Rat mid osteocalcin eia

Manufactured by Immunodiagnostic Systems

Sourced in United Kingdom, Germany

The Rat-MID Osteocalcin EIA is a laboratory equipment product designed to measure the levels of osteocalcin, a protein found in bone, in rat samples. The assay utilizes an enzyme-linked immunosorbent assay (EIA) technique to quantify the osteocalcin concentration.

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Lab products found in correlation

Ratlaps eia, by Immunodiagnostic Systems (6 mentions) Architect c16000 analyzer, by Abbott (3 mentions) Ratlaps ctx 1 eia, by Immunodiagnostic Systems (2 mentions) Es180s 100, by Calbiotech (2 mentions) Rattrap assay, by Immunodiagnostic Systems (1 mentions) Ac 06f1, by Immunodiagnostic Systems (1 mentions) Ac 12f1, by Immunodiagnostic Systems (1 mentions) Microvue serum pyd eia kit, by Quidel (1 mentions)

12 protocols using rat mid osteocalcin eia

Bone Turnover Markers in Rats

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In the serum obtained on rat sacrifice, concentrations of osteocalcin (a bone formation marker) and C-terminal type I collagen fragments released from bone during bone resorption were determined using enzyme immunoassays (Rat-MID Osteocalcin EIA and RatLaps EIA, respectively, Immunodiagnostic Systems Ltd., Boldon, Tyne and Wear, UK), following the manufacturer’s instructions. Serum levels of fructosamine, total calcium, and total cholesterol were measured spectrophotometrically, using Pointe Scientific, Inc., Canton, MI, USA, kits, according to the manufacturer’s instructions.

Folwarczna J., Janas A., Cegieła U., Pytlik M., Śliwiński L., Matejczyk M., Nowacka A., Rudy K., Krivošíková Z., Štefíková K, & Gajdoš M. (2017). Caffeine at a Moderate Dose Did Not Affect the Skeletal System of Rats with Streptozotocin-Induced Diabetes. Nutrients, 9(11), 1196.

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Bone Metabolism Biomarkers in Rats

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Analyses of alkaline phosphatase (AP), calcium (Ca), magnesium (Mg), and phosphor (P) were conducted at the Department of Clinical Chemistry, University Medical Center, Goettingen using commercial tests (Architect, Abbott, Wiesbaden, Germany) and an automated chemistry analyzer (Architect c16000 Analyzer, Abbott). Osteocalcin (OC) and the cross-linked C-telopeptide of type-I collagen (CTX-I) were assessed using an enzyme-linked immunosorbent assay (EIA), rat-MID™ Osteocalcin EIA, and RatLaps (CTX-I) EIA, respectively (Immunodiagnostic Systems GmbH, Frankfurt am Main, Germany). Follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured by EIA kit for rats (Cloud-Clone Corp., Katy, Texas, USA).

Komrakova M., Büchler G., Böker K.O., Lehmann W., Schilling A.F., Roch P.J., Taudien S., Hoffmann D.B, & Sehmisch S. (2022). A combined treatment with selective androgen and estrogen receptor modulators prevents bone loss in orchiectomized rats. Journal of Endocrinological Investigation, 45(12), 2299-2311.

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Serum Biomarkers of Bone Metabolism

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Rats were fasted overnight before each blood collection by intravenous puncture in the tail at surgery day (D0: start point) and day 14 (D14: intermediate point), and by cardiac puncture under anesthesia at the end of the study (D28: end point). Blood was centrifuged at 13000 g for 2 minutes at room temperature for D0 and D14 samples or at 2000 g for 10 minutes at 4°C for D28 samples and separated in serum and plasma, then stored at −80°C. Serum levels of C‐terminal collagen cross‐linking telopeptide of type I collagen (CTX), procollagen type I N‐terminal propeptide (P1NP), and osteocalcin (Osteocalcin) were quantified by ELISA commercial kits according to manufacturer protocols (Ratlaps CTX‐I EIA kits, Rat/Mouse P1NP EIA kits, and Rat‐MID Osteocalcin EIA, Immunodiagnostic Systems, Paris, France, respectively). Serum calcium and inorganic phosphate levels were measured with colorimetric assays (Kit Biolabo SAS, Maizy, France). In addition, levels of parathyroid hormone (PTH) and fibroblast growth factor 23 (FGF23) in plasma were measured by Rat Intact PTH ELISA Kit (Immunotopics, San Clemente, CA, USA) and Mouse/Rat FGF23 Intact ELISA Kit (Immunotopics), respectively.

