Sciex x500r mass spectrometer

A specimen of Phorbas sp. (voucher number 07G-26) was collected by hand from Gageo Island, South Korea, in 2007. Optical rotations were measured on a JASCO P-1010 polarimeter (Jasco, Easton, MD, USA). IR spectra were recorded using a JASCO FT/IR 4100 spectrometer (Jasco, Easton, MD, USA), and ultraviolet (UV) spectra using a Varian Cary 50 UV-Visible spectrophotometer (Agilent, Santa Clara, CA, USA). High-resolution (HR)-electrospray ionization (ESI) mass spectra were obtained using a SCIEX X500R mass spectrometer (Sciex, Framingham, MA, USA). The NMR spectra were recorded on a Varian VNMRS 500 NMR spectrometer (Varian, Palo Alto, CA, USA) operating at 500 (¹H) or 125 MHz (¹³C), respectively, with chemical shifts given in ppm using a methanol-d4 solution concerning residual solvent peaks at 3.30 and 49.0 ppm. Semi-preparative liquid chromatography was performed using an Agilent 1200 pump (Agilent, Santa Clara, CA, USA) and an RI detector. Vacuum column chromatography was performed using RP-18 silica gel 60 (Merck, Darmstadt, Germany).

The optical rotation values were measured using a JASCO P-1010 polarimeter (Jasco, Easton, MD, USA). IR spectra were recorded on a JASCO FT/IR 4100 spectrometer (Jasco, Easton, MD, USA), and ultraviolet (UV) spectra were recorded using a Varian Cary 50 UV-visible spectrophotometer (Agilent, Santa Clara, CA, USA). High-resolution (HR)-electrospray ionization (ESI) mass spectra were measured using a SCIEX X500R mass spectrometer (Sciex, Framingham, MA, USA). Nuclear magnetic resonance (NMR) spectra were recorded on a Varian VNMRS 500 NMR spectrometer (Varian, Palo Alto, CA, USA), operating at 500 MHz (¹H) and 125 MHz (¹³C), with chemical shifts of the proton and carbon spectra measured in methanol-d₄ solution, were reported in reference to residual solvent peaks at 3.30 ppm and 49.0 ppm, respectively. Semi-preparative liquid chromatography (SemiPrep-LC) was performed using a Waters 515 pump (Agilent, Santa Clara, CA, USA) equipped with an RI detector. Column chromatography was performed using an RP-18 silica gel 60 (Merck, Darmstadt, Germany) and Sephadex LH-20 (Pharmacia, Uppsala, Sweden).