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Rabbit anti ampkα 2532

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The Rabbit anti-AMPKα (2532) is a primary antibody that recognizes the alpha subunit of the AMP-activated protein kinase (AMPK) enzyme. AMPK is a key regulator of cellular energy homeostasis and plays a crucial role in various cellular processes.

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Lab products found in correlation

Mouse anti β actin, by Cell Signaling Technology (1 mentions) Rabbit anti gfap, by ABclonal (1 mentions) Nbp1 87842, by Novus Biologicals (1 mentions) Pierce bicinchoninic acid assay, by Thermo Fisher Scientific (1 mentions) Rabbit anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Rabbit anti parp, by Cell Signaling Technology (1 mentions) Mouse anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Rabbit anti phospho ampkα thr172 2535, by Cell Signaling Technology (1 mentions) Rabbit anti 53bp1 4937, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

AMPKα (280 citations) Anti-AMPKα (120 citations) Rabbit anti-AMPKα (22 citations) Anti‐AMPKα (2532) (2 citations)

2 protocols using rabbit anti ampkα 2532

1

Lysis and Immunoblot Analysis of Brain Samples

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Brain samples or cells were lysed on ice for 30 min using a Triton-based lysis buffer containing 50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 1% Triton, as well as protease and phosphatase inhibitors as previously described (16 (link), 25 (link)). After centrifugation at 16,000g, 4 °C for 30 min, the supernatant was collected for protein quantification using the Pierce bicinchoninic acid assay (23227; Thermo). Typically, 5 to 10 μg of total proteins were loaded for each sample on the Invitrogen 4 to 12% Bis–Tris gel, and the following antibodies were used for immunoblot detection: rabbit anti-Synj1 (NBP1-87842; Novus Biologicals; 1:1000), rabbit anti-GFAP (A0237; Abclonal; 1:2000), rabbit anti-pT172 AMPK (2535; Cell Signaling; 1:1000), rabbit anti-AMPKα (2532; Cell Signaling; 1:1000), mouse anti-β-actin (37005; Cell Signaling; 1:3000). All Western blots were performed with two to three technical repeats, and the Western blot bands were analyzed using ImageJ (Rasband, W.S., ImageJ, U. S. National Institutes of Health, https://imagej.nih.gov/ij/).
Pan P.Y., Zhu J., Rizvi A., Zhu X., Tanaka H, & Dreyfus C.F. (2021). Synaptojanin1 deficiency upregulates basal autophagosome formation in astrocytes. The Journal of Biological Chemistry, 297(1), 100873.
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2

AMPK Signaling Pathway Antibodies and Mice

Cited 1 time
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The following antibodies were obtained from Cell Signaling Technology (Beverly, MA): rabbit anti-AMPKα (2532), rabbit anti-phospho-AMPKα (Thr172) (2535), rabbit anti-phospho-histone H3 (Ser10) (9701), rabbit anti-phospho-p53 (Ser18) (9284), mouse anti-p53 (2524), rabbit anti-γ-H2AX (2577), anti-H2AX (2595), anti-phospho-Chk1 (Ser345) (2348), rabbit anti-53BP1 (4937), rabbit anti-cleaved caspase-3 (Asp175) (9664), rabbit anti-PARP (9542), rabbit anti-phospho-Cdk2 (Thr160) (2561), rabbit anti-Cdk2 (2546), rabbit anti-phospho-Cdk1(Thr161) (9114), and mouse anti-cyclin B1 (V152) (4135). The following antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA): goat anti-AMPKα1 (sc-19128), goat anti-AMPKα2 (sc-19129), mouse anti-XRCC4 (sc-365118), mouse anti-p21 (sc-6246), mouse anti-GAPDH (sc-137179) and mouse anti-β-actin (sc-47778). Caspase-3 inhibitor Z-DEVD-FMK (Cat. # FMK004) and Caspase-9 inhibitor Z-LEHD-FMK (Cat. # FMK008) were purchased from R&D Systems, Inc. Other chemicals and organic solvents of the highest available grade were obtained from Sigma-Aldrich. Ampkα1−/− and Ampkα2−/− mice were described elsewhere [28 (link), 29 (link)]. Mice were handled in accordance with study protocols approved by the Institutional Animal Care and Use Committee of the University of Oklahoma Health Sciences Center (Oklahoma City, OK).
Xu H., Zhou Y., Coughlan K.A., Ding Y., Wang S., Wu Y., Song P, & Zou M.H. (2014). AMPKα1 deficiency promotes cellular proliferation and DNA damage via p21 reduction in mouse embryonic fibroblasts. Biochimica et biophysica acta, 1853(1), 65-73.
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