HepG2 cells (JCRB1054) were purchased from the Health Science Research Resources Bank (Japan Health Sciences Foundation, Osaka, Japan) and maintained at 37 °C under an atmosphere of 95% air and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were immersed in a culture medium containing the indicated concentrations of Zebularine, 5-Aza-2′-deoxycytidine (5-aza-dC), 2-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)-3-(1H-indol-3-yl) propionic acid (RG108) or PKR inhibitor. Zebularine (Wako Pure Chemical Industries, Osaka, Japan) was dissolved in distilled water as a stock solution. 5-Aza-2′-deoxycytidine, RG108 (Wako Pure Chemical Industries), PKR inhibitor (Merck Millipore, Tokyo, Japan), acetaminophen (Sigma-Aldrich Japan, Tokyo, Japan) and aflatoxin B1 (Wako Pure Chemical Industries) were each dissolved in DMSO as a stock solution.
Rg108
Manufactured by Fujifilm
Sourced in Japan
The RG108 is a digital film and plate processor designed for use in medical and industrial imaging applications. It provides high-quality processing of a variety of film and plate formats. The RG108 is capable of processing a wide range of materials and is built to deliver reliable and consistent results.
Automatically generated - may contain errors
Lab products found in correlation
Acetaminophen, by Merck Group (1 mentions)
Pkr inhibitor, by Merck Group (1 mentions)
Zebularine, by Fujifilm (1 mentions)
5 aza 2 deoxycytidine, by Fujifilm (1 mentions)
Aflatoxin b1, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Retinoic acid, by Merck Group (1 mentions)
4 aminopyridine, by Merck Group (1 mentions)
Bicuculline, by Fujifilm (1 mentions)
Cytosine β d arabinofuranoside hydrochloride, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions)
Tetrodotoxin, by Abcam (1 mentions)
2 protocols using rg108
1
HepG2 cells epigenetic modulation
2
Neuronal Isolation and Manipulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Hippocampal neurons were isolated from embryonic day (E) 17 mice (male and female), according to a previously described protocol [40 (link)]. Cytosine
β-d-arabinofuranoside hydrochloride (Sigma-Aldrich, St. Louis, MO, USA; catalog no. 855855) was added 1 d after plating, to eliminate proliferating, undifferentiated, and glial cells. To
inhibit DNMT activity, the neurons were treated with 100 μM RG108 (Wako, Tokyo, Japan; catalog no. 041-30101) overnight. To suppress spontaneous neuronal activation, the neurons were treated
with 1 μM tetrodotoxin (Abcam, Cambridge, UK; catalog no. ab120055), from 1 to 10 DIV. To induce neuronal activation, 10 DIV neurons were treated with 50 µM bicuculline (Wako; catalog no.
026-16131) and 200 μM 4-aminopyridine (Sigma-Aldrich; catalog no. 275875) for 3 or 6 h. We confirmed the cell viability after neuronal activation by observing the rare presence of active
caspase 3-positive cells (Supplementary Fig. 10 ). Lentiviruses were produced as described previously [41 (link)]. The virus was introduced into the neurons at 6 DIV.
Mouse neuroblastoma Neuro2a cells were cultured in DMEM (Nacalai Tesque, Kyoto, Japan; catalog no. 08458-45) supplemented with 10% FBS (Sigma-Aldrich; catalog no. F7524) and 20 µM retinoic
acid (Sigma-Aldrich; catalog no. R2625).
β-d-arabinofuranoside hydrochloride (Sigma-Aldrich, St. Louis, MO, USA; catalog no. 855855) was added 1 d after plating, to eliminate proliferating, undifferentiated, and glial cells. To
inhibit DNMT activity, the neurons were treated with 100 μM RG108 (Wako, Tokyo, Japan; catalog no. 041-30101) overnight. To suppress spontaneous neuronal activation, the neurons were treated
with 1 μM tetrodotoxin (Abcam, Cambridge, UK; catalog no. ab120055), from 1 to 10 DIV. To induce neuronal activation, 10 DIV neurons were treated with 50 µM bicuculline (Wako; catalog no.
026-16131) and 200 μM 4-aminopyridine (Sigma-Aldrich; catalog no. 275875) for 3 or 6 h. We confirmed the cell viability after neuronal activation by observing the rare presence of active
caspase 3-positive cells (
Mouse neuroblastoma Neuro2a cells were cultured in DMEM (Nacalai Tesque, Kyoto, Japan; catalog no. 08458-45) supplemented with 10% FBS (Sigma-Aldrich; catalog no. F7524) and 20 µM retinoic
acid (Sigma-Aldrich; catalog no. R2625).
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