Nguyen D.K., Laroche N., Vanden‐Bossche A., Linossier M., Thomas M., Peyroche S., Normand M., Bertache‐Djenadi Y., Thomas T., Marotte H., Vico L., Lafage‐Proust M, & Rousseau M. (2022). Protective Effect on Bone of Nacre Supplementation in Ovariectomized Rats. JBMR Plus, 6(9), e10655.

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Serum Biomarkers of Bone Metabolism

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Serum samples from 6 to 7 FRC, 9 to 10 HRHF‐Untreated, 5 HRHF‐Rest/IgG, and 6 HRHF‐Rest/anti‐CCN2 rats were batch assayed for: (i) osteocalcin (a serum biomarker of osteoblastic activity,^[³⁸, ³⁹, ⁴⁰^] AC‐12F1, Rat‐MID Osteocalcin EIA, Immunodiagnostic Systems, Boldon, Tyne & Wear, UK); (ii) CTX‐1 (C‐telopeptide of type I collagen, a serum biomarker of bone resorption, AC‐06F1, CTX‐I EIA, Immunodiagnostic Systems, USA); (iii) estradiol levels (ES180S‐100, Calbiotech, El Cajon, CA, USA). ELISAs were conducted using manufacturers' protocols, in duplicate. The person carrying out the ELISA analyses was naive to group assignment.

Lambi A.G., Harris M.Y., Amin M., Joiner P.G., Hilliard B.A., Assari S., Popoff S.N, & Barbe M.F. (2023). Blocking CCN2 Reduces Established Bone Loss Induced by Prolonged Intense Loading by Increasing Osteoblast Activity in Rats. JBMR Plus, 7(9), e10783.

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Serum Analysis of Decapitated Rats

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After decapitation, serum samples were collected to detect levels of calcium, magnesium, phosphorus, alkaline phosphatase (Alp) activity, osteocalcin (OC) and RatLaps. The analyses were conducted at the Department of Clinical Chemistry, University of Goettingen using an Architect c16000 analyzer (Abbott, Wiesbaden, Germany) according to the manufacturer´s instructions.
OC was assessed with Rat-MID Osteocalcin EIA (Immunodiagnostic Systems [IDS], Frankfurt am Main, Germany). RatLaps was measured with RatLaps (CTX-I) EIA (IDS).

Saul D., Hohl F.E., Franz M.K., Meyer I., Taudien S., Roch P.J., Sehmisch S, & Komrakova M. (2021). Inhibition of Lipoxygenases Showed No Benefit for the Musculoskeletal System in Estrogen Deficient Rats. Frontiers in Endocrinology, 12, 706504.

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Serum Biomarkers of Bone Metabolism

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Serum concentrations of biochemical markers of bone formation (osteocalcin) and bone resorption (C-terminal telopeptides of type I collagen (CTX-I)) were measured by enzyme immunoassays (Rat-MID Osteocalcin EIA and RatLaps EIA, respectively, produced by Immunodiagnostic Systems Ltd). Serum tartrate-resistant acid phosphatase 5b (TRACP 5b; RatTRAP Assay, Immunodiagnostic Systems Ltd), serum estradiol (Mouse/Rat’s Estradiol ELISA, Calbiotech Inc.), and serum calcitonin gene-related peptide (CGRP; Enzyme-linked Immunosorbent Assay Kit For Calcitonin Gene Related Peptide, USCN Life Science Inc.) levels were also determined. Moreover, serum calcium concentration was measured spectrophotometrically, using a kit produced by Pointe Scientific, Inc.

Janas A, & Folwarczna J. (2016). Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. Naunyn-Schmiedeberg's Archives of Pharmacology, 390(2), 175-185.

