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Diua 250

Manufactured by BioAssay Systems
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Sourced in United States

The DIUA-250 is a versatile laboratory equipment designed to perform colorimetric assays. It utilizes a dual-beam optical system to measure the absorbance of samples in a 96-well microplate format. The DIUA-250 is capable of detecting a wide range of analytes and can be used in various applications within the field of biochemistry and molecular biology.

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Lab products found in correlation

Microtainer tube, by BD (4 mentions) Diua 250, by R&D Systems (2 mentions) Mf2100, by BioAssay Systems (2 mentions) Ealt 100, by Crystal Chem (2 mentions) Etga 200, by BioAssay Systems (2 mentions) Eastr 100, by BioAssay Systems (2 mentions) Mob00, by R&D Systems (2 mentions) Mf2100, by R&D Systems (2 mentions) Diur 100, by BioAssay Systems (2 mentions) Ultra sensitive mouse insulin elisa kit 90080, by Crystal Chem (1 mentions) Efru 100, by BioAssay Systems (1 mentions) Mlcn20, by R&D Systems (1 mentions) Dtba 100, by BioAssay Systems (1 mentions)

4 protocols using diua 250

1

Serum and Tissue Biochemical Analyses

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Blood was collected in Microtainer tubes (BD) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13,000 rpm for 2 min at room temperature. Serum parameters were performed biochemically following the manufacturer’s instructions [blood urea nitrogen (BUN): DIUR-100, Bioassay Systems, Hayward, CA, USA; uric acid: DIUA-250, Bioassay Systems; Creatinine: C753291, Pointe Scientific; fructose: EFRU-100, Bioassay Systems]. Urinary neutrophil gelatinase-associated lipoprotein (NGAL; MLCN20, R&D Systems, Minneapolis, MN, USA), and albumin (Albuwell M; Ethos Biosciences) levels were normalized to units of creatinine. Determination of parameters in tissue was performed in freeze-clamped tissues and measured biochemically following the manufacturer’s protocol [uric acid: DIUA-250, Bioassay Systems; Hydroxyproline: DHYP-100, Bioassay Systems; thiobarbituric acid-reactive substances (TBARS): DTBA-100, Bioassay Systems; and uric acid: DIUA-250, Bioassay Systems].
Andres-Hernando A., Orlicky D.J., Cicerchi C., Kuwabara M., Garcia G.E., Nakagawa T., Sanchez-Lozada L.G., Johnson R.J, & Lanaspa M.A. (2023). High Fructose Corn Syrup Accelerates Kidney Disease and Mortality in Obese Mice with Metabolic Syndrome. Biomolecules, 13(5), 780.
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2

Serum and Tissue Biochemical Analysis in Mice

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Blood was collected in microtainer tubes (BD) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13000 rpm for 2 min at room temperature. Serum parameters was performed biochemically following manufacturer’s instruction (uric acid: Bioassay systems, DIUA-250; FGF21: R&D, MF2100, AST: Bioassay Systems, EASTR-100, ALT: Bioassay Systems, EALT-100, Insulin: Crystal Chem, 90080, Leptin: R&D, MOB00, FGF21: R&D, MF2100). Determination of parameters in tissue was performed in freeze-clamped tissues and measured biochemically following manufacturer’s protocol (Triglycerides (Liver): Bioassay Systems, ETGA-200; uric acid: Bioassay Systems DIUA-250).
Andres-Hernando A., Orlicky D.J., Kuwabara M., Ishimoto T., Nakagawa T., Johnson R.J, & Lanaspa M.A. (2020). Deletion of fructokinase in the liver or in the intestine reveals differential effects on sugar-induced metabolic dysfunction. Cell metabolism, 32(1), 117-127.e3.
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3

Comprehensive Mouse Serum Biochemistry

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Blood was collected in Microtainer tubes (BD) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13,000 rpm for 2 minat room temperature. Serum parameters were determined biochemically with commercially available kits following the manufacturer’s instructions Phosphate: K410, Creatine phosphokinase (CPK): K777, Glutamate: K629 and Glucose K606 from Biovision, Milpitas, CA; Creatinine: C7548, Pointe Scientific, Canton, MI; Blood urea nitrogen (BUN): DIUR-100 and Uric Acid: DIUA-250 Bioassay Systems, Hayward, CA; Albumin: Albuwell M (Ethos Biosciences, Logan Township, NJ); Insulin: Ultra-sensitive mouse insulin ELISA kit, 90,080, Crystal Chem, Elk Grove Village, IL.
Andres-Hernando A., Cicerchi C., Garcia G.E., Orlicky D.J., Stenvinkel P., Johnson R.J, & Lanaspa M.A. (2023). Phosphate depletion in insulin-insensitive skeletal muscle drives AMPD activation and sarcopenia in chronic kidney disease. iScience, 26(4), 106355.
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4

Serum and Tissue Biochemical Analyses in Mice

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Blood was collected in microtainer tubes (BD Biosciences) from cardiac puncture of mice under isoflurane, and serum was obtained after centrifugation at 13,148g for 2 minutes at room temperature. Serum parameters was performed biochemically following manufacturer’s instruction (uric acid: BioAssay Systems, DIUA-250; FGF21: R&D, MF2100; AST: BioAssay Systems, EASTR-100; ALT: BioAssay Systems, EALT-100; insulin: Crystal Chem, 90080; leptin: R&D, MOB00; FGF21: R&D, MF2100). Determination of parameters in tissue was performed in freeze-clamped tissues and measured biochemically following manufacturer’s protocol (triglycerides [liver]: BioAssay Systems, ETGA-200; uric acid: BioAssay Systems, DIUA-250).
Andres-Hernando A., Jensen T.J., Kuwabara M., Orlicky D.J., Cicerchi C., Li N., Roncal-Jimenez C.A., Garcia G.E., Ishimoto T., Maclean P.S., Bjornstad P., Sanchez-Lozada L.G., Kanbay M., Nakagawa T., Johnson R.J, & Lanaspa M.A. (2021). Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor. JCI Insight, 6(1), e140848.
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