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4k ccd camera

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The 4K CCD camera is a high-resolution imaging device that captures detailed visual data. It features a charge-coupled device (CCD) sensor capable of recording images at a resolution of 4096 x 2160 pixels, commonly referred to as 4K. The camera is designed to provide accurate and reliable image capture for various applications.

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Lab products found in correlation

Formvar carbon coated cu grids, by Ted Pella (2 mentions) Sphera transmission electron microscope, by Ametek (2 mentions) Glow discharged carbon coated copper grid, by Electron Microscopy Sciences (2 mentions) Tecnai t12 electron microscope, by Philips (2 mentions) 1k ccd camera, by Ametek (2 mentions) Epu software, by Thermo Fisher Scientific (2 mentions) Solarus model 950 cleaner, by Ametek (2 mentions) Tecnai t12 electron microscope, by Thermo Fisher Scientific (2 mentions) Vitrobot, by Thermo Fisher Scientific (1 mentions) Tecnai f20 electron microscope, by Thermo Fisher Scientific (1 mentions) Ptp1b, by Abcam (1 mentions) Morgagni 268 tem, by Ametek (1 mentions) Amino acids, by GL Biochem (1 mentions) Tcs sp2 spectral confocal microscope, by Leica (1 mentions) Jem 1200ex tem, by Ametek (1 mentions) H 7650, by Hitachi (1 mentions) Paraformaldehyde (pfa), by Sinopharm (1 mentions) Glutaraldehyde, by Sinopharm (1 mentions) Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions) Filter paper, by Cytiva (1 mentions)

15 protocols using 4k ccd camera

1

Characterization of BMC Cages

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A 4-μL droplet of BMC cages (either self-assembled or from dissolved crystals) was deposited onto formvar/carbon-coated Cu grids (Ted Pella, Inc.) (pretreated by negative-mode glow discharge up to 15 min prior) and allowed to bind for 5 min. The grids were then washed with 50 μL of MilliQ water, blotted using Whatman filter paper and stained using 2% uranyl acetate solution in water and blotted again. Grids were imaged using a FEI Sphera transmission electron microscope operating at 200 keV, equipped with a LaB6 filament and a Gatan 4K CCD camera. Micrographs were collected using objective-lens underfocus settings ranging from 250 nm to 2 μm and analyzed using Fiji (http://fiji.sc/Fiji).
Golub E., Subramanian R.H., Esselborn J., Alberstein R.G., Bailey J.B., Chiong J.A., Yan X., Booth T., Baker T.S, & Tezcan F.A. (2020). Constructing Protein Polyhedra via Orthogonal Chemical Interactions. Nature, 578(7793), 172-176.
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2

Negative Staining of RodA:PBP2 Complex

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For negative staining, 2.5 μl protein solution consisting of RodA:PBP2 complex at a concentration of 0.005 mg ml−1 in 0.01% DDM (w/v) was added to a glow-discharged carbon-coated copper grid (Electron Microscopy Science) and allowed to adsorb for 30 s. Grids were then washed twice with deionized water and stained twice with freshly prepared 1.5% (w/v) uranyl formate. Filter paper was applied to the grid to absorb residual liquid between each step. Samples were then allowed to dry for approximately 2 min. Images used to generate the 2D classes shown in Figure 3 were collected at room temperature using a Philips Tecnai T12 electron microscope equipped with an LaB6 filament and operated at 120 kV. Images were collected at a magnification of 67,000-fold, corresponding to a pixel size of 1.68 Å, and a defocus value of −1.5 μm on a Gatan 4K CCD camera using a low-dose collection procedure. Approximately 1000 particles were manually picked in Relion36 (link), then an auto-picking routine picked the remaining particles for a total of 32,152. Two-dimensional class averages were calculated with Relion. Similar class averages were observed when calculated with EMAN237 (link).
Sjodt M., Rohs P.D., Gilman M.S., Erlandson S.C., Zheng S., Green A.G., Brock K., Taguchi A., Kahne D., Walker S., Marks D.S., Rudner D.Z., Bernhardt T.G, & Kruse A.C. (2020). Structural coordination of polymerization and crosslinking by a SEDS-bPBP peptidoglycan synthase complex. Nature microbiology, 5(6), 813-820.
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3

Cryo-Electron Microscopy of Outer Membrane Vesicles

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Holey carbon film-covered 300 mesh copper grids (HC300-Cu, PELCO) were glow-discharged in an (Ar, O2)-atmosphere for 10 s on the carbon side. Samples (1–10 mg ml−1) of 4 μl volumes were pipetted onto grids. Grids were blotted in 100% humidity at 4 °C for 3 s and plunge-frozen into liquid ethane cooled by liquid nitrogen using a Vitrobot (FEI, Hillsboro, OR). Liquid nitrogen was used to store grids. Grids were then transferred to the electron microscope using a cryostage. Images of OMVs within the holes in the carbon film were obtained by using a Tecnai F20 electron microscope (FEI) at 200 keV with a 70 μm objective aperture. For each exposure, the low dose condition was ~20e− Å−2. Images were taken at 5k or 50k magnification and 2–3 μm defocus and recorded on a 4k × 4k CCD camera (Gatan, USA).
Jan H.M., Chen Y.C., Yang T.C., Ong L.L., Chang C.C., Muthusamy S., Abera A.B., Wu M.S., Gervay-Hague J., Mong K.K, & Lin C.H. (2020). Cholesteryl α-D-glucoside 6-acyltransferase enhances the adhesion of Helicobacter pylori to gastric epithelium. Communications Biology, 3, 120.
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4

