Total antioxidant capacity assay kit with the abts method

An ABTS+ radical scavenging assay was conducted using a total antioxidant capacity assay kit with the ABTS method (Beyotime Institute of Biotechnology, Shanghai, China). An ABTS+ radical solution was prepared by oxidizing the reagents with ABTS+ and oxidation solution with a volume ratio of 1:1. The mixture was allowed to stand in the dark at room temperature overnight. Different SSE concentrations (0.0625, 0.125, 0.25, 0.5, and 1 mg/mL) were mixed with 0.2 mL of ABTS+ radical solution for 10 min. Distilled water was a substitute for the SSE sample as the background (absorption control), and AA was used as a positive control. The remaining ABTS+ radical was analyzed on a plate reader (Molecular Devices) at 620 nm.

Chitosan (Cs), sodium alginate (Alg), and kappa carrageenan (κ-Car) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Dextran sodium sulfate (DSS) was supplied from MP Biomedicals (Irvine, CA, United States). MSZ, β-CD, and HP-β-CD were purchased from Solarbio (Beijing, China). Male C57BL/6 mice (8 week old, 18–22 g) were provided by Pengyue Laboratory Animal Technology Co., Ltd. (Jinan, China). Myeloperoxidase (MPO) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The Cell Counting Kit-8 (CCK-8) was obtained from Dongren Chemical Technology Co., Ltd. (Shanghai, China). The hematoxylin and eosin (H&E) staining kit was purchased from Solarbio (Beijing, China). The total antioxidant capacity assay kit with the ABTS method was obtained from Beyotime Biotechnology. Rhodamine B isothiocyanate (RBITC) was purchased from Macklin (Shanghai, China).

Based on the method of Bradford [15 (link)], the protein concentrations of the samples in the A, B, C and D groups were measured using a Quick Start^TM Bradford Protein Assay (Bio-Rad, Hercules, CA, USA). Furthermore, a Total Antioxidant Capacity Assay Kit with the ABTS method (Beyotime Biotechnology, Shanghai, China) was employed to determine the total antioxidant capacity of the samples in the different groups according to the manufacturer’s instructions.