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Serum Biomarkers of Bone Metabolism

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Serum samples were batch-assayed for: (1) osteocalcin (a serum biomarker of osteoblastic activity [67 (link),68 (link),69 (link)], AC-12F1, Rat-MID Osteocalcin EIA, Immunodiagnostic Systems, Tyne & Wear, East Boldon, Boldon Colliery, UK); (2) CTX-1 (C-telopeptide of type I collagen, a serum biomarker of bone resorption, AC-06F1, RatLaps EIA, Immunodiagnostic Systems); and (3) TNF-α (tumor necrosis factor alpha, a key pro-inflammatory cytokine, EA100366, Origine, Rockville, MD). ELISAs were conducted using manufacturers’ protocols, in duplicate, by one individual who was naïve to group assignments.

Barbe M.F., Amin M., Harris M.Y., Panibatla S.T., Assari S., Popoff S.N, & Bove G.M. (2022). Manual Therapy Facilitates Homeostatic Adaptation to Bone Microstructural Declines Induced by a Rat Model of Repetitive Forceful Task. International Journal of Molecular Sciences, 23(12), 6586.

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Bone Turnover Markers in Rat Serum

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Measuring the concentration of osteocalcin and cross-linked N-terminal telopeptide of collagen alpha I chain was performed on blood serum collected from rats' tails before beginning the exposure to electromagnetic fields and after 1, 3, and 4 weeks since the beginning of the exposure to electromagnetic field. Measuring the concentration of osteocalcin, cross-linked N-terminal telopeptide of collagen alpha I chain, and pyridinoline was performed additionally on blood serum collected from left ventricle directly before killing the animals. The concentration of the aforementioned markers of bone turnover in serum was measured using colorimetric immunoenzymatic method (ELISA), using the following tests: Rat-MID Osteocalcin EIA (Immunodiagnostic Systems), Osteomark NTx Serum ELISA (Osteomark), and MicroVue Serum PYD EIA Kit (Quidel).

Sieroń-Stołtny K., Teister Ł., Cieślar G., Sieroń D., Śliwinski Z., Kucharzewski M, & Sieroń A. (2015). The Influence of Electromagnetic Radiation Generated by a Mobile Phone on the Skeletal System of Rats. BioMed Research International, 2015, 896019.

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Bone Metabolism Biomarker Analysis

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Serum concentrations of osteocalcin (a marker of bone formation) and C-terminal telopeptide fragments of type I collagen (CTX-I; a marker of bone resorption) were measured by enzyme immunoassays (Rat-MID Osteocalcin EIA and RatLaps EIA, respectively, Immunodiagnostic Systems, Boldon, Tyne and Wear, UK). Serum total calcium and inorganic phosphorus concentrations were measured spectrophotometrically, using kits produced by Pointe Scientific, Canton, MI, USA. The manufacturers’ instructions were followed.

Folwarczna J., Konarek N., Freier K., Karbowniczek D., Londzin P, & Janas A. (2019). Effects of loratadine, a histamine H1 receptor antagonist, on the skeletal system of young male rats. Drug Design, Development and Therapy, 13, 3357-3367.

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Serum Biomarkers of Bone Metabolism

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Alkaline phosphatase (ALP), magnesium, calcium, and phosphor were measured in serum at the Department of Clinical Chemistry, University Medical Center, Goettingen, Germany. Analyses were performed using an automated chemistry analyzer (Architect c16000 analyzer, Abbott, Wiesbaden, Germany) and commercially available kits (Architect, Abbott) according to the manufacturer’s instructions. Osteocalcin (OC) and Cross Linked C-telopeptide of Type I Collagen (CTX-I) were assessed using commercial analysis kits, rat-MID™ Osteocalcin EIA and RatLaps (CTX-I) EIA (Immunodiagnostic Systems GmbH, Frankfurt am Main, Germany).

Komrakova M., Nagel J., Hoffmann D.B., Lehmann W., Schilling A.F, & Sehmisch S. (2020). Effect of Selective Androgen Receptor Modulator Enobosarm on Bone Healing in a Rat Model for Aged Male Osteoporosis. Calcified Tissue International, 107(6), 593-602.

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