HPLC Purification and Characterization of Biomolecules

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ALP was purchased from Biomatik, NBD-Cl from TCI and amino acids from GL
Biochem, PP1 from Sigma-Aldrich, PTP1B from Abcam. All the solvents and chemical reagents
were used directly as received from the commercial sources without further purification.
All the products were purified with Water Delta600 HPLC system, equipped with an XTerra
C18 RP column and an in-line diode array UV detector. Rheological data were obtained on TA
ARES G2 rheometer with 25 mm cone plate, confocal microscopy images on Leica TCS SP2
spectral confocal microscope. Electron microscopy imaging was performed on a FEI Morgagni
268 TEM with a 1k CCD camera (GATAN, Inc., Pleasanton, CA, USA) or a 300 keV Tecnai F30
intermediate voltage TEM (FEI, Inc., Hillsboro, OR, USA) with a 4k CCD camera (GATAN).
Zhou J., Du X., Berciu C., He H., Shi J., Nicastro D, & Xu B. (2016). Enzyme-Instructed Self-Assembly for Spatiotemporal Profiling of the Activities of Alkaline Phosphatases on Live Cells. Chem, 1(2), 246-263.
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5

Transmission Electron Microscopy Imaging

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Multiple ultrathin sections (of 70–80 nm) were cut on a Reichart Ultracut E, collected on Formvar coated copper slot grids, post-stained with uranyl acetate (saturated solution) and Reynold’s lead citrate, and initially imaged at a JEOL JEM-1200EX TEM with a 1k CCD camera (GATAN) in order to get overview maps of the sections and localize the cells of interest. Then, for high-resolution images of these cells, we imaged them at a 200 kV Tecnai F20 intermediate voltage TEM (FEI, Inc., Hillsboro, OR, USA) with a 4k CCD camera (GATAN), at 19,000× magnification (1.12 nm pixel size). For large overviews of the cells at medium magnification, we acquired montages of overlapping images in an automated fashion using the microscope control software SerialEM.60 (link)
Feng Z., Wang H., Wang F., Oh Y., Berciu C., Cui Q., Egelman E.H, & Xu B. (2020). Artificial Intracellular Filaments. Cell reports. Physical science, 1(7), 100085.
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6

Structural Analysis of NA-Fab Complexes

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Recombinant NA and Fabs were diluted with buffer (5mM HEPES, 150 mM NaCl pH 7.3) to approximately 0.02 mg/mL and 0.04 mg/mL respectively. To prepare Fab bound samples, equal volumes of NA and Fabs 1F2 or 4F11 were mixed and incubated for 5–10 minutes. The samples were adsorbed to plasma cleaned (Solarus Model 950 cleaner, Gatan Inc., Pleasanton, CA) EM grids coated with continuous carbon film that were subsequently washed with buffer and stained with 0.75% uranyl formate. Images were collected using EPU software (FEI Company, Hillsboro, OR) on a Tecnai T12 electron microscope (FEI Company) fitted with a 4K CCD camera (Gatan Inc.) at an effective pixel size of 0.18 nm in the specimen plane. The software package RELION 1.4 (50 (link)) was used to obtain 3D reconstructions. The maps for unbound NA, and for the complexes with 1F2, and 4F11 were constructed using 47,592, 4,326, and 13,665 particles, respectively and visualized using UCSF Chimera software (51 (link)).
Wohlbold T.J., Podolsky K.A., Chromikova V., Kirkpatrick E., Falconieri V., Meade P., Amanat F., Tan J., tenOever B.R., Tan G.S., Subramaniam S., Palese P, & Krammer F. (2017). Broadly protective murine monoclonal antibodies against influenza B virus target highly conserved neuraminidase epitopes. Nature microbiology, 2(10), 1415-1424.
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7

