of the original wound area.
At the end of the experiment, when total wound closure could be observed for at least one group of treated mice, the mice were sacrificed and skin tissue was excised. Skin sections of specimens from all groups were performed using a paraffin microtome (Shandon Finesse, Thermo Fisher Scientific, Cheshire, UK) and stained with hematoxylin and eosin stain kit (Atom Scientific, Cheshire, UK). Parameters such as inflammation, hyperkeratosis, parakeratosis and skin structure, were estimated.

Wounds were photographed after wound induction and every other day until the end of the experiments using a Nikon D5100 digital camera (Nikon, Tokyo, Japan) equipped with an AF-S Micro Nikkor 60 mm f/2.8 G ED lens (Nikon, Tokyo, Japan), which was fixed at a distance of 20 cm perpendicular to the subject. Pictures were also acquired using an Antera 3D camera (Miravex, Dublin, Ireland). Antera 3D uses an optical method combined with a complex algorithm to capture images in three dimensions. Its software, version 2.11.5, was used to evaluate wound area (mm²) and volume [28 (link)].

A Nikon AF-S Micro-NIKKOR 60 mm f/2.8G ED lens was used to capture project specimens and can approach or achieve a 1:1 magnification ratio or greater for small-bodied specimens. Because reptile and amphibian subjects have wide-ranging body sizes, a 50–100 mm lens is recommended for capturing herpetological specimens with a wet imaging station setup.