Senegalese sole (20–50 g) were obtained from commercial hatcheries and kept at the aquarium facilities of the University of Santiago de Compostela at 22 °C. Upon arrival, some fish were sacrificed with an anaesthetic overdose (MS-222, tricaine methane sulphonate, Sigma) and used for diagnosis of bacterial pathogens as well as regular viral agents, including infectious pancreatic necrosis virus (IPNV), infectious haematopoietic necrosis virus (IHNV), viral haemorrhagic septicaemia virus (VHSV) and betanodavirus as described by [21 (link)]. All efforts were made to minimize the number of animals used and their suffering.
Tricaine methane sulphonate
Manufactured by Merck Group
Sourced in United States
Tricaine methane sulphonate is a chemical compound commonly used as an anesthetic for fish and amphibians. It functions by reversibly depressing the central nervous system, inducing a state of reduced sensory perception and mobility in the target organism.
Automatically generated - may contain errors
8 protocols using tricaine methane sulphonate
1
Viral Pathogen Screening in Senegalese Sole
2
Fish Growth and Body Indices
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At the end of the eight weeks feeding trial, 24 h after the last feeding, all fish were counted, individually weighed, and their length was measured. Five fish per tank were randomly selected and anesthetised with 80 mg/L tricaine methanesulphonate, (MS‐222, Sigma, St. Louis, MO, USA) then euthanised at − 20 °C for subsequent whole-body proximate analysis. The remaining fish were dissected to obtain tissue samples such as liver, intestine, and fat. The organs were weighed and the body indices [viscerosomatic index (VSI), hepatosomatic index (HSI), intraperitoneal fat index (IPF), and condition factor (CF)] were calculated58 (link)–60 (link) as the percentage of the organ to the whole-body weight of individual fish and used to assess the nutritional status of the fish.
3
Xenopus Oocyte Electrophysiology Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
cRNA was synthesized by in vitro transcription using message machine T7 kits (Ambion, Austin, TX, USA) and injected into stage V and VI oocytes which were surgically isolated from female Xenopus laevis (Nasco, Modesto, CA, USA) anesthetized with 0.17% tricaine methanesulphonate (Sigma, St. Louis, MO, USA). The injected oocytes were maintained in modified Barth`s solution which contained 88 mM NaCl, 1 mM KCl, 0.4 mM CaCl, 0.33 mM Ca(NO3)2, 1 mM MgSO4, 2.4 mM NaHCO3, 10 mM HEPES (pH 7.4), and 50 µg/mL gentamicin sulfate at 17°C. The currents were measured three to six days after injection.
4
Gill Morphology and Transcriptome upon Lead Exposure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals of comparable body dimensions were randomly assigned to the different exposure tanks equipped with aeration devices; each 30 L aquaria (40 × 32 × 20 cm) housed ten fish. The control group was kept in dechlorinated tap water. During the experiment, animals were maintained under the same conditions as before but fed every 48 h. A static exposure system was used following standard procedure guidelines with the renewal of test solutions after 24 h. The use of animals in this study was approved by the Institutional Animal Care and Use Committee at the National University of Entre Rios and the Italian University Institute of Rosario (Rosario, Argentina; protocol N°028/12). For each concentration, including the control, three replicates were performed. After 48, 96, and 192 h of Pb exposure, animals from both control and the exposure groups (n = 10) were deeply euthanized with tricaine methane sulphonate (20 mg/L MS 222 Sigma-Aldrich, St. Louis, MO, USA). Gill samples were excised and promptly cleaned of blood residues for subsequent molecular and morphological analyses. No mortality was recorded in both treated and untreated groups during the experimental period.
5
Anesthetic Evaluation in Seawater
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Benzocaine (Benzoak vet, 200 mg/ml, EuroPharma, Leknes, Norway), metacaine (Tricaine methanesulphonate 1000 mg/g, Sigma Aldrich Co., St. Louis, USA, which is also named Finquel, or MS-222), and isoeugenol (Aqui-S, 540 mg/ml, Scan Aqua, Norway) were used in this study. Liquid Benzoak was stirred into seawater (12.5, 25, 37.5, 50, 100 mg/L), metacaine powder and the corresponding weight of sodium bicarbonate (Na2CO3) were dissolved in seawater at concentrations of 25, 38, 44, 60, 75, 100, and 150 mg/L. Isoeugenol (540 mg/ml) was diluted in ~5 mL heated (30°C) water prior to being added to the seawater to yield 6, 12.5, 18, 25, and 50 mg/L. The selected chemical concentrations were based on results from tests done prior to the experiments.
6
Monogenean Infestation in Arctic Skates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In total, eleven specimens of A. radiata were examined for the presence of monogenean individuals during a field expedition in Spitsbergen, Svalbard, July 2016 organised by the Centre for Polar Ecology (University of South Bohemia, Czech Republic). Host specimens were caught in the Adventfjorden near Hotellneset, Spitsbergen, Svalbard (78°15'18"N, 15°30'58"E) using benthic gill nets at a depth of 30-40 m and immediately transported to the laboratory in seawater containers. Prior to dissection and subsequent examination, skates were euthanised by overdosing with tricaine methane sulphonate (Sigma-Aldrich, Darmstadt, Germany).
7
Bacterial Isolation From Fish Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(Tricaine methane sulphonate, Sigma-Aldrich). Y. ruckeri O1 biotype 2 was re-isolated from head kidney of freshly euthanized fish (2 fish/group) on blood agar plates to confirm that the disease was caused by the challenge strain [14] .
8
Zebrafish Scale Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Danio rerio AB strain was maintained routinely in a ZebTec Bench top (Tecniplast, Italy) under standard condition at 28°C. Six month old male zebrafish of similar weight and length were treated for 2 weeks through directly immersion in a E3 medium solution containing prednisolone 50 μM (Sigma, Italy), alendronate 30 μM (Sigma) or both. To collect scales fish were anaesthetized in 0.01% tricaine methanesulphonate (Sigma) and scales were carefully removed uniformly from the anterior area of either side of the body under a dissecting stereomicroscope (Wild M3Z, Heerbrugg, Switzerland) using Dumont® Stainless Steel Forceps (Sigma). When necessary fish were euthanized using a 0.1% tricaine methanesulphonate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!