Ultrastructural Analysis of bMECs Infected with K. pneumoniae

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Cultured bMECs in six-well plates were challenged with HB-AF5 or HLJ-D2 K. pneumoniae (MOI = 5), with noninfected bMECs similarly incubated as a control. At 6 hpi, bMECs were washed with PBS (pH 7.2), fixed with 2% glutaraldehyde and 1% paraformaldehyde (pH 7.2; Sinopharm Chemical Reagent Co., Shanghai, China) for 45 min at room temperature and processed for TEM [15 (link)]. After washing with PBS, the fixed cells were harvested with a rubber scraper (Thermo Fisher Scientific). Then, cells were dehydrated using graded ethanol and acetone (three changes, 10 min each) and sequentially embedded in epoxy resin acetone mixtures (2:1) for 2 h and then in pure resin overnight at 37 °C. After resin had polymerized, ultra-thin sections were cut (Leica EM, Wetzlar, Germany), stained with 1% uranyl acetate followed by lead citrate, and viewed with a transmission electron microscope (Hitachi H-7650) at 80 kV. Imaging was performed using a 4 k CCD camera (Gatan Inc., Pleasanton, CA, USA) and iTEM software.
Cheng J., Zhang J., Yang J., Yi B., Liu G., Zhou M., Kastelic J.P., Han B, & Gao J. (2021). Klebsiella pneumoniae infection causes mitochondrial damage and dysfunction in bovine mammary epithelial cells. Veterinary Research, 52, 17.
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8

Structural Analysis of NA-Fab Complexes

Cited 1 time
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Recombinant NA and Fabs were diluted with buffer (5mM HEPES, 150 mM NaCl pH 7.3) to approximately 0.02 mg/mL and 0.04 mg/mL respectively. To prepare Fab bound samples, equal volumes of NA and Fabs 1F2 or 4F11 were mixed and incubated for 5–10 minutes. The samples were adsorbed to plasma cleaned (Solarus Model 950 cleaner, Gatan Inc., Pleasanton, CA) EM grids coated with continuous carbon film that were subsequently washed with buffer and stained with 0.75% uranyl formate. Images were collected using EPU software (FEI Company, Hillsboro, OR) on a Tecnai T12 electron microscope (FEI Company) fitted with a 4K CCD camera (Gatan Inc.) at an effective pixel size of 0.18 nm in the specimen plane. The software package RELION 1.4 (50 (link)) was used to obtain 3D reconstructions. The maps for unbound NA, and for the complexes with 1F2, and 4F11 were constructed using 47,592, 4,326, and 13,665 particles, respectively and visualized using UCSF Chimera software (51 (link)).
Wohlbold T.J., Podolsky K.A., Chromikova V., Kirkpatrick E., Falconieri V., Meade P., Amanat F., Tan J., tenOever B.R., Tan G.S., Subramaniam S., Palese P, & Krammer F. (2017). Broadly protective murine monoclonal antibodies against influenza B virus target highly conserved neuraminidase epitopes. Nature microbiology, 2(10), 1415-1424.
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9

Characterization of BMC Cages

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A 4-μL droplet of BMC cages (either self-assembled or from dissolved crystals) was deposited onto formvar/carbon-coated Cu grids (Ted Pella, Inc.) (pretreated by negative-mode glow discharge up to 15 min prior) and allowed to bind for 5 min. The grids were then washed with 50 μL of MilliQ water, blotted using Whatman filter paper and stained using 2% uranyl acetate solution in water and blotted again. Grids were imaged using a FEI Sphera transmission electron microscope operating at 200 keV, equipped with a LaB6 filament and a Gatan 4K CCD camera. Micrographs were collected using objective-lens underfocus settings ranging from 250 nm to 2 μm and analyzed using Fiji (http://fiji.sc/Fiji).
Golub E., Subramanian R.H., Esselborn J., Alberstein R.G., Bailey J.B., Chiong J.A., Yan X., Booth T., Baker T.S, & Tezcan F.A. (2020). Constructing Protein Polyhedra via Orthogonal Chemical Interactions. Nature, 578(7793), 172-176.
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10

TEM Imaging of Self-Assembled Micelles

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TEM imaging of the self-assembled and complexed micelles was performed
on a Philips CM120 transmission electron microscope using a LaB6 filament and operated at an accelerating voltage of 120 kV.
Images were recorded using a Gatan 4 k CCD camera. TEM grids (copper,
400 mesh with a carbon support film) were glow-discharged prior to
sample preparation (15 s at 40 mA and 300 V). Specimens were prepared
by deposition of 5 μL of the micellar solution (c = 0.3 g L–1 in ethanol for PS-b-PAA micelles and c = 0.6 g L–1 in buffer for C3Ms) onto the grid and adsorption for 1 min before
blotting. Before the specimen was fully dried, 5 μL of 2 wt
% uranyl acetate staining solution was deposited onto the grid; this
was immediately blotted, and a new 5 μL drop of staining solution
was deposited and left to adsorb for 1 min before blotting. TEM images
were analyzed using ImageJ software, employing brightness and contrast
correction tools to enhance the general quality of the snapshots and
calculate the average particle size (including its mean standard deviation).
Maan A.M., Graafsma C.N., Hofman A.H., Pelras T., de Vos W.M, & Kamperman M. (2023). Scalable Fabrication of Reversible Antifouling Block Copolymer Coatings via Adsorption Strategies. ACS Applied Materials & Interfaces, 15(15), 19682-19694.
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