Anti spop antibody 16750 1 ap

Manufactured by Proteintech

The Anti-SPOP antibody (16750-1-AP) is a primary antibody produced by Proteintech. It recognizes the SPOP (Speckle-type POZ protein) protein. The antibody is intended for research use only.

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Lab products found in correlation

Mg132 bml pi102 0005, by Enzo Life Sciences (2 mentions) Anti p27 sc 527, by Santa Cruz Biotechnology (2 mentions) Anti gst 2625, by Cell Signaling Technology (2 mentions) Anti flag agarose beads a 2220, by Merck Group (2 mentions) Peroxidase conjugated anti rabbit secondary antibody a 4914, by Merck Group (1 mentions) Monoclonal anti ha antibody mms 101p, by BioLegend (1 mentions) Apcin 1 444, by R&D Systems (1 mentions) Anti c myc sc 40, by Santa Cruz Biotechnology (1 mentions) Anti ha antibody sc 805, by Santa Cruz Biotechnology (1 mentions) Anti ha agarose beads a2095, by Merck Group (1 mentions) Anti gfp 8371 2 antibody, by Takara Bio (1 mentions) Anti hdac6 7612, by Cell Signaling Technology (1 mentions) Anti myc tag 2276, by Cell Signaling Technology (1 mentions) Anti tubulin sc 73242, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti spop antibody 16750 1 ap

Antibody Characterization for Protein Analysis

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-PDK1 antibody (8292787) were purchased from BD Biosciences. Anti-SPOP antibody (16750–1-AP) and anti-CK1 antibody (14388–1-AP) were purchased from Proteintech. Anti-pS473-AKT antibody (4060), anti-pT308-AKT antibody (2965), anti-AKT1 antibody (2938), anti-AKT total antibody (4691), anti-Cullin 3 (2759), anti-GST (2625), polyclonal anti-Myc-Tag antibody (2278), anti-GAPDH (5174) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling Technology. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-TRIM24 (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma.

Jiang Q., Zheng N., Bu L., Zhang X., Zhang X., Wu Y., Su Y., Wang L., Zhang X., Ren S., Dai X., Wu D., Xie W., Wei W., Zhu Y, & Guo J. (2021). SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions. Molecular Cancer, 20, 100.

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Delineating the Ubiquitin-Proteasome Pathway

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Anti-Cdc20 (p55 CDC, sc-5296 and sc-13162), anti-Cdh1 (Fzr, sc-56312), anti-p27 (SC-527), anti-HA antibody (SC-805), anti-c-Myc (sc-40), and anti-p27 (SC-527) antibodies were purchased from Santa Cruz. Anti-SPOP antibody (16750-1-AP) was purchased from Proteintech. Mouse monoclonal anti-Myc-Tag (2276), rabbit polyclonal anti-Myc-Tag antibody (2278), anti-Cullin 3 (2759), and anti-GST (2625) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-vinculin antibody (V-4505), anti-tubulin antibody (T5168), peroxidase-conjugated anti-mouse secondary antibody (A-4416), peroxidase-conjugated anti-rabbit secondary antibody (A-4914), anti-HA agarose beads (A-2095) and anti-Flag agarose beads (A-2220) were purchased from Sigma. All antibodies were used in 1:1000 dilutions in 5% non-fat milk for western blot. MLN4924 was a kind gift from Dr. William Kaelin (Dana-Farber cancer institute). MG132 (BML-PI102-0005) was purchased from Enzo life science. Apcin (I-444) was purchased from BostonBiochem.

Wu F., Dai X., Gan W., Wan L., Li M., Mitsiades N., Wei W., Ding Q, & Zhang J. (2016). Prostate cancer-associated mutation in SPOP impairs its ability to target Cdc20 for poly-ubiquitination and degradation. Cancer letters, 385, 207-214.

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Western Blot Antibody Dilutions

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All antibodies were used at 1:1000 dilution in 5% non-fat milk for Western blot. Anti-SPOP antibody (16750-1-AP) was purchased from Proteintech. Anti-Cul3(2759), anti-LATS1(3477) and anti-CK1(12417) antibodies were purchased from Cell Signaling. Anti-Tubulin(T9028), anti-Actin-Peroxidase(A3854), anti-Flag(F1804) and anti-C-Myc(A5598) antibodies were purchased from Sigma. Peroxidase-conjugated anti-mouse secondary antibody (32430) and peroxides-conjugated anti-rabbit secondary antibody(31462) were purchased from Thermo. Anti-HA antibody (sc-805) was purchased from Santa Cruz Biotechnology.

Wang L., Lin M., Chu M., Liu Y., Ma J., He Y, & Wang Z.W. (2020). SPOP promotes ubiquitination and degradation of LATS1 to enhance kidney cancer progression. EBioMedicine, 56, 102795.

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Antibody Detection and Validation Protocol

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Anti-HDAC6 (7612), mouse monoclonal anti-Myc-Tag (2276), rabbit polyclonal anti-Myc-Tag antibody (2278), anti-Cullin 3 (2759), anti-LC3-B (2775), EMT antibody sampler kit (9782), anti-Ac-α-Tubulin (5335) and anti-GST (2625) antibodies were purchased from Cell Signaling. Anti-TRIM24 (TIF1α, SC-271266), anti-Cullin 1 (SC-11384), anti-HA antibody (SC-805), anti-Tubulin (SC-73242) and anti-p27 (SC-527) antibodies were purchased from Santa Cruz. Anti-SPOP antibody (16750-1-AP) was purchased from Proteintech. Anti-GFP (8371-2) antibody was purchased from Clontech. Polyclonal anti-Flag antibody (F7425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-vinculin antibody (V-4505), peroxidase-conjugated anti-mouse secondary antibody (A-4416), peroxidase-conjugated anti-rabbit secondary antibody (A-4914), anti-HA agarose beads (A-2095) and anti-Flag agarose beads (A-2220) were purchased from Sigma. All antibodies were used in 1:1000 dilutions in 5% non-fat milk for western blot.
MLN4924 was a kind gift from Dr. William Kaelin (Dana-Farber cancer institute). Bafilomycin A1 was kindly provided by Dr. Junying Yuan (Harvard Medical School). MG132 (BML-PI102-0005) was purchased from Enzo life science.

Tan Y., Ci Y., Dai X., Wu F., Guo J., Liu D., North B.J., Huo J, & Zhang J. (2017). Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6. Oncotarget, 8(29), 47890-47901.

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